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Articles by N. Mohammed
Total Records ( 4 ) for N. Mohammed
  N. Kataria , J. Bargujar , P.K. Pilania , N. Arya , T. Bhati , N. Mohammed , P. Nathawat , M. Mathur , S. Choudhary , S. Asopa , Abhimanu and A.K. Kataria
  To evaluate stress and metabolic adjustments in buffaloes with Post Parturient Haemoglobinuria (PPH), certain serum enzymes viz. Gamma-Glutamayl Transferase (GGT), Glucose-6-Phosphatase (Glc-6-Pase), aldolase, glucose-6-phosphate dehydrogenase, glutamate dehydrogenase, malate dehydrogenase, arginase and 5'nucleotidase enzymes were determined. Simultaneously haemoglobin and serum calcium, phosphorus, glucose and proteins were also determined. Blood samples were collected from adult female healthy and buffaloes with post parturient haemoglobinuria. In affected group the mean values of all the parameters were compared with respective healthy mean values. Significantly (p≤0.05) increased serum GGT activity in buffaloes with PPH indicated stress. Activities of the enzymes related to glycolysis and hexose monophosphate shunt were found to be decreased in affected animals. Activities of other enzymes like Glc-6-Pase, GDH, MDH, ARG and 5'NT were found increased significantly (p≤0.05) in the affected cases. This pattern was suggestive of metabolic adjustments where rate of glycolytic pathway decreased and rate of gluconeogenesis was observed to be increased in the affected cases. Correspondingly, serum glucose concentration of affected animals was significantly (p≤0.05) higher. Serum calcium showed non significant (p>0.05) difference whereas serum phosphorus, proteins and haemoglobin levels decreased significantly (p≤0.05) in affected cases. Decreased levels of phosphorus and disturbed calcium to phosphorus ratio suggested that phosphorous deficiency plays a key role in causing haemoglobinuria in buffaloes. Pattern of variation in the activities of the enzymes related to various metabolic processes revealed the metabolic adjustments. The data obtained in the present investigation support the concept that sustained PPH stress may represent an integrating mechanism underlying metabolic adjustments for homeostasis. Better understanding between PPH stress and metabolic homeostasis can be instrumental in the design of novel therapeutic strategies.
  Taruna Bhati , A.K. Kataria , P. Nathawat , S.K. Sharma , N. Mohammed and M. Mathur
  The present study was undertaken to determine the efficacy of 32 different antibiotics against 38 S. aureus isolates obtained from milk samples from cattle with subclinical mastitis. About 6 antibiotics, i.e., Azithromycin, Gentamycin, Chloramphenicol, Tobramycin, Netillin and Neomycin were found 100% effective followed by Methicillin and Linezolid (94.74%), Cotrimoxazole (92.11%), Nitrofurazone (92.10%), Enrofloxacin (89.47%), Bacitracin (84.21%) and Sparfloxacin, Amoxyclav, Cefaclor and Amoxycillin (81.58%). The resistance was 71.05% for Vancomycin and Polymyxin B, 50% for Oxytetracycline, 47.37% for Azlocillin and 36.84% for Novobiocin whereas Cefixime and Metronidazole were completely (100%) ineffective against all the isolates.
  N. Mohammed , S. Solanki , T. Bhati , P. Nathawat , S.K. Sharma , S. Kumar , A. Joshi , P.K. Pilania , L.N. Sankhala , M. Mathur , A. Ahuja , N. Kataria and A.K. Kataria
  The present study was carried out on 17 positive cases (horses) of colic. Two cases died during the treatment. Ten apparently healthy equines (horses) were also included in the study which constituted control group. Clinico-physiological observations included clinical signs, temperature, pulse, respiratory rate per min and colour of mucosa and were noted daily before start of treatment. Laboratory diagnosis of equine colic was done by determining haematological parameters. Blood samples from control group as well as equines suffering from colic were subjected to haematological examinations comprising of haemoglobin, packed cell volume, total erythrocyte count, total leucocyte count, differential leucocyte count and platelet count. There was great variation in duration and severity of clinical manifestations. In all the cases abdominal pain, loss of appetite, depression and change in colour of mucous membrane from light pink to dark pink was present. Pulse rate, respiration rate and capillary refill time were increased highly significantly (p<0.01) than the mean values from control group but temperature was within normal range and non-significantly (p>0.05) changed. In present study haemoglobin, PCV and TEC counts was increased significantly (p<0.05) on 1st day (before treatment) in comparison to healthy control group and after-treatment group. There was non significant (p>0.05) difference in their values between control group and after treatment group. Pattern of variation observed in the present study regarding haematological parameters of colicky cases can contribute in generating data for future studies and in laboratory diagnosis.
  P.K. Pilania , S. Solanki , N. Mohammed , S. Asopa , R. Maan , A. Joshi , L.N. Sankhala , M. Mathur , M.K. Thori , J.S. Gaur , A. Meena and N. Kataria
  An investigation was carried out to assess oxidative stress in goats of Marwari breed affected from gastrointestinal parasitism and pneumonia belonging to farmers’ stock of arid tract of Rajasthan State, India. The animals were grouped into healthy and affected. All the blood samples were collected in sterile tubes with anticoagulants for erythrocyte separation and determination of erythrocytic Catalase (CAT) and Superoxide Dismutase (SOD) as biomarkers of oxidative stress. Activities of both the enzymes were significantly (p≤0.05) higher in affected animals in comparison to healthy lot. Erythrocytic catalase activity was 2.37 times higher in goats having gastrointestinal parasitism and 3.06 times higher in goats affected with pneumonia. Erythrocytic superoxide dismutase activity was 4.77 times higher in goats having gastrointestinal parasitism and 6.40 times higher in goats affected with pneumonia. It was observed that goats affected with pneumonia showed higher magnitude of enzyme activities in comparison to those having gastrointestinal parasites. Magnitude of change was greater in SOD activities than CAT activities. It can be concluded that gastrointestinal parasites and pneumonia served to produce oxidative stress in the goat. Catalase and superoxide dismutase are considered as potent biomarkers of oxidative stress. They are also named as antioxidant enzymes. Increased activities of both these enzymes signified the presence of oxidative stress in order to combat excessive production of free radicals. Antioxidant supplementation is recommended in cases affected with conditions like parasites and pneumonia to protect the animals from oxidative stress and ensuing damage to cellular machinery.
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