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Articles by Mustafa ULUKANLIGIL
Total Records ( 2 ) for Mustafa ULUKANLIGIL
  Mehmet TANYUKSEL , Mustafa ULUKANLIGIL , Hasan YILMAZ , Zeynep GUCLU , R. Engin ARAZ , Gurkan MERT , Ozgur KORU and William A. PETRI, Jr
 

Aim: The spectrum of clinical intestinal disease due to Entamoeba histolyticainfection ranges from an asymptomatic carrier state to fulminant colitis with an array of manifestations. The extent of strain diversity for E. histolytica and the extent to which strains differ in virulence are not clearly known. In this study, we aimed to investigate the genetic diversity of E. histolytica isolates from Turkey.

Materials and Methods: In order to study genetic diversity, the serine-rich gene of E. histolytica (SREHP) was investigated in 26 clinical isolates from Turkey by nested PCR amplification and restriction enzyme fragment length polymorphism (RFLP) analysis.

Results: Twelve distinct DNA patterns were observed after AluI digestion of nested PCR products. The results demonstrate an extensive genetic variability among Turkish E. histolyticaclinical isolates.

Conclusions: Genotyping seems to be a useful epidemiologic tool to identify common-source outbreaks as well as strain-specific manifestation of an infection.

  Mustafa KARAOGLANOGLU , Omer Faruk AKINCI , Mustafa ULUKANLIGIL , Melike Rusen METIN , Huseyin CETIN and Nurdan CAY
  Aim: To perform an in vitro study of the effects of various scolicidal agents on scolices in both crystal-clear fluid and daughter cysts. Materials and methods: A 2-staged procedure, using 15% NaCl, 3% NaCl, Betadine, 1% NaOCl, and alcohol, was performed on the cysts. Into bottles containing 10 cysts, 20 mL of each agent, including crystal-clear fluid, was added. A single daughter cyst was then taken from the bottles after 5, 10, 15, 20, 40, 60, and 120 min and the injected samples were examined microscopically for viability using activity and eosin staining. In the second stage of the study, the direct effects of the scolicidal agents on the scolices were investigated at 1, 5, 10, 15, 20, 40, and 60 min. Results: The scolices became inactive and were stained after 1 and 2 min in 15% NaCl and povidone iodine, respectively. The scolices stained completely after 20 min with 3% NaCl, while 1% NaOCl affected the scolices at 5 min. On the other hand, alcohol affected them at 65 min. Scolices in the control group remained viable for 36 h. In the second stage, scolices in the control group were inactivated at 30 min. Complete staining was observed at 5 min with 15% NaCl and at 20 min with 3% NaCl, as in Betadine solution. It was found that 1% NaOCl immediately inactivated the scolices. Lastly, with alcohol, scolices were stained completely at 8 min. Conclusion: It was seen that 1% NaOCl, 15% hypertonic saline, and povidone iodine solutions were effective scolicidal agents against the scolices, both in the crystal-clear fluid and the daughter cysts. On the other hand, 3% hypertonic saline was not effective enough as a scolicidal agent. Alcohol was ineffective, especially on the scolices in the daughter cysts.
 
 
 
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