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Articles by Muhammad Saeed
Total Records ( 11 ) for Muhammad Saeed
  Muhammad Tahir Abbas , Muhammad Arif , Muhammad Saeed , M. Reyad-ul-ferdous , Muhammad Adeel Hassan , M.A. Arain and Abdur Rehman
  A trial was conducted to evaluate the effect of fat emulsifier on fat utilization in broiler chickens using 240 days old chicks which were divided into 24 replicates in a 3×2 factorial arrangement under Completely Randomized Design (CRD). Experimental diets were formulated using 1, 2 or 3% fat with or without fat emulsifier (Lecithin) at 350 mg kg–1. The trial lasted for 35 days and was divided into starter and finisher phases. Statistical analysis of the data collected during the trial revealed that feed intake was not affected (p>0.05) by fat and fat emulsifier. Increasing fat levels resulted in a linear decrease (p<0.05) in starter weight gain but it didn’t affect (p>0.05) finisher and overall weight gain. Fat emulsifier affected (p<0.05) finisher, final and overall weight gain but starter weight gain remained unaffected (p>0.05). Starter Feed Conversion Ratio (FCR) increased (p<0.05) with increasing fat levels but finisher and overall FCR remained unaltered (p>0.05). Fat emulsifier didn’t affect (p>0.05) starter FCR but finisher FCR was improved with fat emulsifier supplementation (p<0.05). Dietary fat didn’t affect (p>0.05) Dry Matter (DM) and Ether Extract (EE) digestibility but fat emulsifier significantly improved (p<0.05) DM and EE digestibility. The carcass composition study results revealed that fat emulsifier affected (p<0.05) the heart weight and linearly increasing fat levels resulted in a linear decrease (p<0.05) in gizzard weight. The combination of fat and fat emulsifier affected (p<0.05) finisher FCR, finisher weight gain, DM digestibility and EE digestibility. This investigation reveals that dietary fat should be avoided in starter diets and supplementation of fat emulsifier in broiler diets may improve DM and EE digestibility.
  Jan Muhammad Shah , Taufique Ahmed Qureshi , Tahmina Sayed , Qurban Ali Shah , Ilahi Bux Kalhoro , Muhammad Asif Arain , Muhammad Saeed , Farman Ali Siyal and Zohaib Ahmed Bhutto
  Objective: The aim of current study was to evaluate the therapeutic and high doses of florfenicol (FFC) on hematological values in goats. Methodology: Goats were intramuscularly induced with therapeutic (20 mg kg –1 b.wt.) and high doses (40 and 60 mg kg –1 b.wt.) of florfenicol for 3 days with 24 h interval. The blood samples were drawn at different timings from 0-120 h after drug administration and were assayed for the selected hematological parameters. Results: The therapeutic dose of FFC produced no effect on erythrocytes, hemoglobin, packed cell volume and leukocytes indices at all timing points, whereas decreased the lymphocyte count (p<0.05) at 24 h. The high dosages of FFC produced significant effect on erythrocytes, hemoglobin, packed cell volume, leukocytes and lymphocyte indices. In comparison with control, erythrocytic count and hemoglobin level (p<0.05) decreased from 48-72 h. Packed cell volume was observed to be reduced (p<0.05 and p<0.01) for 72 h. The leukocytic count (p<0.05) diminished from 24-72 h. The lymphocytic count decreased (p<0.05 and p<0.01) for 96 h. Clinically, local discomfort, reactions and pain at site of injection and some digestion disturbance were also noticed with high doses of florfenicol during experiment. Conclusion: It was concluded that both the therapeutic and high doses of florfenicol significantly affects the lymphocytes. The high doses of FFC produced anemic and immunosuppressive effects of drug.
