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Articles by N. Iqbal
Total Records ( 3 ) for N. Iqbal
  M. Aslam , M. T. Elahi and N. Iqbal
  Attempts were made to incorporate intraploid interpsecific gene(s) from Gossyium barbadense into G. hirsutum through DNA-mediated embryo transformation approach. G. barbadense DNA was injected into the styles/ovaries of G. hirsutum, 24 hours after self pollination. Two phenotypic types of plants i.e. transformed and G. hirsutum like were observed in D population. The transformed plants had clearcut variations in boll weight, yield and other quality traits confirmed to recipient parent. D transformations were also heritable and were transmitted to D generation. The transformed plants had better boll weight (4.9 gm), better fibre fineness (3.9 μg/in) and better fibre strength (101.0 TPPSI) compared to the boll weight (3.0 gms), fibre fineness (4.5 μg/in) and fibre strength (91.0 TPPSI) of G. hirsutum. The transformation efficiency for quantitative traits was about 20 % where as low for qualitative traits like flower petals with red spot (0.08 %). In the present studies transformed genotypes illustrated higher yield potential and better fibre quality than the recipient (G. hirsutum) parent.
  N. Iqbal and S. Farooq
  Isozyme analyses were used to detect inter- and intraspecific variation in different accessions of wild rice species, 0. officinallis and 0. punctata. Some F1 hybrids between cultivated and wild rice species were also utilized. Fresh leaf extracts were electrophoresed on polyacrylamide gels. Different isozymes viz., Esterases, Glutamate oxaloacetate transaminases and peroxidases were used as biochemical markers. On the basis of isozyme banding profiles, distinct variations were observed between and within the two wild rice species and their different accessions. Based on polymorphic isozyme profiles, hybrids of rice cultivars, Basmati 198 x 0. officinalis, Nonabokra x 0. malampuzhaensis ace: 100957 and EF-6 x 0. malampuzhaensis ace: 100957 were also identified. Our study demonstrated that isozyme markers can successfully be used for detection of genetic diversity as well as for the identification of hybrids.
  N. Iqbal , S.M. Reader , P.D.S. Caligari and T.E. Miller
  Previously reported genomic in situ hybridization protocols failed to differentiate Aegilops uniaristata chromosomes in a polyploid wheat background. Different protocols and hybridization temperatures were tested to optimize the differentiation of N genome A. uniaristata chromosomes from those of the A, B and D genomes of wheat. A combination of pre-hybridization of cytological preparations, pre-annealing of the genomic probe with blocking DNA and hybridization at elevated temperatures proved successful. A genomic in situ hybridization protocol for the differentiation of chromosomes from very closely related genomes in a polyploid background is reported.
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