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Articles by N. Ghaemi
Total Records ( 3 ) for N. Ghaemi
  A. Gholamhoseinian , N. Ghaemi and F. Rahimi
  Catalase (EC. oxidoreductase) has been found in all aerobic organisms. Most of the work preformed on this enzyme obtained from mammalian, bacterial and fungal sources as there is less information about plant catalases. Partial purification of catalase from Brassica oleracea capitata (Cabbage) and its kinetics was studied. To this intention, freshly harvested cabbage leaves freezed in liquid nitrogen, reduced to small pieces and blended. The extraction with 0.1 M Na2 HPO4 buffer solutions have performed. The filtrate after centrifugation half-saturated with solid Am-Sulfate (A.S) then 35% saturated with solid A.S. After the partially purified enzyme dialyzed, the extract was eluted from a sephadex G-200 column equilibrated with phosphate buffer. The enzymatic activity was observed in only one peak. The optimal pH of the cabbage leaf catalase was 7-8. When the concentration of stabilized catalase increased, the reaction rate increased concomitantly. The substrate was not inhibitory to the reaction rate up to 0.1 M of H2O2 concentration. In this study Vmax and Km of cabbage leaf catalase was 31.12 μM min-1 and 25.5 mM, respectively.
  A. Keyvan , S. Moini , N. Ghaemi , A.A. Haghdoost , S. Jalili and M. Pourkabir
  The study was designed to investigate the effect of duration of frozen storage on chemical analysis, lipid damage and extractability of Myofibrillar proteins of Kutum (Rutilus frisi kutum. The fish were collected from Anzaly landings in north of Iran and were subjected to 12 months of frozen storage and analyzed at intervals of three month. Protein content ranges from 21.8±0.01 to 19.9±0.01. Protein decrease with increasing duration of frozen storage; fish samples that were for frozen thirty days; having highest protein content 21.8±0.01 while the least 19.9±0.01 was recorded for fish samples that were frozen for 12 months. Similar results obtained for the fat content where the highest fat content 3.21±0.01 was recorded for the fish samples that were for frozen 30 days and the least value was recorded for those stored for 12 months. The least moisture content was observed for fish samples the was stored for 12 months but, the highest Ash content was observed for fish samples the was stored for 12 month. Lipid damage were measured on the basis of Free Fatty Acids (FFA), Peroxide Value (PV), Thiobarbituric acid index (TBA-i). PV, TBARS and FFA concentration of frozen Caspian Sea white fish stored at -18°C; the temporal variation of these three variables were statistically significant (p<0.001). SDS-PAGE patterns showed that myosin heavy chain was much more susceptible to hydrolysis than actin.
  H. Zamanian , M. Mehdipour and N. Ghaemi
  Psalmocharias alhageos is an important pest of vine in most parts of Iran, Afghanistan, Pakistan, southern areas of Russia, Turkey and Iraq. This cicada is spread in most provinces in Iran such as Esfahan, Hamedan, Qazvin, Markazi, Lorestan, Qom, Kerman, Tehran and Kordestan. In addition to vine, this insect damages some other fruit trees, such as apple, sour cherry, quince, peach, pomegranate and pear trees and some non-fruit trees, namely white poplar, ash, elm, eglantine, silk and black poplar trees. The nymphs of cicada damage the trees by feeding on root, adult insects on young bud and by oviposition under branch barks. Nourishing root by nymph leads to the weakness of the tree and hinder its growth. The high density oviposition of adult insects inside young barks causes withering of branches. The resulted damage on vine products is 40% which is one of the most important factors in product reduction in vineyard. This research was conducted in Takestan in Qazvin. It was conducted for the first time to study the behaviors of the mates of this vine cicada in order to manage it. Two systems were used to record the sound of male cicada called analog voice-recorder and digital voice recorder. To analyze the recorded sound of the male cicada we used of spectrum analyzer, digital storage oscilloscope and protens 7 computer softwares. We could call the attention of natural enemies an disturb the male insect`s attracting sound by producing natural and artificial sound in the range of 1-6 kHz in two different ripeness status of the fruits and could prevent mating and oviposition of female cicadas.
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