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Articles by Muhammad Shahidul Haque
Total Records ( 3 ) for Muhammad Shahidul Haque
  Muhammad Shahidul Haque , Afifa Azad , Nihar Ranjan Saha and Muhammad Monirul Islam
  Background and Objective: Yardlong bean is a well-known vegetable because of its long, slender, succulent pods and its protein content. In the present study genetic variation and relationships among yardlong bean genotypes were investigated thorough RAPD marker technique. Materials and Methods: Thirty six genotypes of yardlong bean were planted in the field to characterize their genetic relatedness and identify variation at molecular level. Sixteen decamer RAPD oligonucleotide primers were screened and out of these, 10 primers were chosen for further evaluation. Results: Altogether 94 RAPD bands were scored of which 82 were polymorphic. The amplified product size ranged from 150-900 bp. The primer OPB-08 amplified maximum number of polymorphic bands. The average number of polymorphic bands/primer was 8.2. The frequency of polymorphism ranged from 0.0278-0.9722. Nei’s pair-wise genetic distance ranged from 0.055-0.83. A UPGMA dendrogram of genetic similarity was constructed based on 82 polymorphic bands obtained from 10 primers. The UPGMA dendrogram divided the studied individuals into four main clusters. BD-1528 and BD-1524 accession formed distinct cluster (I and II, respectively). Most of the genotypes were observed in cluster IV (29 genotypes) which was further sub-divided into two sub-clusters which were subdivided into sub-sub-clusters making the genotypes diverse. Accessions collected from Bangladesh Agricultural Research Institute, showed significant diversification as they were widely distributed in 4 clusters. Conclusion: The variability among the genotypes has the potential to be used in future plant breeding program as well as for commercial production.
  Muhammad Shahidul Haque , Tomikichi Wada and Kazumi Hattori
  Tissue culture is considered as an alternative tool for garlic breeding as traditional breeding is not possible due to its sterility. A study was carried out to develop a protocol of regeneration in Bangladesh local garlic. Shoot and root meristems of garlic cv. Bangladesh local were cultured on Murashige and Skoog (MS) medium containing various growth regulator combinations. Regeneration from shoot meristem was found with all medium composition but highest (95.55%) in growth regulator-free medium. Shoots were transferred onto media containing BA or high level of sucrose. Forty per cent (40.00%) of the root meristem regenerated shoots on MS medium supplemented with 1 µM NAA and 10 µM BA. The shoots were multiplied on MS medium containing 0.5 µM BA. Bulblets were obtained from the regenerated shoots of garlic in all the media. It is the first to report regeneration from any Bangladeshi garlic.
  Muhammad Shahidul Haque , Tomikichi Wada and Kazumi Hattori
  Root tips (2-3 mm) of garlic (Allium sativum L.) were cultured on MS medium supplemented with 1 FM NAA and 10 FM BA for shoot initiation. Initiated shoots were transferred to growth regulator free MS medium supplemented with 3, 6, 9 and 12% sucrose, 3% sucrose + 0.8% mannitol and 3% sucrose + 0.75 g l-1 KH2PO4 for proliferation and bulblet formation. The additives effectively proliferated the shoots. The highest number of shoots was produced by mannitol. Fresh weight (root and shoot) was highest in 6% sucrose and the longest shoots were found in the control (3% sucrose). Best root development in terms of root number and length was found in 6% sucrose. On the medium containing 9% sucrose, 90% of the shoots produced bulblets yielding the highest amount of bulblets/explant. However, maximum number of bullets/explant (smaller in size) was found on 6% and the heavier bulblets were found on 12% sucrose. The shoots on medium containing mannitol failed to produce bulblet and some of the shoots on 12% sucrose containing medium became abnormal and did not produce any bulblets.
 
 
 
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