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Articles by N. Choudhary
Total Records ( 2 ) for N. Choudhary
  N. Choudhary , U. Ahuja , V. Chawla , R.K. Jain , P. Kumari and K.R. Batan
  Weedy rices commonly known as ‘red rices’, infest rice fields worldwide. They compete with rice for nutrients, deteriorate quality and are difficult to eradicate. Understanding their genetic diversity and the origin may prove helpful in designing an effective way for managing them. A collection of weedy rices from Haryana were evaluated along with cultivated and wild rices for variability and diversity at morphological and molecular level using 35 ISSR primers. Significant variation was observed at morphological and genetic level in weedy rices of Haryana. Weedy rices were found to be distinct from white and red type cultivated rices and wild strains. Twenty one ISSR markers gave amplification of DNA and a total of 91 polymorphic bands were found. With 9 ISSR markers 20 unique bands were observed in 17 genotypes. Markers such as Pr814, Pr836 and Pr858 produced unique bands in weedy rices only. Alleles unique to weedy rices observed by ISSR markers could be converted into SCAR markers for identification. Weedy rices were placed alone as well as with other groups in cluster analysis. Both short and long grain weedy rices were found in collection though at present only long grain types are cultivated indicating that leftover progenies of local cultivars segregated into various plant types giving rise to the weedy rice.
  S. Asthana , R. Sharma , N. Choudhary , P.A. Vyas , M.A. Rather and G. Krishna
  Cytogenetic characterization plays an important role in cataloguing and conservation of germplasm. Osteobrama belangeri is one of the important fish species in North East India and need urgent attention for conservation. Hence cytogenetic characterization was carried out for Osteobrama belangeri. Samples of the specimen were collected from Manipur, India and maintained in wet laboratory in live condition. Metaphase chromosome preparation was made using standard hypotonic solution, methanol acetic-acid fixation followed by Giemsa staining for karyotyping of the species. The diploid chromosome number was found to be 50 and the karyotype is composed of 14m+8sm+28 (st and t) with fundamental arm No. (FN) 72. Positive chromosome bands like C, G and NOR bands were developed on the metaphase chromosome spread. NOR bands were found on the constriction regions near centromeric, as well as, slightly away from terminal regions. While, G bands was at A, T-rich regions appearing to be away from centromere towards the end of the chromosome bodies. C bands were localized on the regions adjacent or immediate to the centromeres and on the constrictions as indicating the concurrency with constitutive hetrochromatin. The result showed the diploid chromosome number as 50 which is mostly seen in other cyprindis and the study could localize C, G and NOR bands for the first time on this species.
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