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Articles by Munti Yuhana
Total Records ( 8 ) for Munti Yuhana
  Dewi Nurhayati , Widanarni and Munti Yuhana
  The experiment was conducted to examine the effects of dietary administration of probiotic SKT-b (Vibrio alginolyticus ) and oligosaccharide from sweet potato ( Ipomoea batatas L. ) on growth performance and immune responses of white shrimp (Litopenaeus vannamei). Shrimp (0.33±0.02 g) was fed with basal diet (control without challenge test (-) and control with challenge test (+)), supplemented with combination of probiotic and prebiotic (probiotic 0.5% + prebiotic 1% (A), probiotic 1% + prebiotic 2% (B), probiotic 2% + prebiotic 4% (C)) for 30 days of culture. After feeding experiment, five shrimp per tank were sampled for bacterial quantification and immunity determination. Then all the shrimp rest were challenged by infectious myonecrosis virus and V. harveyi. After 30 days, Daily Growth Rate (DGR) and Food Conversion Ratio (FCR) of the shrimp were significantly better in treatment B than the control but weren’t significantly different (p>0.05) in other treatment. Other than that, phenoloxidase (PO) activity and Total Haemocyte Count (THC) of the shrimp in all synbiotic treatments were higher from the control group. After co-infection challenged test, PO, THC and Respiratory Burst (RB) activity of the shrimp in all synbiotic treatments were higher from control (+). Besides that, lowest survival shrimp occurred in the control (+) was significantly different compared with all synbiotic treatments.
  Maulid Wahid Yusuf , Nur Bambang Priyo Utomo , Munti Yuhana and Widanarni
  This study was conducted to study growth performance of catfish (Clarias gariepinus) in biofloc-based super intensive culture added Bacillus sp. cells addition. Biofloc cultured in separated aquarium (100 L) was used as source of inocula for cultivation for C. gariepinus (average body weight of 2.3±0.12 g fish–1). Different concentration of Bacillus sp., cells was included in diets and in the media, i.e., (A) 102 CFU mL–1, (B) 104 CFU mL–1, (C) 106 CFU mL–1, biofloc without Bacillus sp., cells addition (K+), without biofloc as well as Bacillus sp., cells addition (K‾, control). Results showed that the Survival Rate (SR) of catfish showed significantly different between Bacillus sp., cells addition and control (p<0.05). Growth and feed conversion ratios in Bacillus sp., cells addition were better than that of the controls. Nutrient content of biofloc containing 106 CFU mL–1 showed the highest protein value of 34.06%. Total bacterial cells abundance were ranging from 106-108 CFU mL–1 cells, with or without the addition of Bacillus sp., as a control. The highest performance of catfish growth was obtained using biofloc treatment by 106 CFU mL–1 Bacillus sp., addition compare to other treatment.
  Hessy Novita , Iman Rusmana , Munti Yuhana and Fachriyan Hasmi Pasaribu
  Motile Aeromonad Septicaemia (MAS) disease in catfish (Clarias gariepinus) is caused by infection with Aeromonas hydrophila. One alternative way to control infection by this bacteria is applying probiotics, which inhibit quorum sensing, also known as Anti-Quorum Sensing (AQS), using Bacillus sp. administered by feeding. Bacillus sp. product AHL (Acyl Homoserine Lactone) Lactonase, which is speculated to be able to inhibit quorum sensing in pathogenic bacteria. In vivo test of probiotics was set up in triplicate and consisted of five treatments. Effect of AQS probiotic bacteria as feed supplement was set up in improving survival rate, Feed Conversion Ratio (FCR), Specific Growth Rate (SGR) and non specific immune response of catfish (Clarias gariepinus). The result showed that the fish feed with AQS had a high percentage of survival rate. The AQS mix of treatment (TS1RifR, TS2RifR and TA23RifR) had the highest survival rate (93%). The AQS treatments were also had values of FCR and SGR better than that of control. However, the control treatments had survival rate only 31%. The AQS isolates could also improve a non specific immune response. Result indicated that AHLs degrading bacteria could be considered as better alternative to replace application of antibiotics in aquaculture as biocontrol of bacterial fish disease and in reducing the pathogenicity of A. hydrophila in catfish (Clarias gariepinus).
