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To study inheritance of CLCuV, nine selected varieties/lines (four resistant five susceptible) of up land cotton (G. hirsutum L.) were used for five F1 combinations. The F1 was sown for raising F2 and back crossing purpose. Subsequently P1, P2 F1, F2, BC1 and BC2 population were established and to ensure the inoculation of CLCuV no pesticide was used to control insect population. The results indicated that duplicate dominant epistasis was involved in control of resistance of CLCuV. Virus resistance was controlled by two dominant duplicate genes as F2 ratio was modified to 15:I from 9:4:4:1 and the test cross ratio was modified to 3:1 instead of 1:1.