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Articles by N. Nordin
Total Records ( 3 ) for N. Nordin
  F. Abu Bakar , A.S. Abdulamir , N. Nordin and T.S. Yoke
  Lactobacillus sp. is probiotic bacteria for which many detection methods were envisaged. However, culture-based methods failed to achieve specific detection of this bacterium due to its presence in mixed bacterial complex communities. The PCR assay was optimized to detect and quantify Lactobacillus sp. specifically in complex microbial community of mixed bacteria. Four DNA extraction methods, DNA integrity, primers specificity and optimized PCR procedure were all tested. It was shown that extracted genomic DNA using Wizard® Genomic DNA Purification Kit showed the highest yield, quality and performance in gel electrophoresis. Moreover, the specificity of the primer set, Lacto-16S-F /Lacto-16S-R, specific for Lactobacillus sp. was checked and found highly specific. In conclusion, the best DNA extraction protocol, working specific primer set and working PCR assay were achieved for achieving efficient, specific and reliable molecular-based, culture-independent, method of detection of lactobacillus sp. in PCR-suppressor highly protein-complex environment of mixed bacteria community.
  W.N.N. Wan Omar , N. Nordin , M. Mohamed and N.A.S. Amin
  A two-step biodiesel production from Waste Cooking Oil (WCO) has been optimized. Pre-treatment with ferric sulphate in acid catalysed esterification process preceded transesterification in order to reduce the free fatty acid level in WCO. The objective of this study was to investigate the relationship between reaction temperatures, reaction time and methanol to oil molar ratio in the pre-treatment step. Central Composite Design (CCD) and Response Surface Methodology (RSM) were utilized to determine the best operating condition for the pre-treatment step. The optimum pre-treatment conditions were 60°C, 3 h and 7:1 for reaction temperature, reaction time and methanol to oil molar ratio, respectively with a maximum Fatty Acid Methyl Ester (FAME) yield being 81.3%.
  N. Nordin , M. Idroas , Z. Zakaria , M.N. Ibrahim and A.R. Md. Zin
  This study describes the conceptual design of ultrasonic tomographic instrumentation system for monitoring flaw in pipeline coating. In oil and gas industry, an ultrasonic inspection is the common method used to inspect pipeline integrity due to flaw existence such as corrosion, pitting, holiday, pinhole and others. The ultrasonic tomography system is used in this project to monitor flaws circumferentially on pipeline coating with contactless measurement of distance from sensors which is based on thickness changing in coating. The design of the ultrasonic tomography system consists of ultrasonic sensing system, data acquisition and image reconstruction system. Experimental test for lab scale is performed by using 2 inch pipe. The transceiver sensors of 40 kHz are mounted around the pipe with a distance of 2 cm from sensors to the pipe surface. Reflection mode is used as the ultrasonic sensing mode for the ultrasonic signal as it propagated through air medium to the pipe coating. The data collected are based on ultrasonic signal amplitude and time of flight measured by ultrasonic transceiver sensor. Based on the time travelled by the ultrasonic signal from the sensor to the pipe coating, the distance can be determined using Time of Flight (ToF) method. The thickness changing in pipe coating indicates the existence of flaws (internal or external). From the acquired output data, a tomographic image of pipe coating thickness is reconstructed. In the image reconstruction system, the image coating will be reconstructed using MATLAB software based on suitable algorithm.
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