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Articles by Muhammad Bashir
Total Records ( 2 ) for Muhammad Bashir
  Muhammad Bashir , Zahoor Ahmad and Abdul Ghafoor
  In order to identify sources of resistance in cowpea against blackeye cowpea mosaic virus (BLCMV), two hundred cowpea germplasm accessions were evaluated under greenhouse conditions. Ten to 15 plants of each accession were artificially inoculated by sap prepared from virus-infected plants. Disease severity on each line was recorded at 15 days interval. The symptomless plants were tested by indirect enzyme-linked immunosorbent assay (ELISA) to separate the resistant plants from susceptible ones. Only two accessions (27168 and 27192) out of 134 of local origin were found resistant to BlCMV, whereas 23 (34.8%) were resistant out of 66 exotic lines. Twenty genotypes expressed segregation pattern towards disease reaction and the others were susceptible. In this study some new sources of resistance have identified which can be used to breed to breed virus-resistant cowpea cultivars.
  Takao Mitsueda , Muhammad Bashir , Zahoor Ahmad and Nabuo Murata
  The mechanism of "seed to seedling infection" of blight disease caused by Ascochyta rabiei (Pass.) Lab., was studied while growing naturally infected chickpea seeds and healthy seeds artificially inoculated with pathogen inoculum. The disease symptoms appeared on young seedlings within a week after emerging from soil. The seedling infection was due to contact of growing point to the pathogen inoculum from infected seeds during the process of shoot differentiation under the ground. Based on the observations of disease symptoms on the seedlings from contaminated seeds, it is concluded that contamination of plumule with pathogen inoculum during germination appears to be a major mechanism by which the pathogen transmission occurs on aerial parts of the plants.
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