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Articles by Mostafa S.A. Khattab
Total Records ( 6 ) for Mostafa S.A. Khattab
  Ahmed M. Abdeltawab and Mostafa S.A. Khattab
  Palm kernel cake is a feed by-product that is used by the livestock industries. Chemical composition of palm kernel cake varies depending on the type of the fruits palm, source of sample and method of processing oil extraction (screw pressing or solvent extraction). This review was carried out to examine the effect of palm kernel cake on digestibility coefficients, daily weight gain, milk production and composition of animal. Varies treatments such as physical, chemical and biological were used to improve the nutrients digestibility and nutritive value of palm kernel cake. Several studies found that the animals performed satisfactorily well when fed on diets containing different levels of palm kernel cake. Palm kernel cake was used as feed for fattening and dairy cattle where as it has a source of protein, energy, vitamins and minerals. Digestibility coefficients for palm kernel cake were affected by adding it to animal's diets at high levels.
  Mostafa S.A. Khattab , Ahmed M. Abd El Tawab and Mohamed T. Fouad
  Background and Objective: Anaerobic bacteriadiversity in the rumen could be one of the keys of enhancing ruminant performance and productivity. The anaerobic population by its great diversity could be possible solution for some nutritional properties and enhance animal resistance to pathogenic microbes. Antimicrobial resistance for antibiotics used in animal production. The main effects of probiotics are the improved resistance to pathogenic bacteria colonization and enhanced host mucosa immunity; thus resulting in a reduced pathogen load, an improved health status of the animals and a reduced risk of food-borne pathogens in foods. This study aimed to isolate anaerobic probiotic from bovine rumen samples. Materials and Methods: Isolation were carried out using classical method by using selective medium then the isolates were identified using modern techniques by real time PCR and DNA sequencing techniques. Results: Results showed identified isolates were as Ruminococcus flavefaciens, Blautia obeum and Clostridium butyricum. These isolates showed a great cellulytic activity by producing cellulase enzyme with activity degree as 6543, 8555 and 5179 IU g–1 for Ruminococcus flavefaciens, Blautia obeum and Clostridium butyricum, respectively. Moreover, the activity of isolates for degrading tannins were 598, 1402 and 866 U g–1 for Ruminococcus flavefaciens, Blautia obeum and Clostridium butyricum, respectively. Clostridium butyricum showed superiority for inhibiting pathogenic bacteria (Yersinia enterocolitica, Bacillus cereus and Staphylococcus aureus) while, Blautia obeum recorded the highest antimicrobial activity against Salmonella typhimurium. Ruminococcus flavefaciens showed the higher activity against Listeria monocytogenes, Klebsiella pneumonia and Clostridium perfringens Conclusions: The results showed a potential properties of Ruminococcus flavefaciens, Blautia obeum and Clostridium butyricum to be used in as supplement to ruminant diet to enhance its performance and improve the health status through the antimicrobial activity of the isolated bacteria (Ruminococcus flavefaciens, Blautia obeum and Clostridium butyricum).
  Mostafa S.A. Khattab , H.H. Azzaz , Ahmed M. Abd El Tawab and Hussein A. Murad
  Background and Objective: Cellulases enzymes are widely be interested due to its capability to degrade lignocellulosic materials. The current study was concerning on investigating different factors of cellulase production from fungal sources by using agricultural wastes and studying its impacts on ruminal digestion and fermentation. Materials and Methods: The study tested the cellulase enzyme production ability of fungal strains against different lignocellulosic. Simultaneously the effect of different fungal strains to choose according to the superiority of cellulase production, then environmental factors were studied such as carbon source concentrations, inoculum size, incubation period, initial pH and nitrogen source. Finally, produced cellulase was evaluated using in vitro batch culture technique. Results: Penicillium chrysogenum recorded the highest value for cellulase activity. Pea pods showed best carbon source with 17.5% concentration for cellulase production. About 4% inoculum size, 2 days of incubation and pH5 were recorded the highest value for cellulase production. In vitro fermentation results showed improvement of DM digestibility compared with control. Conclusion: The current findings showed potential possibilities to utilize agricultural wastes as a substrate for producing cellulase enzyme from Penicillium chrysogenum fugal strain which could be an effective additive to improve ruminant diet digestion and utilization.
