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Articles by Mohd Salleh Kamarudin
Total Records ( 3 ) for Mohd Salleh Kamarudin
  Saufinas Ismail , Mohd Salleh Kamarudin and Ehsan Ramezani-Fard
  Mahseer is one of the most expensive freshwater fish in Malaysia and has a good potential for freshwater aquaculture industry. This study was conducted to evaluate the suitability of Poultry Offal Meal (POM) as Fish Meal (FM) replacement in the diet of Malaysian mahseer (Tor tambroides). The POM:FM ratios tested were 0:100, 25:75, 50:50, 75:25 and 100:0. One hundred and fifty mahseer fingerlings (3.0±0.05 g) were equally and randomly stocked into 15 65 L aquaria. The diets were randomly assigned to the aquaria in triplicates groups. The feeding trial was conducted for 96 days. No significant differences (p>0.05) were observed among the survival rate of fish while it was above 90% in all the treatments. Weight gain and specific growth rate significantly increased when 50-100% of dietary fishmeal was substituted with POM. Whole body composition of Malaysian mahseer was not significantly influenced by the experimental diets. This study showed that fishmeal in the diet of mahseer can be replaced by POM up to 100% ratio without any adverse effect on survival, growth performance and body composition.
  Hadi Zokaei Far , Che Roos B. Saad , Hassan Mohd Daud , Mohd Salleh Kamarudin and Ehsan Ramezani-Fard
  The emergence of antibiotic-resistant bacterial pathogen has led aquaculture attentions to the use of probiotics as an alternative to antibiotics. This study was conducted to isolate bacterial micro flora from digestive tract of healthy juvenile white shrimp, Litopenaeus vannamei based on antagonistic activity against shrimp pathogen, Vibrio parahaemolyticus. In this study, potential probiotic strains were isolated using replica plating method to screen the bacteria with antagonism properties. Six isolates were identified as Pseudomonas sp. using conventional biochemical tests and Biolog GN microplates. In addition three Vibrio species including V. parahaemolyticus, Vibrio alginolyticus and Vibrio cholerae were isolated from moribund shrimp and used as pathogen in this study. The pathogenicity of three Vibrio species was tested on shrimp L. vannamei by injecting 0.1 mL of 107 CFU mL-1 of each pathogen into the third abdominal segment. Among three Vibrio species only V. parahaemolyticus found to be highly virulent to shrimp with 43% cumulated mortality after 10 days. Subsequently, all Pseudomonas sp. isolates were tested for antibacterial activity against three Vibrio species using cross streak assay. Strong antibacterial activity was recorded for Pseudomonas sp. isolates number 5, 7, 15 and 30 against three pathogens. In addition a reasonable antibacterial activity was observed for isolates number 9 and 12. On the basis of great antibacterial activity of Pseudomonas sp. isolates these species may be considered for future challenge experiments in shrimp as a very promising alternative to the use of antibiotics.
  Nur Syuhada Mat Noor , Aziz Arshad , S.M. Nurul Amin and Mohd Salleh Kamarudin
  Background and Objective: Copepods are being used widely as live feed in aquaculture industry particularly for larval rearing of fish and crustacean. Several marine copepods have been cultured successfully before but the numbers of species documented is still limited. Cyclopoid copepod, Oithona simplex is readily available in the Malaysian coastal waters and their high abundance is observed in the coastal waters of Port Dickson, Malaysia. It is targeted as a potential live food for larval rearing of marine fish species. The present study was conducted to determine the effect of environmental parameters viz. salinity, temperature, light intensity and photoperiod on the reproductive cycle of cyclopoid copepod, Oithona simplex under laboratory condition. Materials and Methods: Four different salinity levels (20, 25, 30 and 35 ppt), three different temperatures set up (25, 30 and 35°C), three light intensities expose (5, 20, 35 μmol m2 sec1) and three different photoperiod phases (12 h light (L):12 h dark (D), 1 h (L):23 h (D), 24 h (L):0 h (D) were adopted during this experiment. Results were subjected to one-way ANOVA. Results: The results showed that the optimum salinity was recorded at 30 ppt for the highest production of off springs. Similar highest production was found at 35°C temperature setup. The optimum light intensity expose was under 5 μmol m2 sec1 and this was reflected by the increase in reproductive activity and decreased in development period. Subsequently, 12 h light:12 h dark treatment resulted in the highest reproduction process. Conclusion: It could be recommended that optimal environmental condition which comprised of 30 ppt salinity, 35°C temperature, 5 μmol m2 sec1 light intensity and alternate 12 h light and dark photoperiod is required for culturing of O. simplex in captivity. This is the first attempt to breed and mass production in controlled environment. The information gathered from the experiment could be helpful to produce a mass culture of O. simplex indoor for the purpose of live food supply in the hatchery.
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