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The result suggested that the fresh weights of the root tips decrease with increasing germination time at 25°C. The respiration rate decreases with increasing germination time and the pattern was very similarly in both quartiles. Waterlogging treatment showed large reductions on root growth and their fresh and dry weights. Waterlogging treatment of 12 h or more caused greater reductions in respiratory activities.
Fatty acid analysis in cotton seedlings following waterlogging showed changes in both their levels and relative composition. This treatment caused a marked increase in the amount of esterified fatty acids. Waterlogging also enhanced the synthesis of a set of low molecular weight compounds and suppressed the synthesis of all the “normal” fatty acids. A significant increase in free fatty acids was also observed.
The results of fatty acids analysis suggested that greater unsaturation occurred with increasing germination time. High temperature had a marked effect on fatty acid composition and caused significant changes especially in neutral lipid and glycollpid fatty acids. Small changes were also observed in phophollpid fatty acids. A large increase in free fatty acid was also found, since free fatty acids are known to be damaging to cells.
The experiment was carried out to study the effect of temperature on (Me-14C) choline uptake in cotton roots and its incorporation into phosphatidylcholine. The results suggest that (Me-14C) uptake and incorporation increased with increased germination time. Temperature above 35C had significant effect on (Me-14C) choline uptake and incorporation. Thus, as the temperature increased above 35C, the rate of synthesis of phosphatidylcholine decreased. At 50C, however, more than 80% of the phosphatidylcholine synthesis was inhibited.
