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Articles by Muhammad Zia
Total Records ( 2 ) for Muhammad Zia
  Sara Kayani , Muhammad Zia , Sadia Sarwar , Riaz -ur-Rehman and M. Fayyaz Chaudhary
  With the objective to promote in vitro callus induction, leaf segments of Achyranthes aspera were inoculated on basal MS medium supplemented with 3.0% sucrose and 0.8% agar with different concentrations of 2,4-D alone and in combination with NAA, BAP, IAA, IBA and Zeatin. The explants were maintained in growth room at 25 ±1 °C and 16 h light cycle. The best callus induction was obtained with 2,4-D (1.0 and 2.0 mg L-1) in combination with NAA (0.5 mg L-1). Callus induction and good texture from leaf explant was also observed at 2,4-D with BAP. On these combinations morphologically, light green, soft, compact and non-embryogenic callus (Type III callus) was observed. While morphology of callus and callogenic response was poor at 2,4-D alone or in combination with other hormones at different concentrations.
  Saima Malik , Muhammad Zia , Riaz-ur-Rehman and M. Fayyaz Chaudhary
  The study was conducted to analyze the effect of plant growth regulators on callogenesis and direct and indirect organogenesis of Memordica charantia. Callus cultures were induced from leaf, stem and cotyledonary explants of Momordica charantia, at different auxin and cytokinin concentrations either in single or in combination in MS medium. The best callogenic response was observed from all three explants (leaf, stem and cotyledon) on MS medium supplemented with 1.0 and 1.5 mg L‾1 BAP with 1.5 mg L‾1 NAA and 1.0 mg L‾1 2,4-D, respectively. The callus produced was hard, green and compact. These totipotent cells were failed to give rise shooting response when transferred to same or different growth regulator containing medium as second subculture. Indirect organogenesis response was very low or absent due to hardening of callus and habitutation. Best shooting was observed at 1.0 mg L‾1 BAP + 0.1 TDZ and 1.5 mg L‾1 BAP + 0.2 mg L‾1 NAA from shoot tip and cotyledonary node explants. While root formation was achieved when generated shoots were transferred to MS medium both full and half strength supplemented with different auxin concentrations.
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