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Articles by N. Nahar
Total Records ( 7 ) for N. Nahar
  S.M.A. Kawsar , E. Huq , N. Nahar and Y. Ozeki
  Extracts of Macrotyloma uniflorum plants were examined as potential sources of phenolic compounds. Reversed phase high performance liquid chromatography (RP-HPLC) with UV detection was employed for the identification and quantification of the phenolic acids. Eight phenolic acids, namely, 3, 4-dihydroxy benzoic, p-hydroxy benzoic, vanillic, caffeic, p-coumeric, ferulic, syringic and sinapic acids were isolated from an ethanolic extract of Macrotyloma uniflorum. The most abundant phenolic acids were p-coumaric acid (8.95 mg 10-2 g of dry sample) and p-hydroxy benzoic acid (7.81 mg/100 g of dry sample).
  S. M.A. Kawsar , E. Huq , N. Nahar and Y. Ozeki
  S.M.A. Kawsar , G. Mostafa , E. Huq , N. Nahar and Y. Ozeki
  The bioactivity guided separation of the dichloromethane extract of the aerial parts of Macrotyloma uniflorum Linn. resulted in the isolation of methyl ester of hexadecanoic and ethyl ester of hexadecanoic acid mixture (I) and n-hexadecanoic acid (II). The structures of the isolated compounds were elucidated by spectroscopic analysis, including UV, IR, 1H-NMR, 13C-NMR and mass spectroscopy. In addition, the fractionated crude extract of 1-butanol exhibited the significant hemolytic activity by using mouse erythrocytes.
  S.M.A. Kawsar , E. Huq and N. Nahar
  The fractionated crude extracts dichloromethane (CH2Cl2), ethyl acetate (EtOAc), 1-butanol (1-BuOH) and aqueous (H2O) from aerial parts of Macrotyloma uniflorum were screened for cytotoxicity using the brine shrimp lethality bioassay technique. Most of the extracts were found to be non-toxic and this indicates that the ethnobotanical use (oral applications) of the experimental plant are justified.
  S. Islam , M.S. Hossain , N. Nahar , M. Mosihuzzaman and M.I.R. Mamun
  A reverse phase high performance liquid chromatographic method with an acetonitrile-water mobile phase gradient and UV/Visible detection is described for the determination of three pesticides, frequently used in agriculture, in eggplant samples. The samples were sprayed with three pesticides namely, Diazinon, Malathion, Sumithion at three different doses and were harvested at two different Pre-Harvest Interval (PHI) of days 1 and 5 after the application of the pesticides. Sample preparation involved extraction with ethyl acetate and clean up was accomplished by solid-phase extraction using florisil columns. Calibration curves that were constructed for the analytes with matrix matching followed linear relationships with good correlation coefficients (R2>0.990). The average recoveries of the pesticides which were sensitive to matrix effects ranged from 88-120%. Detection limit of less than 0.02 mg kg-1 showed that the method developed can be used to determine the pesticide residues in concentrations lower than the maximum residue limits. In the analysis of residues, samples treated with Diazinon at all doses, residual amounts above Maximum Residue Limit (MRL) (0.02 mg kg-1) were found. Malathion and Sumithion were found above MRL (0.5 mg kg-1) value in only one sample for each pesticide.
  S. M.A. Kawsar , M. Seraj Uddin , E. Huq , N. Nahar and Yasuhiro Ozeki
  In the present research, attempt was taken to explore the antimicrobial potency of the crude extracts of the Macrotyloma uniflorum plant. The extractives of the plant were subjected to screening for inhibition of microbial growth by the disc diffusion method. The zones of inhibition demonstrated by the dichloromethane, ethyl acetate, 1-butanol and aqueous extracts ranged from 11-16, 10-24, 10-14 and 10-12 mm, respectively at a concentration of 500 μg disc-1. The ethyl acetate extract showed promising antibacterial activities against all the gram-positive and gram-negative bacteria whereas dichloromethane extract showed moderate activities and the 1-butanol and aqueous extracts did not show any significant antimicrobial activities. In addition, the antifungal activities of all the extractives were tested, using the food poisoning technique. Only dichloromethane extract has been proved to be active against all fungi tested with a higher inhibition activity than standard nystatin. The overall results provide promising baseline information for the potential use of the crude extracts from M. uniflorum in the treatment of bacterial and fungal infections.
  A.K.M.N. Huda , M.A. Bari , M. Rahman and N. Nahar
  This research programme was undertaken with a view to develop an efficient and reliable method of indirect regeneration through somatic embryogenesis in two varieties of eggplant through the technique of tissue culture. For this purpose, cotyledon pairs of Solanum melongena were used as explants for callus induction. Three different auxins (NAA, 2, 4-D and IAA) were used singly or in combination with BAP for present investigation, as types, concentrations and combinations of growth regulators had marked influence on callus induction. Auxin/cytokinin combinations were proved more potent for callus induction as well as callus growth than that of auxin tried singly in the media. Best callus formation was observed in MS media when supplemented with 0.05 mg L-1 BAP + 2.0 mg L-1 NAA. Best calli from cotyledonary explant were subcultured on MS supplemented with different combinations and concentrations of phytohormones for somatic embryogenesis. Among the different combinations and concentrations 0.05 mg L-1 BAP + 2.0 mg L-1 NAA gave the best result on somatic embryogenesis for Loda variety. On the other hand, somatic embryos of China variety were obtained only on the media having 1.0 mg L-1 BAP + 0.05 mg L-1 GA3. The global structure somatic embryos originated from the superficial layers of the calli, as a single structure or in a cluster. These structures could be isolated easily from parent tissues because they were floated freely in water. These floated free embryos were cultured on nutrient media again to obserb shoot primordia development and thereafter shoot development.
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