  Zaib Ur Rehman , Ahsan Ul Haq , Naasra Akram , Mohamed E. Abd El-Hack , Muhammad Saeed , Shahid Ur Rehman , Chunchun Meng , Mahmoud Alagawany , Maryam Sayab , Kuldeep Dhama and Chan Ding
  Objective: The present study was performed to determine the influence of dietary Acetic Acid (AA) supplementation on growth performance, intestinal histomorphology, blood hematology and serum constituents of broilers. Methodology: A total 200 chicks were randomly divided into five experimental groups with four replicates each (10 chicks/replicate) in a complete randomized design experiment. Treatments were as follow: (AA0: control, AA1: basal diet+0.1% acetic acid kg–1, AA2: basal diet+0.2% acetic acid kg–1 and AA3: basal diet+0.3% acetic acid kg–1). Results: The results showed that AA supplementation improved weight gain (p<0.001) and feed conversion ratio (p<0.001). Generally, acetic acid supplementation at 0.3% level improved bird’s performance during 2-6 weeks of age. Increased intestinal length and higher intestinal weight were recorded in AA treated birds. Significant reduction in pH of proventriculus and ventriculus (p<0.01) was observed with in dose related manner. No significant effects were observed on dressing percentage. Histological observations revealed that intestinal morphology professed positive effects under AA treatment. Furthermore, the effect of dietary AA supplementation was significant (p<0.05) only on lymphocytes count and heterophil/lymphocyte ratio. It is obvious that blood of birds fed AA3 diet had the highest concentration of calcium, phosphorous, total protein and globulin comparing with the control diet and other levels of AA. The best results of all parameters were observed in AA3. Conclusion: Based on the results, it could be concluded that AA supplementations in feed employs positive effect on performance and intestinal histomorphology of broilers.
  Muhammad Saeed , Mohamed E. Abd El-Hack , Mahmoud Alagawany , Muhammad A. Arain , Muhammad Arif , Muhammad A. Mirza , Muhammad Naveed , Sun Chao , Muhammad Sarwar , Maryam Sayab and Kuldeep Dhama
  Chicory (Cichorium intybus) is a perennial herbal plant of the dandelion family Asteraceae, usually with bright blue flowers, rarely pink or white. Several varieties are cultivated for salad leaves, chicons or roots which are baked, ground and used as a coffee substitute and supplement. It is also grown as a forage plant for poultry and animal. In addition, chicory herb plays a key role as antioxidant, anti-inflammatory, sedative, immunological, productive and reproductive enhancer, cardiovascular, hypolipidemic, anticancer, anti-protozoal, gastroprotective, antidiabetic, analgesic, anthelmintic, antimicrobial, wound healing and bitter tonic without inducing therapeutic adverse effect. Regarding the hepatoprotective activity, chicory extract reduced the levels of hepatic enzymes such as alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP). Also, chicory plant is a good and very important protective source for hepatocytes and other liver cells as well as it is used as prebiotic against some species of pathogenic bacteria for both in vitro and in vivo. Moreover, it enhances immunity and feed efficiency by decreasing pathogenic microorganisms of gastrointestinal tract. Cichorium intybus roots also were used for the relief of mild digestive disorders, such as feeling of flatulence, abdominal fullness, temporary loss of appetite and slow digestion. The present study highlights the importance of chicory as a feed additive used to improve growth and productive performance of poultry as well as salient beneficial applications in animals and humans. Furthermore, it explains the mechanisms of action underlying the beneficial effects of chicory and to find the effective level in poultry that would act as liver tonic.
  Muhammad Saeed , Xu Yatao , Zaib Ur Rehman , Muhammad Asif Arain , Rab Nawaz Soomro , Mohamed E. Abd El-Hack , Zohaib Ahmed Bhutto , Benazir Abbasi , Kuldeep Dhama , Muhammad Sarwar and Sun Chao
  Non-digestible oligosaccharides as well as phenolic compounds inulins and fructooligosaccharides of Smallanthus sonchifolius make it an attractive functional food. Consumption of these non-digestible oligosaccharides improves gastrointestinal metabolism, increases the growth of bifido bacteria in the colon and also acts as antioxidant, antimicrobial, prebiotic, growth promoter, hypoglycemic, hepatoprotective compounds via lowering alanine aminotransferase (ALT) and increasing mineral absorption to maintain bone homeostasis and also help to reduce cholesterol and triglyceride levels. Past to present literature have been reviewed and the results indicated that Smallanthus sonchifolius root consumption modulates the immune system by regulating the intestinal secretion of IgA and interferon IFN-γ subsequently enhancing resistance to infections and allergic reactions. This role not only facilitates the exclusion of potential pathogenic bacteria but also ultimately increases defense of the host. Previously published literature has focused on rat, human, pig and livestock but biological and clinical evidence is scarce on the same aspect in poultry. Therefore, this review article aimed to highlight the origin, chemical composition, different beneficial uses and biohazards of yacon plant (which contains 70-80% fructooligosaccharides (FOS)) that could be used as a novel natural prebiotic in poultry feed. The FOS could improve different health aspects and productive performance criteria of different poultry species. To recommend yacon plant as a natural and safe medicinal agent, more indices need to be determined in further studies on different livestock and poultry species on molecular level to assure its benefits and to give accurate recommendation for the optimal levels to be added as feed additive.
