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Articles by N. Anuar
Total Records ( 4 ) for N. Anuar
  A.K.M.A. Islam , N. Anuar and Z. Yaakob
  The objective of the study was to explore the effects of pre-sowing seed treatments on germination behaviour and to assess the possibilities of increasing the germination rate of Jatropha curcas. Seeds of twenty jatropha accessions obtained from seven different sources were subjected to three pre-sowing treatments viz., control (T0): unsoaked seeds directly sown in the polybag and apply water up to saturation; T1: seed placed on filter paper in the petridis and moistened once with the water; and T2: seeds kept under stone sand and moistened once with the water. Seeds in T1 and T2 were kept for 72 h before sown in the polybag. The study was conducted in the Glass House of Plant Biotechnology Laboratory, Universiti Kebangsaan Malaysia. Study revealed that pre-sowing treatments significantly (p<0.01) enhanced seed germination parameters of Jatropha. Seed germination started 5 days after sowing and continued up to 12 days. The highest germination percentage (95.85%) was observed in T2 and 100% germination was observed in the genotypes viz., UKM-JC-011, UKM-JC-012, UKM-JC-014, UKM-JC-016 and UKM-JC-020 in T1 and T2. None of the genotypes showed 100% germination in T0. The highest Germination Index (GI) and Seedling Vigor Index (SVI) was found in T2 and the lowest in T0. T2 was found more effective in respect to faster germination, high germination percentage, germination index, seedling vigor index, speed and energy of germination. Five accessions viz., UKM-JC-012, UKM-JC-014, UKM-JC-016, UKM-JC-017 and UKM-JC-019 were found suitable in all the treatments including control.
  N. Saifudin , N. Ibrahim and N. Anuar
  The aim for this study was to develop recombinant Chinese Hamster Ovary (CHO) cell line producing high and stable β-galactosidase by using Lipofectamine and TransFast as transfection systems. A comparison of these two gene delivery systems was made using β-galactosidase protein expression as the endpoint readout. Parameters such as lipid to DNA ratios and different amounts of Lipofectamine or DNA used were determined for optimization of transfection. In the Lipofectamine system the highest clone number was obtained from the combination of 4.5 μL Lipofectamine and 0.75 μg DNA or 2:1 charge ratio. By using TransFast, a lipid to DNA charge ratio of 2:1 is suitable for transfection. Clone TF 9 (7) that was transfected with 9 μL TransFast and 1.5 μg DNA but maintaining this 2:1 charge ratio was found to be the most productive clone in β-galactosidase production even when scaled up to 100 mL in zeocin free medium. The highest level of production for this clone and other productive clones are on the fourth day of cultivation. The highest β-galactosidase activity by subclone TF9 (7) was about 9.64 U mg-1 in 100 mL working volume of zeocin free medium. This study showed TransFast was more efficient than the Lipofectamine LTX for transfection and lipid mediated DNA delivery is an efficient mean for LacZ gene transfer into CHO cell.
  S. Za`imah Syed Jaapar , M.S. Kalil and N. Anuar
  Photo fermentation is a biological process that can be applied for hydrogen production. The process is environmental friendly which is operated under mild conditions using renewable resources. In order to increase yield of H2 produced by Rhodobacter sphaeroides, some experimental factors that may enhance H2 production were studied. The effect of operating parameters including agitation, aeration and light on hydrogen production using R. sphaeroides NCIMB 8253 was investigated. Rhodobacter sphaeroides NCIMB 8253 was grown in 100 mL serum bottle containing growth medium with maliec acid as the sole organic carbon source. The cultures were incubated anaerobically at 30°C with tungsten lamp (100 W) as the light source (3.8 klux) and argon gas was purged for maintaining anaerobic condition. The results show that maximum hydrogen produced was higher (54.37 mL) in static culture with 69.98% of H2 in the total gas compared with shake culture (11.57 mL) with 57.86% of H2. By using static culture, H2 produced was five times higher compared with non-static in both aerobic and anaerobic condition. It was found that growth and H2 production with fluorescent lamp showed better results than growth and H2 production with tungsten light.
  A.F. Almansoory , M. Idris , S.R.S. Abdullah and N. Anuar
  In this study both plants Ludwigia ectovolvis and Scirpus mucronatus have been shown to phytoremediate by toxicity testing using various concentrations of gasoline. Plants grow in different ratios comprising of garden soil and sand before executing the toxicity tests. Soil mixture as garden soil to sand ratios (25:75, 50: 50, 75:25, 100% sand and 100% garden soil) to propagation the plants for 56 days. The results shown that L. ectovolvis could grow and survive in mixture of garden soil to sand ratio of 50:50, while for S. mucronatus the best favorable growth was using 75: 25 ratio of garden soil to sand. S. mucronatus survived the gasoline concentrations of 10, 20, 30, 40, 50, 60, 70, 90 and 100 g gasoline/kg in comparison to the control. However L. ectovolvis died after one day of exposure at similar concentrations, therefore the plants were tested for lower concentrations of 1, 2, 3, 5 and 7 g kg-1. During 14 days of exposure, S. mucronatus can only survive at concentrations of 10, 20, 30 and 40 g gasoline/kg. But L. ectovolvis withered under concentration of 1, 2, 3, 5 g gasoline/kg at the end of exposure period. As a conclusion, S. mucronatus has the potential to remediate hydrocarbon contaminated soil as compared to L. aectovolvis.
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