  Dwi Febrianti , Munti Yuhana and Widanarni
  White Spot Syndrome Virus (WSSV) is one of the most devastating shrimp pathogen which causes up to 70-90% mortality in commercial shrimp farming. This study was aimed to evaluate the effectiveness of synbiotic microcapsule (Bacillus NP5 RfR and mannan oligosaccharide combination) feed supplementation on the immune responses and growth performance of WSSV infected Pacific white shrimp. Shrimps (4.411±0.395 g) were cultured and fed by pellet containing synbiotic microcapsule at different dosages for 30 days, i.e., designated as 0.5% (M1), 1% (M2), 2% (M3) (w/w) and without synbiotic microcapsule addition (negative and positive controls). The synbiotic was encapsulated by spray drying method. Challenge test was performed at 31st of cultivation day by performing intramuscular injection of WSSV filtrate (104 copies mL–1). Compared with control, the shrimp fed by M2 and M3 were found to have higher (p<0.05) value of Total Hemocyte Count (THC), pro Phenol Oxidase (proPO) and Respiratory Burst (RB) activity compared to positive control. The results showed that the Specific Growth Rate (SGR) of M1 and M2 were higher (p<0.05) than controls. Yet, the Feed Conversion Ratio (FCR) were lower (p<0.05) than controls. Above all, the survival rate of shrimps fed with microcapsule synbiotic were higher (p<0.05) than controls.
  Rifqi Tamamdusturi , Widanarni and Munti Yuhana
  A feeding trial was conducted to investigate the effects of administration of microencapsulated probiotic Bacillus sp. NP5 and prebiotic mannan oligosaccharide (MOS) for the prevention of Aeromonas hydrophila infection on Pangasianodon hypophthalmus. Pangasius with initial body weight of 6.54±0.17 g were stocked into 40 L aquariums with a stocking density of 10 fish per aquarium. Control (C) diet (no addition of Bacillus sp. NP5 and MOS), probiotic Bacillus sp. NP5 1% (10 g kg–1), prebiotic MOS 0.2% (2 g kg–1) and synbiotic (probiotic Bacillus sp. NP5 1%+prebiotic MOS 0.2%). Then 10 fish of each aquarium were challenged by Aeromonas hydrophila. The results showed that fish with synbiotic supplementation resulted the highest Specific Growth Rate (SGR), Food Conversion Ratio (FCR) and those were significantly different (p<0.05) from the other treatment. The immune responses showed that fish fed with the control diet resulted the lowest hematocrit, hemoglobin and red blood cell count and those were significantly different from the probiotic and prebiotic group (p<0.05). White blood cell count, phagocytic activity and respiratory burst activity of the fish fed with probiotic 1% and MOS 0.2% were not significantly different from control. Moreover, fish fed with diet supplemented with probiotic, prebiotic and synbiotic had notably lower mortality after 10 days infected with A. hydrophila (p<0.05). Dietary Bacillus sp. NP5 and MOS had a significant interaction on enhancing immune responses and growth performances of Pangasianodon hypophthalmus (p<0.05).
