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Articles by N. Sharma
Total Records ( 3 ) for N. Sharma
  A.A. Khan , P. Peiris , N. Sharma , G. Maddini , V. Raghvan and C. Courteau
  Amylases are extracellular enzymes of great industrial interest. Present study was planned with the objectives- comparison and screening of best α-amylase producer bacteria, effect of PEG 4000 in different concentrations on α-amylase production and, recovery of α-amylase from crude extract, concentrate and ultra-filtrate. It was found that Bacillus amyloliquefaceins had 39.5 mm and Bacillus subtilis 29.5 mm diameter of zone of clearance on starch agar medium, while the isolate from soil showed much lesser (5.5 mm) zone of clearance. Therefore, Bacillus amyloliquefaceins strain was best α-amylase producer than others. Medium with 7 and 2% PEG concentration showed 30 times higher alpha amylase activity than medium without PEG. Furthermore lower concentration of PEG 2% showed promising results as compared to PEG 7%. The highest concentration of α-amylase (2690 U mL-1 of cf) was found in sample concentrate, which is nearly ten times higher than the original sample (260 U mL-1). Theoretically ultra filtrate should not have any amylase activity but in our result it was 89 U mL-1. Present study suggests that B. amyloliquefaceins is the best bacterial strain to produce maximum quantity of α-amylase in the medium containing PEG 2%.
  N. Sharma , K.P. Sharma , R.K. Gaur and V.K. Gupta
  Plants represent the major component of biota and have the capability to synthesize their food through the process of photosynthesis. Physiological and environmental changes affect their health and make them vulnerable to variety of diseases thus directly or indirectly affect other components of ecosystem. A large number of environmental issues are linked with the eradication of plant diseases with chemical compounds. Most of these diseases are caused by fungal and insect pathogens. Chitin is the main structural component of these organisms and thus the enzyme responsible to hydrolyze chitin content are receiving attention in regard to their development as biopesticides or chemical defense proteins in transgenic plants and in microbial biocontrol agents. Therefore, understanding the overview of chitinase will provide a basis for improving the pathogenic activity of potential biocontrol strains, for developing novel biological control strategies and for exploring their roles in the plant defense. The present review describes the properties of chitinase with respect to plant health improvement.
  Yogesh Sharma , N. Sharma , G.V. Subba Rao and B.V.R. Chowdari
 

Nano-CdSnO3 is prepared by thermal decomposition of the precursor, CdSn(OH)6 at 600 °C for 6 h in air. The material is characterized physically by X-ray diffraction (XRD), high-resolution transmission electron microscopy (HR-TEM) and selected-area electron diffraction (SAED) techniques. Nano-CdSnO3 exhibits a reversible and stable capacity of 475(±5) mAh g-1 (5 mol of cycleable Li per mole of CdSnO3) for at least 40 cycles between 0.005 and 1.0 V at a current rate of 0.13 C. Extensive capacity fading is found when cycling in the range 0.005–1.3 V. Cyclic voltammetry studies complement galvanostatic cycling data and reveal average discharge and charge potentials of 0.2 and 0.4 V, respectively. The proposed reaction mechanism is supported by ex situ XRD, TEM and SAED studies. The electrochemical impedance spectra taken during 1st and 10th cycle are fitted with an equivalent circuit to evaluate impedance parameters and the apparent chemical diffusion coefficient (DLi+) of Li. The bulk impedance, Rb, dominates at low voltages (≤0.25 V), whereas the combined surface film and charge-transfer impedance (R(sf+ct)) and the Warburg impedance dominate at higher voltages, ≥0.25 V. The DLi+ is in the range of (0.5–0.9) × 10-13 cm2 s-1 at V = 0.5–1.0 V during the 10th cycle.

 
 
 
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