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Articles by N.A.H. Aly
Total Records ( 1 ) for N.A.H. Aly
  E.A.M. Soliman , N.A.H. Aly and M.E. Moharam
  Gram-positive Staphylococcus aureus, Bacillus subtilis and Gram-negative Pseudomonas fluorescens were cured from their own plasmids using an elevated temperature or acridine orange. Elevated temperature was more efficient than acridine orange. Plasmid profiles were varied in size, number and their existence. However, B3 cured strain resulted of B. subtilis by elevated temperature changed their sensitivity profile and became resistant to kanamycin and tetracycline. Similar change was found in P1, P2 and P3 cured strains resulted of P. fluorescens by the two curing agents and became resistant to ampicillin and chloramephincol. The relationship between the presence of plasmid and their antimicrobial resistance was detected in cured strains by acridine orange. Whereas, S1 and S2 cured strains resulted of S. aureus that harboring three plasmids of its original strain were altered their resistance and become sensitive to kanamycin and rifampicin. B1 and B2 cured strains that harboring two plasmids of its original strain showed the similar manner to neomycin. On the other hand, elevated temperature severely affected the enzyme productivity in cured strains and thereby loss their activities while hyperactivities were observed by acridine orange. For instance, S1 and S2 cured strains recorded 14.9 and 10.7 U mL-1 of alkaline protease while the original strain lacked the ability to produce the enzyme. In contrast, B1 cured strain revealed hyperactivity that reached around 14-fold increase in lipase activity with 102.3 U mL-1 than the original strain whereas S1 cured strain reached around 4.2-fold increase with 192 U mL-1 and P1 cured strain reached around 2-fold increase with 69.3 U mL-1. S3 and B3 cured strains were reduced one and two protein bands comparing with the other produced by acridine orange. However, P3 cured strain revealed high total protein bands similar to the original strain. Moreover, the newly induced protein bands were higher in the cured strains by temperature than acridine orange.
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