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Articles by Mohd. Yaqoob Wani
Total Records ( 4 ) for Mohd. Yaqoob Wani
  Hosakote Venkatappa Mohan , Ramswaroop Singh Rathore , Kuldeep Dhama , Thadiyam Puram Ramees , Anil Patya , Prashanth Suresh Bagalko , Mohd. Yaqoob Wani , Kiran Narayan Bhilegaonkar and Ashok Kumar
  Arcobacter is an important emerging food and water borne pathogen having worldwide public health concern. The present study reports the prevalence of Arcobacter spp. in humans, animals and foods of animal origin based on cultural isolation, antibiogram, Polymerase Chain Reaction (PCR) and multiplex PCR detection. A total of 400 samples were collected as human diarrheal stool (50), faecal swabs of poultry (50), pig (50), cattle (50) and foods of animal origin [Raw milk (60), chicken meat (60), beef (40) and pork (40)]. The overall prevalence rate of Arcobacter spp. was found to be 6.75% (27/400) by cultural isolation with highest prevalence in pig faeces (12%), followed by cattle faeces (10%), chicken meat (10%), poultry faeces (8%), beef (5%), pork (5%), human diarrheal stools (2%) and milk (1.67%). PCR screening revealed prevalence of Arcobacter spp. to be 7.75% (31/400) with highest in pig faeces (12%), followed by cattle faeces (12%), chicken meat (11.67%), poultry (10%), beef (7.5%), pork (5%), human stools (2.00%) and raw milk (1.67%). Multiplex PCR assay enabled detection of A. butzleri (21/27) and A. skirrowii (6/27). In vitro antibiotic sensitivity profile of 27 Arcobacter isolates revealed most of these to be sensitive to azithromycin, gentamycin, nalidixic acid, kanamycin, streptomycin, ciprofloxacin and tetracycline. Higher resistance was observed for cephalothin, novobiocin and vancomycin with notable intermediately resistance against erythromycin and chloramphenicol. The present study demonstrated high prevalence of Arcobacter spp. in pig, cattle and poultry faecal samples which may play important role in contamination of environment, water and human food chain, thus could be of public health concerns. The PCR was found to be more rapid, sensitive, specific and efficient than cultural methods for detection of Arcobacter spp.
  Kuldeep Dhama , Ruchi Tiwari , Sandip Chakraborty , Mani Saminathan , Amit Kumar , K. Karthik , Mohd. Yaqoob Wani , Amarpal , Shoor Vir Singh and Anu Rahal
  Owing to rising incidences of antimicrobial resistance against various chemotherapeutic and antimicrobial agents, the treatment of bacterial infections requires special consideration that may otherwise lead to grave prognosis. Simultaneously, evolution of many a Multiple Drug Resistant (MDR) bacterial strains have further aggravated the present situation. In this scenario, scrutinizing for some alternative yet effective antibacterial therapeutics like herbs, nutritional immunomodulators, bacteriophages, avian egge antibodies and others have become need of the day. Herbs have been a valuable source of medication in virtually all cultures and societies worldwide due to their important antimicrobial principles and phytoconstituents and wider therapeutic potentials. As various extracts of herbs and medicinal plants are being reported with antibacterial activities, much effort should be made in their identification, studying biologically active ingredients, efficacy and potency testing and scientific validation for their significant and practical multi-beneficial uses. The present review elaborates the potential role and applications of several herbs in treating bacterial infections and various types of bacterial diseases for safeguarding health of humans and their companion animals. It highlights the salient beneficial applications of traditional herbs and novel phytomedicines, from ancient periods to modern usages. Due emphasis has been given regarding scientific approaches to be followed and future perspectives with a vision to counter the emerging antimicrobial resistance. The review will certainly promote and popularize herbs as alternatives to conventional antimicrobials, particularly in the event of emerging MDR bacterial infections. Global usages of herbs as alternative and complementary medicines to various antimicrobials would lead not only to safeguard health issues and obtain optimum production from animals but will also ensure the public health issues including of food safety concerns viz., antibiotic residual effects in animal products (milk, meat) and zoonotic threats.
  Kuldeep Dhama , Sandip Chakraborty , Mahima , Mohd. Yaqoob Wani , Amit Kumar Verma , Rajib Deb , Ruchi Tiwari and Sanjay Kapoor
  Modern medicine has helped to a great extent to eradicate and cure several diseases of mankind and animals. But the existence of incurable diseases like cancer, Acquired Immunodeficiency Syndrome (AIDS), diabetes or rheumatoid arthritis, side effects of allopathic medicine, increasing trend of antibiotic resistance and chemicals and biopesticides causing dietary risk have made the situation more critical than ever before. Thus, it has become a matter of concern for the scientists and researchers to develop novel therapies. Bacteriophage therapy to treat pathogenic bacterial infections, virophage therapy for conservation of global system and avian egg yolk antibody therapy for designing prophylactic strategies against Gastrointestinal (GI) diseases are interesting approaches. Others include the use of cytokines as adjunctive immunomodulators, gene therapy focusing on diseases caused by single gene defects, RNAi technology to suppress specific gene of interest and apoptins for cancer treatment. Stem cell therapy against several diseases and ailments has also been discussed. The use of nanoparticles for better drug delivery, even though costly, has been given equal importance. Nevertheless, immunomodulation, be it through physiological, chemical or microbial products, or through essential micronutrients, probiotics, herbs or cow therapy prove to be cost-effective, causing minimum adverse reactions when compared to allopathy. Development in the field of molecular biology has created an enormous impact on vaccine development. The present review deals with all these novel and emerging therapies essential to safeguard the health of humans and companion animals.
  Ruchi Tiwari , Sandip Chakraborty , Kuldeep Dhama , Mohd. Yaqoob Wani , Amit Kumar and Sanjay Kapoor
  Darwin’s theory of natural selection and concept of survival of fittest of Wallace is a universal truth which derives the force of life among all live entities on this biosphere. Issues regarding food safety along with increased drug resistance and emerging zoonotic infections have proved that multidisciplinary efforts are in demand for human and animal welfare. This has led to development of various novel therapies the list of which remains incomplete without mentioning about phages. Homologous and non-homologous recombination along with point mutation and addition of new genes play role in their evolution. The rapid emergence of the antibiotic resistant strains of bacteria have created keen interest in finding necessary alternatives to check microbial infections and there comes the importance of phages. Phages kill the bacteria either by lysis or by releasing holins. Bacteriophages; the viruses that live on bacteria are nowadays considered as the best biocontrol agents. They are used as replacers of antibiotics; food industry promoter; guard of aquatic life as well as of plants; pre-slaughter treatment agents; Generally Recognized As Safe (GRAS) food additives; Typing agent of bacteria; active tool of super bug therapy; in post harvest crops and food and during post infection and also to combat intracellular pathogens viz. Mycobacteria and Mycoplasma. Cyanophages/phycophages are particularly useful in controlling blooms produced by various genera of algae and cyanobacteria. By performing centrifugation studies and based on electron microscopy certain virus like particles containing ds RNA have been confirmed as mycophages. They are well proven as threat to pathogenic fungi (both fungal hyphae and yeast). Those that infect yeasts are called zymophages. Virophages have exquisite specificity for their viral host, hence can extensively be used for genetic studies and can also act as evolutionary link. After the discovery of very first virophage till now, a total of 3 virophages have been discovered including the Sputnik virophages that are used to study genetic recombination. Virophages also find their application in antiviral therapy; as engineer of ecological system etc. In brief, present review deals with various dimensions of these beneficial viruses that are being used and can be successfully used in future for safeguarding biosphere including animal and human health.
 
 
 
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