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Articles by Mohd Zamri-Saad
Total Records ( 3 ) for Mohd Zamri-Saad
  Faez Firdaus Jesse Abdullah , Abdinasir Yusuf Osman , Lawan Adamu , Mohd Syamil Mohd Yusof , Abdul Rahman Omar , Abdul Aziz Saharee , Abd Wahid Haron , Rasedee Abdullah and Mohd Zamri-Saad
  Haemorrhagic Septicaemia (HS) is an acute, fatal, septicaemic disease of cattle and water buffaloes caused by Pasteurella multocida, serotype B:2 in tropical countries. The limitations associated with accurate predictions of mortality, survival levels and the detection of the presence of the organism from various organs of infected animals. Hence, this study used mouse model to evaluate the pattern of mortality and bacterial recovery from organs. Twenty-four mice were randomly divided into two groups. Infected group were inoculated orally with 109 colony forming unit of P. multocida type B, the group 2 were negative controls. The mice were observed for 5 days post-inoculation. At necropsy, visceral organs of dead animals were subjected for the confirmation using Polymerase Chain Reaction (PCR). The results showed that mortality rate was significantly different (p<0.05) between the infected and control groups. Within infected group, highly significant difference (p<0.05) was observed where 12.5% of the mortality rate was recorded within 24 h and 62.5% within 48 h post-infection. The survival rate, in infected group, was found to be around 25%. In diagnosis, P. multocida type B was detected from all organs of animals that did not survive. In contrast, P. multocida type B was neither recovered nor detected from the organs of mice which survived until the end of the experimental period (120 h). The results of this study indicated that manipulation of the organism in experimental animals provided clear information of the incidence of the disease in the field.
  Faez Firdaus Jesse Abdullah , Abdinasir Yusuf Osman , Lawan Adamu , Zunita Zakaria , Rasedee Abdullah , Mohd Zamri-Saad and Abdul Aziz Saharee
  Acute Phase Protein (APP) investigations of serum or plasma following natural or experimental infection frequently reveal substantial alterations in the APPs, several of which are of veterinary importance in the assessment of herd health. The present study of the experimental nature was conducted to evaluate the acute phase protein responses; haptoglobin, Serum Amyloid A (SAA) and serum albumin in relation to infection with Pasteurella multocida type B and its immunogens; lipopolysaccharide (LPS) and Outer Membrane Protein (OMP) in calves. Eight clinically healthy, non-pregnant and non-lactating Brangus cross calves weighing 150±50 kg were used in this study. The calves (n = 8) were divided into 4 groups of 2 calves in each group. The control group was inoculated with sterile Phosphate Buffered Saline (PBS) whereas group 2 were inoculated with wild-type P. multocida type B:2 and group 3 and 4 were inoculated with LPS and OMP respectively. Blood samples were collected via jugular vein-puncture at 3 h intervals for APPs analysis. APPs were quantified by commercially available ELISA methods. Moribund animals were euthanized while the surviving animals were killed after 48 h. The results revealed that there were statistically significant differences (p<0.05) between APPs concentrations throughout the experimental period in challenged groups compared to control groups. Over 7-fold increase was observed in Hp concentrations with mean maximum levels of 1.316±0.558 ng mL-1, 1.521±0.687 ng mL-1 after experimentally induced with OMP and LPS respectively. SAA increased less than 3-fold with mean levels of 2.187±0.880 ng mL-1, 2.421±0.432 ng mL-1, 2.657±0.099 ng mL-1 in almost all challenged groups; Pasteurella Multocida, OMP and LPS respectively. In contrast, for the negative APP, the albumin levels of groups LPS and OMP did not show significant difference (p>0.05) with mean levels of 32.677±1.556 and 36.185±2.239 U L-1, respectively. While P. multocida group (22.193±2.727 U L-1) showed statistically significant difference (p<0.05) than the negative control group (34.233±6.900 U L-1). In conclusion, the findings of this study indicated that APPs; SAA and haptoglobin are sensitive biomarkers to explore host response in relation to Haemorrhagic Septicaemia infections in clinical settings.
  Faez Firdaus Jesse Abdullah , Abdinasir Yusuf Osman , Lawan Adamu , Zunita Zakaria , Rasedee Abdullah , Mohd Zamri-Saad and Abdul Aziz Saharee
  Pasteurella multocida is an opportunistic pathogen with veterinary and human significance. The species diverse and complexity between the types exist with respect to antigenic variation, host predilection and pathogenesis. The aim of the present study was to determine hematological and serum concentration of biochemical values in calves having infected with Pasteurella multocida type B and its immunogens; Lipopolysaccharide (LPS) and Outer Membrane Protein (OMP). A total of eight clinically healthy calves were divided into four groups of 2 calves in each group. Group 1 were inoculated with sterile Phosphate Buffered Saline (PBS). Group 2 were inoculated with wild-type P. multocida type B: 2 while Group 3 and 4 were inoculated with LPS and OMP extracted from Pasteurella multocida type B, respectively. Blood samples were collected for haematological and biochemical analysis. Results indicated significant differences in the mean values of Prothrombin Time (PT), Partial Thromboplastin Time (PTT) and thrombocyte levels in infected groups 2-4 compared to the control group. There was significant decrease in the mean concentration of Alanine Transaminase (ALT) for group 2 (20.607±1.639 U L-1), group 3 (22.067±1.617 U L-1) and group 4 (40.327±8.351 U L-1). Conversely, there was no significant difference in Alkaline Phosphatase (ALP) for group 2 (149.857±17.327 U L-1) but a significant decrease for groups 3 (114.147±20.901 U L-1) and group 4 (128.971±17.068 U L-1). There was no significant differences in the serum levels of Gamma-glutamyltranspeptidase (GGT) and almost all tested electrolytes in infected groups. In conclusion, our results provide evidence in support of using some haematological and biochemical markers for early detection of individuals at risk for wild type of Pasteurlla multocida.
 
 
 
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