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Articles by N. Mandal
Total Records ( 5 ) for N. Mandal
  S. Gantait , N. Mandal and S. Nandy
  Aromatic plants have been used commercially as spices, natural flavor, raw material for essential-oil industry and other medicinal purpose. Tropical and sub-tropical Asia are rich in the number of aromatic plant species due to their suitable ecological conditions. Micropropagation has superiority over conventional method of propagation because of high multiplication rate but, field performance of these tissue cultured plants depends on the selection of the initial material, media composition, growth regulators, cultivar and environmental factors. Some well developed in vitro techniques are currently available to help growers for meet the demand of the spices and pharmaceutical industry. Identification of somatic clones of plants derived through tissue culture can facilitate commercially viable in vitro propagation for medicinal and aromatic plants. An overview of the regeneration of aromatic plants by in vitro organogenesis from various types of explants is presented in this review article.
  S. Gantait , N. Mandal and P.K. Das
  The genus Allium, consists of hundreds of medicinal plant species, is one of the most imperative sources of life supporting drugs. The in vitro biotechnological interventions are vital to choose, multiply, store up and improve the major Allium sp. In vitro culture of Allium has performed an incredibly crucial role in accelerated growth of several species with desirable traits and production of healthy and disinfectant propagules as well as paved the way towards cultivar improvement. During the last quite a few years, several moves have been made for in vitro propagation of Allium. In vitro regeneration via direct and indirect organogenesis using different explants and plant growth regulator formulations has been comprehensively covered in the literature. Recent challenges for establishment of protocols for genetic transformation have gained preference in the recent past reports. This review article comprehensively describes the exploitation of biotechnology for in vitro regeneration and genetic transformation for enhancement of the genus Allium.
  S. Gantait , N. Mandal , S. Bhattacharyya and P. Kanti Das
  Regeneration efficiency and genetic clonality are two major aspect of successful long-term in vitro culture to be studied extensively. The regeneration efficiency of 25 months sustained in vitro culture of Allium ampeloprassum L. was maintained through multiple shoot proliferation and root growth. No morphological variation was detected between the plantlets regenerated from initial and long-term shoot tip culture or with their mother, meaning a high likelihood of their clonal fidelity. Detection of genetic integrity for in vitro regenerated clones was carried out using 10 ISSR primers among which 4 primers produced a total number of 336 distinct and scorable bands with an average of 7 bands per primers where the other 6 primers were not reproducible. The ISSR primers base on AG motif and 3' anchoring produced more number of consistent bands. All monomorphic bands in the ISSR assay both for primary culture as well long term culture in vitro ascertained to a great extent their genetic integrity through their partial genetic coverage.
  S. Gantait , N. Mandal , S. Bhattacharyya and P.K. Das
  Aloe (Aloe vera L., Family Liliaceae), with proven multiple medicinal values finds favour readily in Ayurevdic applications, is facing a serious threat to its population as well as biodiversity due to its popularly harvested aloe leaves by the local communities and herbal medicine vendors. To succeed in dealing with the specified problem along with the facilitation of the mass production of commercial level, constant supply of quality propagules can be possible through conservation of propagules in vitro. The present study is thus concerned with in vitro conservation of multiple shoot culture of aloe to achieve unbroken supply of propagules maintaining their genetic purity. Rhizomatous stem explants result multiple bud break in MS plus 0.25 mg L-1 of NAA and 1.5 mg L-1 of BAP. Separated shoot buds further result in shoot multiplication and proliferation on MS with 2.5 mg L-1 of BAP. In vitro generated multiple shoots were split into individual shoot and subcultured for further multiplication in a sustainable. Five subcultures were performed at an extended 5 month interval over a period of 25 months in the same medium. Plantlets regenerated after 1st subculture and plantlets from 5th subculture showed no significant difference in the phenotypic response. Genetic integrity of in vitro clones was tested using ISSR primers. All monomorphic bands in the ISSR assay, both for primary culture as well long term culture, ascertained their genetic integrity to a great extent.
  A. Das and N. Mandal
  Stevia rebaudiana Bert. is an economically important medicinal plants act as a sugar substitute for diabetic and obese people. In the present investigation a novel protocol was developed to accelerate the production of embryogenic calli so that large number of in vitro plant regeneration can be feasible. Two different types of amino acids (Trypthphan and Glutamine) in different concentration and three different types of additives (yeast extract, casein hydrolysate and Potato extract) were utilized along with MS medium supplemented with 2,4-D (2.0 mg L-1), BAP (0.5 mg L-1), sucrose (30 g L-1) and agar with leaf as explant to elucidate their role in developing and maintaining embryogenic calli. It was found that glutamine (50 mg L-1) and casein hydrolysate (100 mg L-1) produced greenish, healthy nodular calli with more embryogenic potential and less necrotic lesion in comparison with only PGR supplemented basal medium which further performed better in regeneration medium for producing large number of regenerated shoots.
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