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Articles by Muhammad Maher
Total Records ( 2 ) for Muhammad Maher
  Gamal Enan , Khalid A. Shaaban , Ahmed Askora and Muhammad Maher
  This research described a preliminary studies for controlling two Escherichia coli strains (E. coli W1 and E. coli W2) isolated from polluted water by their novel coliphages which identified herein and designated ECP1, ECP2 and ECP3. E. coli W1 and E. coli W2 strains were isolated from polluted water and identified using usual cultural, Morphological and Biochemical Methods. Three bacteriophages lysing E. coli strains were isolated. The bacteriophage lysing E. coli W1 (coliphage1) was designated ECP1 while coliphage2, coliphage3 were highly specific to infect E. coli W2 and consequently have been designated ECP2, ECP3, respectively. Transmission electron microscopy showed that the ECP1, ECP2 and ECP3 coliphages belong to family Myoviridae. Increasing host age lead to inhibition of coliphage infectivity. The ECP1, ECP2 and ECP3 coliphages exhibited different rates of adsorption and burst sizes but they possessed the same latent and rise periods (30 min). The host range of the three coliphages suggested that these coliphages may be useful as biocontrol agents. Addition of ECP1, ECP2 and ECP3 coliphages to their hosts decreased viable cell population of their host E. coli W1, W2 distinctively in vitro.
  Gamal Enan , Khalid A. Shaaban , Ahmed Askora and Muhammad Maher
  Three coliphages designated ECP1, ECP2 and ECP3 were identified in this study. Also, the effect of some physical factors on the infectivity of these coliphages was studied. The three coliphages were tested for their infectivity using the indicator bacterial strains E. coli W1 and E. coli W2 where coliphage ECP1 was highly specific to infect E. coli W1, on the other hand, coliphages ECP2 and ECP3 were highly specific to infect E. coli W2. The three coliphages were very sensitive to heat inactivation no survivors could be recorded after 10 min of exposure time up to 60°C. Also, the profile of the three coliphages inactivation after different time intervals exposed to UV-irradiation at different heights of 15 and 30 cm from the irradiation source was examined. Increased UV-irradiation doses decreased the plaque numbers of the three coliphages tested. The effect of different pH values on stability of the three coliphages was also tested. Maximum stability was observed at pH 6.0. The viabilities of the three coliphages were rapidly decreased towards alkalinity compared to pH 6.0. The ability of the three coliphages to form plaques at preservative temperature range 3-5, 25-28 and 35-37°C was examined. The suitable storage temperatures for the three coliphages were 3-5 and 25-28°C.
 
 
 
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