  Muhammad Saeed , Mohamed Ezzat Abd El-Hack , Mahmoud Alagawany , Muhammad Naveed , Muhammad Asif Arain , Muhammad Arif , Rab Nawaz Soomro , MohibUllah Kakar , Robina Manzoor , Ruchi Tiwari , Rekha Khandia , Ashok Munjal , Kumaragurubaran Karthik , Kuldeep Dhama , Hafiz Muhammad Nasir Iqbal and Chao Sun
  Green tea is an important herb and its products are extensively used in the traditional Chinese medicinal system through various formulations. Several reports have revealed its beneficial usage and medicinal aspects for various ailments. Consumption of green tea has gained special attention and popularity in various sectors of the modern era of changing lifestyle. This review aimed to extend the current knowledge on the modes of action and beneficial applications of green tea in humans and animals for safeguarding different health issues. The nutritional, immunological, pharmacological and physiological functionalities of green tea are because of the available bioactive components like caffeine, L-theanine, polyphenols/flavonoids and others. It possesses multi-beneficial potential in treating and preventing various disorders of animals and humans, as well as promoting animal (dairy, piggery and goatry) and poultry industry. The present review provides insights on the modes of action and beneficial applications of green tea, which will be useful for nutritionists, pharmacists, physiologists, researchers, veterinarians and animal and poultry producers. Future research emphasis and promotional avenues are needed to explore its potential therapeutic applications for designing appropriate pharmaceuticals, complementary medicines and effective drugs to popularize and propagate its multidimensional health benefits.
  Li Chang Xing , Dhanasikar Santhi , Abdul Ghaffar Shar , Muhammad Saeed , Muhammad Asif Arain , Akhtar Hussain Shar , Zohaib Ahmed Bhutto , Mohib Ullah Kakar , Robina Manzoor , Mohamed E. Abd El-Hack , Mahmoud Alagawany , Kuldeep Dhama and Mo Chen ling
  Medicinal plants have gained great popularity and their importance is realized world over. Psyllium (Plantago ovata) is a native plant of India. The hydrophilic mucilloid, water-soluble fiber derived from psyllium husk and seed had been used since ancient times as vital curative agent for constipation, mainly as a part of the traditional Indian Ayurvedic medicine. Psyllium is a rich source of fiber and has numerous other therapeutic impacts such as cholesterol lowering, antidiarrhoeal, laxative, antidiabetic, energy increasing, hemorrhoid remedy, soothing inflammation and weight loss agent. Psyllium husk plays a key role in lowering serum cholesterol, so psyllium is being considered as a potential supportive agent in the therapy of hyperlipidemia. The cholesterol level is lowered in different animal models by the binding of psyllium husk with bile acids in the small intestine and thus reducing its absorption. The husk of psyllium could be used as an additive in milk replacer of the neonatal dairy calves to improve the physiological functions and enhance the performance and health status. Psyllium may also has positive impacts in growth and productive performance of different poultry species. The main aim of this review was to discuss the role of psyllium in managing the hypercholesterolemia in humans and the application of psyllium huskas a safe feed additive in poultry farming for the production of organic and low cholesterol designer egg and meat.
