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Articles by N. Al-Hmoud
Total Records ( 3 ) for N. Al-Hmoud
  N. Al-Hmoud , H. Al-Rousan , B.O. Hayek and M.A. Ibrahim
  The objective of this study was to survey for the genetically modified maize and soybean food products in the Jordanian market. The study was designed to extract genomic DNA of maize and soybean products by cetyltrimethylammonium bromide (CTAB) method and to identify specific genes for maize and soybean, expression control specific genes 35S promoter and NOS terminator by polymerase chain reaction analysis. For confirmation test of the genetically modified maize and soybean food products, nested polymerase chain reaction experiments were performed using internal primers for the detection of the E35S promoter and the hsp70 exon1/intron1 region of maize MON810 and Cp4 EPSPS gene of soybean. Three out of 19 maize food products were identified as carrying amplified DNA fragments of 35S promoter region, the nested PCR test confirmed the presence of MON810 event. One of three soybean food products was identified as carrier DNA fragments of 35S promoter region, Cp4 EPSPS event was not detected.
  A. Makkiya , M.A. Ibrahim , N. Al-Hmoud , H.H. Hasani and H.M. Said
  Acute Myeloid Leukemia (AML) is the most frequent cause of acute leukemia affecting adults, its incidence increases steadily with age and has been correlated with DNA methylation aberration and inactivation of tumor suppressor genes. Recent epigenomic studies showed that DNA methylation abnormalities have been observed in age related acute myeloid leukemia and indicated the importance of DNA methylation analysis for AML diagnosis. An insight of the connection between DNA methylation and AML might help in development of novel molecular diagnostic and genomic therapies. Epigenetic drugs of two families namely the DNA-demethylating agents and inhibitors of histone deacetylase have emerged as the most promising compounds in this area and several pharmaceutical compounds have received approval for the treatment of specific leukaemia and lymphoma subtypes. In addition possible combination between molecular therapeutic approaches including the activation of certain signal transduction pathway(s) like the interleukins family and chromatin-remodeling events is feasible by the application of epigenetic drugs. This approach might be one of the potential promising solutions for the AML treatment. The present study reviewed recent advances in the research related to genomic and epigenomic of acute myeloid leukemia.
  Mohammed A. Ibrahim , N. Saleh , Khalida M. Mousawy , N. Al-Hmoud , E. Archoukieh , Haithum W. Al-Obaide and Mohannad M. Al-Obaidi
  In this study the molecular genomic polymorphism of age related acute myeloid leukemia was analyzed by twenty one arbitrary primers of decamer oligonucleotides to investigate the genetic polymorphisms. Two categories of RAPD primers were identified, according to their ability to amplify genomic DNA. Thirteen primers were found unable to amplify genomic DNA of acute myeloid leukemia patients. On the other hand, eight primers were able to amplify genomic DNA and were divided into two subgroups, first group showed monomorphic DNA fragments, whereas the second one gave polymorphic bands. One of the polymorphic amplifying primers showed unique patterns of amplified DNA fragments in the genomic DNA of age related acute myeloid leukemia.
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