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Articles by Muhammad Umar Dahot
Total Records ( 2 ) for Muhammad Umar Dahot
  Ikram-ul-Haq and Muhammad Umar Dahot
  An experiment was designed, to know what a physio-chemical situation developed in the micro-propagating banana variety Basrai on either organogenesis or shooting stage under specific hormonal conditions. In vitro plantlets were developed by culturing meristematic stem tips (explant) on organogenesis medium (8.0 μM BA; 10.0 μM IAA; 3.0 g L-1 phytagel) for 3 weeks, shoot induction medium (15.0 μM BA; 1.0 g L-1 phytagel) for 3 weeks and shoot multiplication medium (10.0 μM BA; 2.0 g L-1 phytagel) for 4 weeks. The micro-propagating plantlets and freshly excised explant from young banana plant from field were cultured on each of these 3 cultures separately. Variable morpho-biochemical contents were observed in each cultured tissue. On the organogenesis medium, reduction in Ca2+, K+, total protein and explant proliferation, fresh and dry weight, rate of micro-propagation but increase in Na+, Cl¯ and reducing sugar contents were measured, in comparison to both shooting cultures, where all of these were observed in a reversed form. So organogenesis resulted under stressed condition which is developed due to IAA and BA, later on relaxation leads into shoot induction, when only BA is present in the medium. Both steps i.e., organogenesis and shooting are directed due to the presence of specific hormones in the medium.
  Ikram- ul-Haq and Muhammad Umar Dahot
  The rate of banana micro-propagation depends on the balanced bio-chemical contents within the cultured explants, which are maintained by specific hormone/s in the medium. A normal mode of organogenesis and than shoot induction, are the hormonal derived steps for in vitro banana micro-propagation. To know about what a situation exist, under specific hormonal conditions, an experiment was designed. The meristematic stem tips were cultured for organogenesis on a number of media supplemented with NAA, BA and IAA separately and/ in a combination. After 2 weeks, the organogenised explants were sub-cultured on shoot induction medium, maximum plantlets (6.20±2.50) were observed on the medium supplemented with BA. Such efficient plant regeneration was occurred, when organogenesis was carried out on the medium supplemented with BA and IAA. Among these explants maximum Na+, Cl•, while decrease in Ca2+, K+, reducing sugar, total sugars and total protein contents were observed. All of these phenomena were reversed in the presence of BA only (shooting culture). These bio-chemical contents in the cultured tissue are specified due to the presence of s balanced form of specific hormone/s in the medium. A specific combination of hormones may be involved to direct organogenesis or shoot induction in the cultured explants.
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