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Articles by Muhammad Abubakar
Total Records ( 1 ) for Muhammad Abubakar
  Muhammad Akbar Shahid , Muhammad Siddique , Muhammad Abubakar , Muhammad Javed Arshed , Muhammad Asif and Arfan Ahmad
  In present study, status of Oxytetracycline (OTC) residues in chicken meat was determined in Rawalpindi/Islamabad area of Pakistan. Preliminary screening of samples for the presence of antibiotic residues was performed by a microbiological assay using Bacillus subtilis as test organism. OTC in positive samples was detected and quantified using High Performance Liquid Chromatography (HPLC) following extraction of the analytes with McIlvaine buffer and Solid Phase Extraction (SPE) system. A linear calibration curve was obtained with correlation coefficient of 0.9981 while average recoveries were greater than 91% with RSD values between 1.64 to 2.07%, while the Limit of Detection (LOD) was 0.01 μg mL-1. Out of 29 meat samples that were analyzed for OTC residues, 13 (44.8%) had detectable residue levels for OTC and 6 (20.7%) had higher residue levels than the recommended maximum residue level (0.2, 0.6 and 1.2 μg g-1) for muscles, liver and kidney, respectively. The method described in this study would be useful for routine monitoring of OTC residues in chicken meat.
 
 
 
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