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Articles by N. Jayabalan
Total Records ( 3 ) for N. Jayabalan
  M. Ganesan , P. Bhanumathi , K. Ganesh Kumari , A. Lakshmi Prabha , Pill-Soon Song and N. Jayabalan
 

Problem statement: The present investigation described a simple and reproducible protocol for transgenic cotton regeneration and characterization of chitinase (Chi II) gene expression against two different fungal pathogens in cotton.
Approach:
Transgenic cotton (Gossypium hirsutum cv. SVPR2) plants were produced by pCambia-bar-Chi II (13.8 kb) under the control of the CaMV 35S promoter, harbored in the strain LBA 4404 Agrobacterium tumefaciens by using shoot tip explants.
Results: Finally, from the 10 experiments, 21.8% of transformation frequency was recorded. Segregation ratio of 3:1 was recorded in the T0 plant seeds. Polymerase chain reaction and southern blotting analysis were used to confirm the integration of Chi II transgene in the T0 plants genome of putative transgenics. Quantitiave and qualitative (SDS-PAGE) analyses were also carried out to confirm the expression of chitinase enzyme in T0 plants. Further, randomly selected transgenic plants (T0) were analyzed for disease tolerance by evaluating them with spores of Fusarium oxysporum and Alternaria macrospora. All the selected PCR positive plants showed enhanced disease resistance against Fusarium wilt. The plants selected randomly showed an enhanced survival rate compared with the control when they were grown in earthen pots inoculated with 1x105 spores 100-1 g of soil mixture. Another four randomly selected plantlets were sprayed with spores of Alternaria macrospora in order to test their tolerance to Alternaria leaf spot disease. After 20 days of culture, the number of lesions per leaf and the lesion length per leaf spot in non-transferred leaves increased. In the case of transgenic plantlets, lesion formation was completely absent.
Conclusion: The disease resistance against Fusarium wilt and Alternaria leaf spot in cotton strains would serve as good breeding materials for producing fungal disease resistant cotton varieties.

  S. Vinoth , P. Rajesh Kanna , P. Gurusaravanan and N. Jayabalan
  Indigofera trita L.F. SPP. subulata (vahl ex poir) distributed in the southern part of India particularly in Tamilnadu and it has potential medicinal properties and used in the treatment of tumours and liver disorders. The work carried out in the plant is much less, the present work was designed to investigate the preliminary phytochemical, antibacterial and GCMS analysis of various parts of the plant. The medicinally compounds from crude extracts of leaf, stem and root portions were fractionated in different solvents (aqueous, chloroform, petroleum ether and ethanol) subjected to preliminary phytochemical and antibacterial activities. The potential extracts were analysed through FTIR and GCMS. Phytochemical screening of leaves, stem and root extracts of Indigofera subulata revealed the presence of alkaloids, Quinones, reducing sugars, saponins, terpenoids and tannins. The ethanol extract of leaves and stem was found to exhibit activity against Pseudomonas aeruginosa Fourteen compounds were identified by GC-MS analysis. Phytomedicines avenues for the identifications of medicinally significant compounds with potential activity and it will lead to the isolation of potential antibiotics. Thus Indigofera subulata can be used as multi resistant drug in future.
  S. Vinod Kanna and N. Jayabalan
  Direct organogenesis from leaf explant of Indian variety of Solanum melongena L. (PLR1) was successfully achieved. Eggplant leaves cultured for 10–12 days on MS medium supplemented with (2iP) 2.0 mg L–1 and Naphthalene acetic acid (NAA) 1.0 mg L–1 induced high frequency shoot organogenesis (79-81%) and favored shoot elongation. Shoots developed from leaf explant directly after two weeks of explant inoculation. Shoot proliferation reached maximum in the presence of 2.0 mg L–1 2iP with induction of 4.5-5.0 average mean number of shoots per explant. High frequency of root induction (83-85%) was observed on medium supplemented with 1.0 mg L–1 Naphthalene acetic acid (NAA) with 6-7 mean number of roots whereas increase in the level of (IBA) Indole butyric acid (2.0 mg L–1) resulted in induction of roots along with development of callus from the base of the shoots. The plantlets with developed roots are hardened with 2:1 ratio of soil and vermiculite shifted to controlled environmental growth chamber with 8 h photoperiod for acclimatization and after ten days the plantlets are transferred to field condition. This protocol can be efficiently used for mass multiplication and also for regeneration of genetically transformed Solanum melongena tissue.
 
 
 
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