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Articles by N. Parveen
Total Records ( 2 ) for N. Parveen
  Syyeda Anees , N. Parveen , Siraj Mohammed and Mohammed Ishaq
  Oxidative stress and antioxidant status was evaluated in relation to glycemic control in Type 1 and 2 Diabetes Mellitus patients (DM). A total of 69 DM patients (34 T1 and 35 T2DM cases) were enrolled in the study along with 15 healthy subjects. The patients were grouped into those with good glycemic control and others with poor glycemic control. Serum fructosamine levels were also determined as a supportive parameter for confirming glycemic status. Serum Malondialdehyde (MDA) level was used as a marker of oxidative stress and serum catalase activity was quantitated for an assessment of anti-oxidant status of the patients. Mean MDA levels in poor glycemic control group of T1 and T2DM were significantly higher (p<0.01) not only than the means of the healthy controls but also than the means of diabeteic groups with good glycemic control (p<0.01). Mean serum catalase activity was significantly reduced in DM patients of all the groups compared to that of healthy controls (p<0.05) indicating considerable reduction in the anti-oxidant status of the patients.
  K. A Cornell , S Primus , J. A Martinez and N. Parveen
  Background

Lyme disease is the most prevalent tick-borne disease in the USA with the highest number of cases (27 444 patients) reported by CDC in the year 2007, representing an unprecedented 37% increase from the previous year. The haematogenous spread of Borrelia burgdorferi to various tissues results in multisystemic disease affecting the heart, joints, skin, musculoskeletal and nervous system of the patients.

Objectives

Although Lyme disease can be effectively treated with doxycycline, amoxicillin and cefuroxime axetil, discovery of novel drugs will benefit the patients intolerant to these drugs and potentially those suffering from chronic Lyme disease that is refractory to these agents and to macrolides. In this study, we have explored 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase as a drug target for B. burgdorferi, which uniquely possesses three genes expressing homologous enzymes with two of these proteins apparently exported.

Methods

The recombinant B. burgdorferi Bgp and Pfs proteins were first used for the kinetic analysis of enzymatic activity with both substrates and with four inhibitors. We then determined the antispirochaetal activity of these compounds using a novel technique. The method involved detection of the live–dead B. burgdorferi by fluorometric analysis after staining with a fluorescent nucleic acids stain mixture containing Hoechst 33342 and Sytox Green.

Results

Our results indicate that this method can be used for high-throughput screening of novel antimicrobials against bacteria. The inhibitors formycin A and 5'-p-nitrophenythioadenosine particularly affected B. burgdorferi adversely on prolonged treatment.

Conclusions

On the basis of our analysis, we expect that structure-based modification of the inhibitors can be employed to develop highly effective novel antibiotics against Lyme spirochaetes.

 
 
 
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