  M. Shafi Nazir , M. Naveed Afzal , Abdul Jabbar , Muhammad Saeed and Shah Nawaz
  Intercropping of methra between 4-, 6-, 8- and 10-row strips of wheat decreased wheat grain yield by 26.92, 22.98, 18.96 and 15.48% respectively compared to wheat alone. However, at the cost of this much reduction in wheat grain yield, additional harvests of 740, 549.5, 382 and 241.5 kg ha–1 of methra from 4-, 6-, 8- and 10-row wheat strips, respectively, were obtained which compensated more than the losses in wheat production. Among the various wheat-methra intercropping systems, the highest net income of Rs. 19463 ha 1 with benefit cost ratio of 5.10 was obtained from wheat grown in 100 cm-spaced 4 cm-row strips + 3 rows of methra, while minimum net income of Rs. 11723 ha–1 with benefit cost ratio of 3.78 from sole cropping of methra.
  Tariq Mahmood and Muhammad Saeed
  Nitrogen level and plant density effects on different agro-physiological traits of maize cv., "Golden" were studies under field conditions at the Agriculture Research Area, University of Agriculture, Faisalabad in Autumn 1996. Treatments comprised four nitrogen levels, i.e., 0, 100, 200 and 300 kg N ha–1 and three plant densities viz., 11.11, 8.33 and 6.67 plants m–2. Yield components, such as number of cobs per plant, number of grains per cob and 1000-grain weight were decreased at higher plant density and low nitrogen levels. The maximum grain yield of 7.11 t ha–1 was recorded from the crop fertilized at 300 kg N ha–1 and raised at 11.11 plants m–2, while the minimum (2.03 t ha–1) was found in control (plant density of 6.67 plants in without N fertilization). The results suggest that 200-300 kg N ha–1 along with 11.11 plants m–2 should be used to obtain the maximum grain yield to maize cv. "Golden" under the edaphic and climate conditions matching to those of the experiment.
  Hafiz Muhammad Akram , Muhammad Sarfraz Iqbal , Muhammad Saeed , Allah Yar , Abbas Ali , K.A. Sahi and Mushtaq A. Nadeem
  To nose out the best drought tolerant wheat variety, an experiment was conducted during 1998-99 at Agronomic Research Farm, Ayub Agricultural Research Institute, Faisalabad, Pakistan. Ten wheat genotypes namely, Inqilab-91, Chakwal-83, Pasban-90, 85205, Rohtas-90, 4072, 4943 and Faisalabad-83 grown under an iron structure with moveable plastic sheet cover for protecting the plants from rain, were exposed to soil moisture stress at milking stage. Stress was imposed simply by withholding one irrigation. Various physiological and agronomic attributes were studied. On the basis of absolute grain yield, cultivar Barani-83 was found to be the most drought tolerant because of its better osmotic adjustment and Inqilab-91 the most sensitive one as it suffered from maximum loss (37.38 %) in grain yield due to decline in 1000- grain weight.
  Kashaf Imran , Muhammad Saeed , Muhammad Atif Randhawa and Hafiz Rizwan Sharif
  Phytochemicals has got special attention of researchers because of their value in food preservation and value addition due to consumer preference. Grapefruit (Citrus paradisi) is one of important member of family Rutaceae (citrus family). Oil was extracted through solvent extraction method using soxhlet’s apparatus. The nuggets were prepared by incorporating the grapefruit peel oil at different levels 0 (control), 5, 10, 25, 50, 100% and stored for duration period (0, 7 and 14 days). Peel oil refractive index, specific gravity, pH, acid value, iodine values 1.47, 0.85, 3.67, 0.81, 99.4 were respectively calculated. The peak values for proximate analysis (protein, moisture, fat, ash contents) were 62.32, 15.29, 14.0 and 2.76 respectively were recorded in T1 (5% grapefruit peel oil) at all storage periods. In color, water activity and texture analysis were 117.77, 0.94 and 1691.7 respectively in T1 at all storage durations. Similarly in sensory evaluation of color, taste, flavor and overall acceptability found statistically that T3 (25% grapefruit oil) treatment is highly significant at all storage periods and gave maximum value for these parameters and T5 (100% grapefruit oil) gave minimum value for its effectiveness. Grapefruit peel oil was checked for its antimicrobial activity test e.g., total plate count, disc diffusion method and minimum inhibitory action against Escherichia coli on chicken nuggets. In antimicrobial analysis MIC (Minimum Inhibitory Concentration) decreased with increasing the concentration of grapefruit peel oil and in disc diffusion method, its values increased with increasing grapefruit peel oil.
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