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Articles by Mukesh Srivastava
Total Records ( 5 ) for Mukesh Srivastava
  Deepti Singh , Amit Kumar Verma , Amit Kumar , Mukesh Srivastava , Shanker Kumar Singh , Arvind Kumar Tripathi , Ashish Srivastava and Iftekhar Ahmed
  The early detection of the Canine Parvo Virus (CPV) is of paramount importance. The present study was aimed to know the molecular epidemiology of Canine parvo virus. Canine faecal samples from 100 dogs showing the clinical signs of gastroenteritis in and around Mathura, Uttar Pradesh, India were collected and DNA was extracted by phenol-chloroform method. CPV vaccine strain was used as a positive control. Polymerase Chain Reaction (PCR) was carried out to amplify VPI/VP2 gene using a set of 20-mer primers [pCPV-RT (Forward): 5’-CAT TGG GCT TAC CAC CAT TT-3’; (Reverse): 5’-CCA ACC TCA GCT GGT CTC AT-3’)] from position 3136-3155 to 3276-3295 of VP1/VP2 gene. A PCR product of approximately 160 bp was generated with positive faecal samples and CPV vaccine strain. After screening, 63 dogs were found positive for CPV but no sex variation was noticed amongst the CPV positive cases. Dogs, of the age group of ≤6 months were more susceptible in comparison to of >6 months and highest occurrence was noted in unvaccinated dogs and dogs in co-habitation with other dogs. Breed wise distribution of CPV in dogs revealed that the prevalence of CPV was the highest in Doberman (77.78%), followed by Spitz (78.57%), German shepherd (70.00%), Labrador (68.75%), Pomeranian (45.45%). It is concluded that CPV is prevalent in the Mathura and nearby area and it is more common in pups of age less than 6 months old and more prevalent in German shepherd, Labrador and Pomeranian breeds of dog.
  Rajesh Kumar , A.K. Verma , Amit Kumar , Mukesh Srivastava and H.P. Lal
  With the increasing trends of pet ownership the chances of campylobacteriosis are also increasing as these pets are kept in close visicinity of owners. The prevalence and antimicrobial sensitivity profiles of Campylobacter isolates from faeces of dogs attended in veterinary practice at Teaching Veterinary Clinical Complex, Mathura, India. During the period of investigation (October 2009 to April 2010), 100 rectal swabs from dogs were collected and transported to the laboratory for further investigations. Bacteriological examination revealed 51.00% prevalence rate of Campylobacter isolates in dogs faecal samples. The disc-diffusion method was used to know the susceptibility of all the 51 Campylobacter isolates against 10 commonly used antimicrobials in pet animal practice. High rates of resistance were observed to erythromycin (90.20%), tetracycline (88.23%), ampi-cloxacillin (88.23%), ciprofloxacin (80.39%), enrofloxacin (68.63%) and aymoxycillin-clavulanic acid (19.61%). All the Campylobacter isolates were susceptible to amikacin, levofloxacin and streptomycin. Erythromycin and ciprofloxacin are drugs for treatment of human campylobacteriosis. The high resistance rate to these drugs among Campylobacter isolates from dog faeces is of public health significance as dogs are supposed to be the main source of infection in human beings.
  Rajesh Kumar , A.K. Verma , Amit Kumar , Mukesh Srivastava and H.P. Lal
  Campylobacteriosis is one of the leading causes of gastroenteritis in humans and various researches suggested that owning a pet is a risk factor for the disease. To determine the prevalence and risk indicators for Campylobacter sp. infecting dogs attending veterinary practice at TVCC, DUVASU, Mathura, 100 dogs with and without the clinical symptoms of diarrhoea were examined and the prevalence of Campylobacter sp. was 51.0%. Breed wise prevalence showed that nondescript (73.68%) dogs were more likely to carry Campylobacter sp. A significant difference in isolation rates was observed between younger and older dogs: 56.58% of the younger dogs (≤ 1 year) were positive, compared with 33.33% of adult dogs (> 1 year) (p<0.01) as seen at the veterinary University, Mathura, India. Dogs sharing a household with another dog, dogs that had not received antibiotic treatment in the previous months and the age of the dog were significant indicators of Campylobacter carriage. Recent diarrhoea or vomiting in dogs with Campylobacter, breed, sex or vaccination status were not statistically significant. The high prevalence of Campylobacter in puppies supports the hypothesis that dogs, particularly young ones shed Campylobacter spp., which can be of impact for public health.
  Mukesh Srivastava , Rajeev Vasantrao Gaikwad , Abdul Samad , Barkha Sharma and Ashish Srivastava
  Glomerular Filtration Rate (GFR) is considered as the best single parameter for assessing renal function because it is directly proportional to the number of functioning nephrons. Serum creatinine is the most frequently measured analyze in clinical biochemistry as an indirect indicator of glomerular Filtration Rate (GFR). Renal scintigraphy has been used as direct measurement of the glomerular Filtration Rate (GFR) either of individual kidney or global (both kidneys). By this technique, the GFR is calculated by a regression equation relating the percent of the injected dose of radiopharmaceutical, technetium-99m Diethylene-Triaminepentaacetic acid (DTPA), taken up and filtered by the kidneys. This communication reports the relationship of direct (scintigraphy) and indirect (creatinine) methods of GFR measurement. Scintigraphy is a quick, noninvasive diagnostic technique in which a two-dimensional picture of internal body tissue is produced through the detection of the gamma radiation emitted by radioactive substances injected into the body within 15 min with a gamma camera. Serum creatinine increases in renal failure correlating with a decrease in GFR forming a curvilinear relationship. Based on the study undertaken in 44 dogs with renal failure, it was found that 86.36% dogs suffering from renal failure showed a conventional pattern of curvilinear relationship between creatinine and GFR. Inter-individual variations were observed in 11.36% of dogs. False positive results were obtained in 9% of total dogs, where creatinine was within normal reference range but GFR decreased on scintigraphic analysis. False negative observations were seen in 4.5% of dogs, where normal GFR with marginally high creatinine values was reported.
  Sanjhi Paliwal , Vikrant Sudan , Daya Shanker and Mukesh Srivastava
  Background and Objective: Knowledge of local isolates and strains is a prerequisite for development of both effective mass vaccination strategy and a suitable molecular marker based diagnostic tool. The pathogenesis of bovine tropical theileriosis (BTT) caused by Theileria annulata in susceptible ruminants is known to vary depending upon the isolate and strain involved. The present study was designed to characterize T. annulata isolates from Northern India using PCR-RFLP technique. Materials and Methods: In the present study, HSP 70 gene was amplified from 28 naturally infected calves found in BTT endemic semi arid zone of Northern India. The amplified product was then digested with two restrictions enzymes viz., Taq I and Alu I to find out the pattern of restriction digests so as to have an idea of various strains of T. annulata involved. Results: With Taq I enzyme, two restriction digestion products were noticed with molecular weights of 100 and 175 bp, respectively. So far as Alu I enzyme is concerned, three restriction digestion products with molecular weights of 60, 90 and 125 bp, respectively, were noticed. Conclusion: Monomorphic pattern of restriction digest was noticed in all 28 studied T. annulata isolates, for both the enzymes, suggesting of presence of single strain of parasite involved in the studied area.
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