  Ahmed M. Abd El Tawab , H.A. Murad , Mostafa S.A. Khattab and H.H. Azzaz
  Background and Objectives: Enhancing poor quality roughages by biological treatments has interested for many researches in last years. So, in the current study was carried out to production of tannase enzyme by Aspergillus terreus and its impact on ruminal fermentation, degradability and gas production were evaluated through in vitro trials. Materials and Methods: Aspergillus terreus was grown as stand cultures in 100 mL conical flasks containing tannic acid powder medium. The maximum production of tannase by Aspergillus terreus was achieved at inoculum ratio of 4% (v/v), 72 h of incubation period, initial pH 5.0, urea as a nitrogen sources at a concentration of 0.33 g N L1 and pomegranate peel as a carbon source at a concentration of 10% (w/v). For animal feeding experiments in vitro dry matter, NDF, ADF, cellulose and hemicellulose disappearance and rumen fermentation were determined for the experimental diets. The concentrate:roughages ratio was 50:50 on DM basis, experimental diets plus different levels of tannase enzyme being 0, 5250, 10500, 15750, 21000 and 26250 IU kg1 DM were studied. Results: Tannase enzyme increased DM, NDF, ADF, cellulose and hemicellulose degradability of the treated diets compared with the control diet (0.0 IU kg1 DM), which gave the lowest values of diet degradability parameters. The maximum produced tannase efficiency percentage for dry matter degradability was obtained at 15750 IU kg1 DM. Production of tannase enzyme by Aspergillus terreus strain under solid state fermentation was superior over tannase production from other fungal strains under the submerged cultures. Conclusion: In conclusion tannase enzyme had positive improving of feed digestion in the current in vitro study.
  Mostafa S.A. Khattab , Ahmed M. Abd El Tawab , Fatma I. Hadhoud and Mahmoud M. Shaaban
  Background and Objectives: Improving ruminant environmental impacts and productivity get a great interest in last years, herbal plants were investigated as feed additive for decreasing gas production from rumen and enhancing nutrients digestibility. So, in the current study was carried out to investigate the effect of supplementing ruminant diets with different levels of thyme or celery on rumen fermentation, digestibility and gas production. Materials and Methods: Seven experimental treatments were done using rumen in vitro batch culture technique. Treatments were: 60% CFM, 40% clover hay (control), control diet+5 g thyme kg1 DM (T1), control diet+10 g thyme kg1 DM (T2), control diet+20 g thyme kg1 DM (T3), control diet+5 g1 celery kg1 DM (T4), control diet+10 g1 celery kg1 DM (T5), control diet+20 g celery kg1 DM (T6), control diet+0.4 g salinomycin kg1 DM (T7). Results: The obtained results showed no significant (p>0.05) change in ruminal pH, neutral detergent fiber (NDF) and acid detergent fiber (ADF) degradation. Thyme addition to diet (T1 and T2) significantly lowered (p<0.05) total gas production (TGP) compared with control (152, 152 vs. 157 mL, respectively). Dry matter and organic matter disappearance (DMd, OMd) appeared showed no significant difference (p<0.05) between control and thyme treatments (T1, T2 and T3) and celery treatments (T4, T5 and T6). Conclusion: It could be concluded the adding thyme or celery at low levels to ruminant diets could improve ruminal fermentation and reducing gas production without adverse effect on nutrients digestibility.
  S.A.H. Abo El-Nor and Mostafa S.A. Khattab
  There is an increase interesting in enrichment of milk with Conjugated Linoleic Acid (CLA) due to its anti-oxidative and anti-carcinogenic properties. The objective of this study was to investigate the effect of supplementing diets fed to lactating goats with sunflower, fish oil and its blend. Eight lactating Nubian goats were fed a base diet (T1), diet supplemented with 2% sunflower oil (on dry matter (DM) basis) (T2), diet supplemented with 2% fish oil (T3) and diet supplemented with 2% sunflower and fish oil (T4) for 84 day. Milk composition milk fat, protein (%) decreased in T2, T3 and T4 compared with control (T1) while there was no significant differences between treatments in milk lactose content. CLA content in milk fat was higher in response to fish oil or sunflower and fish oil blend compared with control (T1). The results indicated that supplementing diets fed to lactating goats with sunflower, fish oil increased CLA contents in the milk 2-4 times than control.
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