  Waode Munaeni , Disnawati , Munti Yuhana , Mia Setiawati , Asis Bujang , La Ode Baytul Abidin and Ardana Kurniaji
  Background and Objective: Vannamei shrimp Litopenaeus vannamei is an economically valuable aquaculture commodity but still faces problems due to disease and growth performance. Buton forest onion extract Eleutherine bulbosa (Mill.) Urbhas been shown can protect the shrimp from disease. This study aimed to evaluate the potential of the Buton forest onion extract on the growth of vannamei shrimp in tarpaulin pond. Materials and Methods: The experiments were conducted for 90 days by adding Buton forest onion extract in feed at a dosage of 40 g kg1. The Buton forest onion bulbs was extracted by maceration method using 96% ethanol. The test feed was made through the re-pelleting method. Treatment include supplementation of Buton forest onion extract diet supplemented with Buton forest onion extract diet at twice a week (treatment A) and control was without Buton forest onion extract(treatment K). Parameter measurement include final weight, average daily growth (ADG), feed conversion ratio (FCR), survival rate and water quality (temperature, salinity, turbidity and pH). Results: This study was demonstrated the addition of Buton forest onion extract on feed with a frequency twice a week in significantly improve the growth performance of vannamei shrimp by improving the final weight and average daily growth at 65 and 90 days of maintenance. Water quality during the study was still within the optimum range for L. vannamei shrimp growth. Conclusion: This study showed that supplementation of Buton forest onion extract was able to improve the growth performance of shrimp in tarpaulin ponds.
  Lukman Anugrah Agung , Widanarni and Munti Yuhana
  Streptococcus agalactiae is one of the fish pathogen which can cause mortality up to 90% in commercial tilapia farms. This study aimed to evaluate the effectiveness of supplementation of micro-encapsulated probiotic (Bacillus NP5), prebiotic (MOS) and combination of those materials (synbiotic) through the feed on growth performances and immune responses of tilapia infected with S. agalactiae. The probiotic cells were encapsulated by spray drying method. The experimental fish were reared for 40 days and fed with feed-supplemented with probiotic, prebiotic and synbiotic and without any supplementation to the feed (positive and negative control). On day 40, all fish except negative control were challenged by S. agalactiae via intraperitoneal route injection in amount of 0.1 mL (106 CFU mL–1). This study showed that administration of 0.4% prebiotic to the feed resulted the best growth performance in the end of feeding trial and survival rate after the challenge test with S. agalactiae.
  Hamsah , Widanarni , Alimuddin , Munti Yuhana and M. Zairin Junior
  Background and Objective: The application of probiotics, prebiotics and synbiotics is commonly applied in juvenile and adult shrimp administered through artificial feed, while in larvae, it has still been limited. This study aimed to evaluate growth performance, the total intestinal bacteria, the activity of enzymes and the survival rate of Pacific white shrimp (Litopenaeus vannamei) administered Pseudoalteromonas piscicida 1Ub, mannan-oligosaccharides and synbiotic (the combination of P. piscicida 1Ub and mannan-oligosaccharides) through bio-encapsulation of Artemia sp. Materials and Methods: Bio-encapsulation of Artemia sp. was done by adding P. piscicida 1Ub 106 CFU mL–1, mannan-oligosaccharides 12 mg L–1 and synbiotic (P. piscicida 1Ub 106 CFU mL–1 and mannan-oligosaccharides 12 mg L–1) to the rearing medium of Artemia sp., for 4 h. The administration of the enriched Artemia sp. to the shrimp larvae was done from mysis 3 to Post Larvae (PL) 12. The body length and the body weight of Pacific white shrimp larvae were observed at the beginning and the end of the study, while RNA/DNA ratio, the activity of enzymes, survival rate and total bacteria of shrimp larvae were analyzed at the end of the study. Results: The results showed that daily growth rate, absolute length, RNA/DNA ratio, the activity of enzymes, survival rate and total bacteria of shrimp larvae administered probiotic, prebiotic and synbiotic were higher (p<0.05) than the control. The synbiotic treatment gave the best results in daily growth rate (24.39±0.31%), absolute length (13.00±0.50 mm), RNA/DNA ratio (0.6369±0.0094 μg mL–1), the activity of enzymes (protease 0.033±0.0007, lipase 0.047±0.0010, amylase 0.853±0.008, mannanase 0.148±0.004 U mL–1 min–1), survival rate (92.67±1.26%) and total bacteria (6.7×107 CFU larvae–1). Conclusion: The administration of P. piscicida 1Ub, mannan-oligosaccharides and synbiotic through bio-encapsulation of Artemia sp., effectively improved the growth performance of Pacific white shrimp larvae with the best results demonstrated by the synbiotic treatment.
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