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Articles by Muna O. Elhaj
Total Records ( 3 ) for Muna O. Elhaj
  Abbas Mohamed Ahmed , N.T. Mekki , Muna O. Elhaj and Sarah Mohamed Abusalab
  Forty batches of anthrax spore vaccine were produced by bioreactor and by Roux flask, 20 batches were obtained from each system. The number of doses produced in the flask system was found 10 times more than those obtained by the bioreactor. It is recommended to continue producing this vaccine by the Roux flask while research will go on to improve suitable conditions in the fermenter in order to obtain high doses and to avoid the drawbacks of the flask system.
  Muna O. Elhaj and S.M. ElSanousi
  Local Sudanese isolates of S. aureus ssp. anaerobius (which were isolated from sheep abscesses in the qurantene and slaughter house for exported sheep) were compared with the reference strain of S. aureus ssp. anaerobius on the genetic level by detection of the Restriction Fragment Length Polymorphism (RFLP) by Pulsed-Field Gel Electrophoresis (PFGE). PFGE involves embedding organisms in agarose, lysing the restriction endonucleases that cleave infrequently. Slices of agarose containing DNA fragments are inserted into the wells of an agarose gel and the restriction fragments are resolved into a pattern of discrete bands in the gel by an apparatus that switches the direction of current according to a predetermined pattern. The DNA restriction patterns of the isolates are then compared with one another to determine their relation. PFGE of S. aureus ssp. anaerobius local Sudanese isolates determined that all local isolates were identical, regarding the RFLP, whereas the reference strain have two bands at different locations, but it considered as same strain.
  Muna O. Elhaj and S.M. Elsanousi
  Six local Sudanese isolates of S. aureus subsp. anaerobius which were isolated from sheep abscesses were compared with the reference strain of S. aureus subsp. anaerobius ATCC 35844 DSM (Deutsche Sammlung von Microorganismen und Zelkulturen, Braunschweig, Germany) No. 20714 in their protein profiles to choose the candidate local isolate for vaccine production.Vertical electrophoresis was used to separate protein of cell lysates and supernatants of the local Sudanese isolates and the reference strain of S. aureus subsp. anaerobius. It was found that the electrophoretic protein pattern for all local isolates and the reference strain resembles each others to a greater extent and many bands are common in all strains. Cell lysates of all local isolates (except isolate 5) and the reference strain had protein bands ranged between 19-24 with molecular mass ranging from 53.35-113.84 KDa. Isolate 5 have 17 protein bands with molecular mass ranging from 53.62-74.89 KDa. All local isolates and the reference strain had no protein bands in their supernatants, except isolate 2 which has 5 protein bands in its supernatant. Most of the local isolates are the same as the reference strain, two local isolates have minor differences compared to the reference strain.
 
 
 
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