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Articles by N. Sadeghifard
Total Records ( 5 ) for N. Sadeghifard
  N. Sadeghifard , M.M. Aslani and A. Ghasemi
  The aim of the present study was to compare four laboratory methods for diagnosis of Helicobacter pylori. 101 sets of four antral biopsies were collected from 101 patients, one of the biopsies in each set were tested by rapid urease test, other three biopsies were used for culture, direct staining and histopathology, respectively. By culture method in 62 cases (61.3%), Helicobacter pylori were isolated. Among several primary media that tested in this study, Brucella agar supplemented with 10% whole sheep blood supported relatively good growth of H. pylori. In present study 65 cases (64.3%) were positive by rapid urease test. Of 101 biopsy specimens in 68 cases (67.3%) were obtained positive result by histopathology method (Gimsa Staining). Sensitivity and specificity of direct staining test were 89.7 and 96.9%, respectively. We found histopathology method was the best method for diagnosis of H. pylori and it can be selected as gold standard in detection of Helicobacter pylori.
  M.H. Shirazi , R. Ranjbar , S. Eshraghi , G. Sadeghi , N. Jonaidi , N. Bazzaz , M. Izadi and N. Sadeghifard
  In the present study, the in vitro inhibitory effects of G. glabra extract against the growth of Salmonella typhi, S. paratyphi B, Shigella sonnei, S. flexneri and enterotoxigenic E. coli (ETEC E. coli) was investigated using well and disc diffusion method. Shigella spp. and enterotoxigenic E. coli but Salmonella paratyphiB showed no susceptibility to liquorice with concentrations lower than 7.5%, however all tested bacterial strains exhibited susceptibility to high concentration of liquorice. Results obtained from present study showed that G. glabra can be considered as an alternative herbal antibacterial agent against the bacterial strains tested.
  N. Sadeghifard , M.H. Salari , M.R. Ghasemi and F. Amin-Harati
  The aim of this study was to compare stool cytotoxin assay as the standard test with enzyme immunoassay system (C. difficile Tox A/B Tech Lab) and C. difficile bacterial culture on cycloserine-cefoxitin fructose agar followed by cytotoxin assay (toxigenic culture). A total of 650 stool specimens from hospitalized patients with nosocomial diarrhoea were collected from December 2002 to February 2004. C. difficile was detected by culture method in 146 samples. Of these samples 103 isolates were toxigenic by toxigenic culture. One hundred twelve and 108 stool specimens were positive for C. difficile toxins by enzyme immunoassay (Tox A/B immunoassay) and stool cytotoxin assay, respectively. Ninety eight samples were diagnosed to be positive in all test methods. Toxigenic C. difficile was isolated from 2 stool cytotoxin assay and 3 enzyme immunoassay negative stools. Sensitivity and specificity for Tox A/B immunoassay in relation to the stool cytotoxin assay was 95.6% (95% CI) and 98.3% (95% CI), respectively. The results of the present study showed that sensitivity and specificity Tox A/B immunoassay was very close with stool cytotoxin assay.
  M.H. Shirazi , N. Sadeghifard , R. Ranjbar , E. Daneshyar and A. Ghasemi
  Incidence of asymptomatic bacteriuria during pregnancy among Iranian women was examined. Midstream urine was collected from 380 pregnant women and streaked on blood agar and incubated for 24 to 48 h. Growth was considered significant if 105 mL-1 bacteria were present. Among the pregnant women, 10.1% had asymptomatic bacteriuria. Age, past history of abortion, proteinuria, level of education, number of fertility had no significant association with asymptomatic bacteriuria occurrence. But lower socioeconomic status, leucocytosis and a history of UTI were associated with an increased incidence of asymptomatic bacteriuria (p<0.05). The most frequently isolated pathogen was Escherichia coli (68.4%), followed by Staphylococcus epidermidis (10.5%)) and Klebsiella pneumonia (8.3%). According to the results, co-trimoxazole was the best antibiotic; followed by nitrofurantoin which 60.5 and 44.7% of strains were sensitive to these two antibiotics, respectively.
  A. Rostamzad , N. Sadeghifard and A. Ahmadi
  In this study, laboratory routine tests were compared with adenosine deaminase (ADA) test for diagnosis of tuberculous pericarditis. Sampling was performed among 30 patients admitted to two Iranian teaching hospitals (Imam and Modarres). For each patient two specimens were obtained from the pericardial fluid and biopsy. Pericardial fluid was used for staining, culture and measuring of ADA activity. The biopsy specimens were homogenized and cultured on Lowenstein-jensen media too and examined by H & E stain for presence of caseous granulomas and tubercule bacilli. In this survey, the ADA activity level of less than 45 μL-1 in pericardial fluid were determined as negative, while more than 45 μL-1 were considered as positive reaction indicating of tuberculous pericarditis. In this study, from a total of 30 subjects, tuberculous pericarditis was diagnosed by routine laboratory tests in 13 patients who all had clinical symptoms of the disease. Tuberculous pericarditis was diagnosed by positive results of pericardial fluid cultures in 6 of 13 patients (46.2%), by pericardial biopsy cultures in 6 patients (46.2%), by pericardial fluid staining in 3 patients (23.1%) and by tuberculin skin test in 10 patients (76.9%). Finally we observed that all of 13 patients with tuberculous pericarditis had ADA levels of more than 45 μL-1 in their pericardial fluids. The results of the present study confirmed that high levels of ADA have a prognostic value and due to its high specificity and sensitivity and also being faster and easier than laboratory routine tests, so this test can utilized as effective diagnostic method for diagnosis of tuberculous pericarditis.
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