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| Articles
by
Mojtaba Tabatabaei Yazdi |
Total Records (
4 ) for
Mojtaba Tabatabaei Yazdi |
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Eshrat Gharaei-Fathabad
,
Mojtaba Tabatabaei Yazdi
,
Seyed-Naser Ostad
,
Shadman Shokravi
,
Zargham Sepehrizadeh
,
Mohammad Ali Faramarzi
and
Mohsen Amini
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A total of 100 supernatants and methanol extract samples obtained from three weeks’
incubation of 50 different isolate strains of cyanobacteria with paddy-fields origin were investigated for their cytotoxicity properties. Fifteen supernatants and 10 methanol extract samples exhibited cytotoxic activity against Balb-C cells while one methanol extract sample possessed a cytotoxic effect against five different cell lines including HeLa, Vero, Caco-2, HepG-2 and CHO more than vincristine, 5-fluorouracil and methotrexate when used at their IC50 concentrations. The later potent extract was achieved from the cyanobacterium strain GT-319. It was characterized as Nostoc piscinale Kützing ex Bornet and Flahault 1888 according to the morphological studies. Identification of the species was performed following molecular taxonomy by 16S rRNA sequencing. This is the first report on the cytotoxic activity of N. piscinale GT-319. |
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Mohammad Ali Faramarzi
,
Mojtaba Tabatabaei Yazdi
,
Mohsen Amini
and
Abbas Shafiee
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The whole cells of Acremonium strictum
transformed prednisolone at its side chain to produce two steroid compounds.
21,21-Dimethoxy-11β-hydroxypregn-1,4-dien-3,20-dione was the main
metabolite which its production has not been previously reported using
microbiological means. This metabolite together with a hydroxylated derivative,
11β-hydroxyandrost-1,4-dien-3,17-dione, were purified with preparative
TLC followed by their identification through 1H, 13C
NMR and other spectroscopic data. Best fermentation condition was found
to be 5 day incubation at 25 °C and pH value of 6 according to TLC
profiles. Optimum concentration of the substrate, which gave maximum bioconversion
efficiency, was 1 mg mL-1 in one batch. Biotransformation was
completely inhibited in a concentration above 5 mg mL-1. |
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Gholamreza Zarrini
,
Zargham Sepehrizadeh
,
Mojtaba Tabatabaei Yazdi
,
Qorbane Behzadiyan Nejad
and
Zuhair Muhammad Hassan
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Brucella abortus ribosomal L7/L12 protein is a 12 kDa protein encoded by rplL. This gene was amplified by PCR from B. abortus S19 and cloned in pET16b expression vector. The construct was transformed in Escherichia coli BL21 (DE3) PlysS. The expression was performed by addition 1 mM IPTG (isopropyl-beta-D-thiogalacto- pyranoside) in the medium with OD = 0.6 and incubation continued for 1, 2, 4 and 8 h at 37°C. The highest production (23 mg L-1) level was achieved after 4 h incubation of induction. The recombinant protein was purified by Ni-NTA column and characterized by SDS-PAGE. |
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Zahra Moradpour
,
Maryam Torshabi
,
Mohammad Ali Faramarzi
,
Mojtaba Tabatabaei Yazdi
,
Younes Ghasemi
,
Hoda Jahandar
,
Nadia Zolfaghary
and
Gholamreza Zarrini
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The ability of Nostoc ellipsosporum PTCC 1659, a cyanobacterium strain, for biotransformation of androst-4-en-3,17-dione (AD) was studied. Fermentation was performed in BG-11 medium supplemented with 0.05% AD at 25°C for seven days incubation. The single metabolite obtained was purified using chromatographically methods and characterized as testosterone on the basis of its spectroscopic features. Bioreaction characteristic observed was 17-carbonyl reduction. Time course study showed the accumulation of the product from the third day of the fermentation and reached to the maximum in the seventh day. Production of testosterone was not affected by aeration. Continuously illumination or 16 h light/8 h dark has no effect on the transformation yield. Optimum concentration of the substrate, which gave maximum bioconversion efficiency, was 0.5 mg mL-1 in one batch. Biotransformation was completely inhibited in a concentration above 2.0 mg mL-1. |
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