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Articles by Mohd Hair-Bejo
Total Records ( 6 ) for Mohd Hair-Bejo
  Mozhdeh Emadi , Fatemeh Jahanshiri , Kamran Kaveh , Mohd Hair-Bejo , Aini Ideris and Razak Alimon
  Infectious bursal disease is still a challenging issue by posing a serious threat to the commercial poultry industry especially due to the emergence of highly Infectious Bursal Disease Virus (IBDV). In the present study, we evaluated the immunomodulatory effects of Tryptophan (Trp) on innate, humoral and cellular immune responses in chickens challenged by oral administration of intermediate plus strain of IBD virus at 28 days of age. A corn-soybean meal based diet containing different levels of Trp (0, 0.10 and 0.20) for the starter, (0, 0.07 and 0.15) for the grower and (0, 0.05 and 0.13) for the finisher has been utilized. In a completely randomized design with three treatments of five replicates each and 10 chickens per replicate, 150 Cobb 500 male broiler chickens from 0-49 days of age were subjected to Trp diet. To measure the innate, cellular and humoral immunity indicators (interferon-α, interferon-γ, immunoglobulin G, respectively) at 27, 35, 42 and 49 days of age, serum samples from each replicate of treatments were collected and subjected to ELISA. The result showed that Trp supplementation in the chickens basal diets significantly increased the serum levels of interferon-α at 35, 42 and 49 days of age (p<0.05), interferon-γ at 27, 35 and 49 days of age (p<0.05) and immunoglobulin G at 27, 35, 42 and 49 days of age (p<0.05). These results strongly suggest that tryptophan plays a vital role in modulation of protective immune response against IBDV.
  Mozhdeh Emadi , Kamran Kaveh , Fatemeh Jahanshiri , Mohd Hair-Bejo , Aini Ideris and Abdul Razak Alimon
  In order to study the effect of Tryptophan (Trp) on growth performance and blood parameters of broiler chickens, an in vivo experiment was conducted. A corn-soybean meal based diet containing different levels of Trp (0, 0.10 and 0.20) for the starter, (0, 0.07 and 0.15) for the grower and (0, 0.05 and 0.13) for the finisher was used. In a completely randomized design with three treatments of five replicates each and 10 chickens per replicate, 150 Cobb500 male broiler chickens from 0-49 days of age were subjected to Trp diet. Growth performance (body weight gain, feed intake and feed, gain ratio) and blood serum (albumin, total protein, glucose, cholesterol, triglyceride, urea, uric acid, aspartate amino-transferase, alanine amino-transferase, alkalin phosphatase, lactic dehydrogenase and creatine kinase) parameters were measured at 27 and 49 days of age. As the result showed the increase of dietary Trp elevated (p<0.05) body weight gain, feed intake, albumin, total protein, glucose, urea and uric acid and decreased (p>0.05) feed gain ratio, aspartate amino-transferase, lactic dehydrogenase, triglyceride and cholesterol. Therefore, we conclude that dietary Trp might have positive effects on health status of the broiler chickens.
  Saeed Sharif , Siti Suri Arshad , Mohd Hair-Bejo , Abdul-Rahman Omar , Nazariah Allaudin Zeenathul , Nor-Alimah Rahman and Amer Alazawy
  Feline Coronavirus (FCoV) comprises virulent and avirulent biotypes. While both biotypes can enter the bloodstream of a cat, only the virulent biotypes would replicate in monocytes and macrophages and develop a fatal disease known as Feline Infectious Peritonitis (FIP). In the present study, FCoV viraemia was evaluated in 50 cats consisting of 40 overtly healthy and 10 ill cats suspected of FIP. The blood samples were screened for FCoV genomic RNA by a RT-PCR assay and then followed by a duplex RT-PCR for detection of replicating viral mRNA. In the healthy cats, the virus and its replicating mRNA were detected in 67.5 and 15%, respectively. The later finding suggested that the virus was replicating in a few cats with no clinical sign shown and indicated that FCoV viraemia do not necessarily lead to FIP. Probably the avirulent virus does multiply at low level in the blood or cat can harbor the virulent virus in an early stage of FIP without clinical signs yet. In FIP-suspected cases, all of the ill cats were positive for both FCoV and the replicating viral mRNA suggesting that FCoV could have replicated in blood and produced high amount of the virus and its components which were detectable by the both assays. The duplex RT-PCR assay which has been used to detect the replicating viral mRNA in blood was more specific than the general screening RT-PCR test for the diagnosis of FIP. The RT-PCR results however, should be interpreted in conjunction with other clinical symptoms.
  Zahraa Faisal , Aini Ideris , Mohd Hair-Bejo , Abdul Rahman Omar and Tan ChingGiap
  The detection of Mycoplasma gallisepticum (MG) infection in chickens in selected commercial farms (breeder, broiler and layer), Progeny-pipped Embryos (PE) and day old chicks Poor Quality Chicks (PQC) and Normal Chicks (NC) obtained from Peninsular, Malaysia was determined. These farms have the history of vaccination with mild live and killed MG vaccine or antimicrobial prophylactic or treatment. To conduct this study, a total of 3056 samples were collected and conventional PCR test was performed using specific gene target sequence, encoding the surface protein for detection of MG directly from the clinical samples without prior isolation of the target MG. The primer was designed to bind to the adherence protein a (GAPA) and amplify a 505 bp DNA fragment and there are no secondary copies of this gene. Results revealed that five hundred seventy one positive samples of MG out of 3056 samples were obtained and the overall prevalence was 18.68%. This study shows high detection of MG infection through vertical and horizontal transmission from many geographically distinct areas of the country although, these farms had vaccination and treatment history. So, these results demonstrated that MG eradication program is not successful, despite the use of live and killed MG vaccines, an extensive medication program and strict biosecurity. It is recommended that PCR assay is used to monitor broiler breeder flocks for MG challenge during the laying cycle using progenies and confirm that point of lay broiler-breeder pullets are free of field MG strain. In addition to the importance of flock health monitoring in progenies, especially those pipped embryos and poor quality chicks and especially breeders in order to provide MG-free progeny.
  Zahraa Faisal , Aini Ideris , Abdul Rahman Omar , Mohd Hair-Bejo and Tan Ching Giap
  A total of 571 Mycoplasma gallisepticum (MG) field isolates originated from progenies and commercial poultry farms in Malaysia and 7 reference and vaccine strains were characterized by amplification of selected gene target specific sequences to MG pMGA and pvpA genes using conventional PCR of sequence specific primers. A total of 281 MG positive field isolates out of 571 MG samples were detected with the primer targeted pMGA gene and a total of 188 MG positive field isolates out of 571 MG samples were detected with the primer targeted pvpA gene. Similar and identical banding pattern among MG isolates obtained from progenies samples however, there was a variable on the banding pattern among MG isolates obtained from commercial chickens using the agarose gel electrophoresis. The sequencing analysis results of MG based on selected genes targeted specific sequences were obtained. The genetic diversity of the pMGA and pvpA genes of MG field isolates detected in progenies and commercial chickens were investigated. The gene size variation patterns of the pMGA and pvpA genes among MG field isolates shared identical variations with the pathogenic reference and vaccine strains that is an insertion bp fragments by using the pMGA gene primer set and a deletion bp fragments by using the pvpA gene primer set. However, the gene size variation patterns are quite different from the variation pattern of the less pathogenic vaccine strain that can’t be transmitted vertically. The polymorphism pattern of the primer for pMGA gene might be considered as a pathogenic vertical marker and the polymorphisms patterns of the two primers sets for both pMGA and pvpA genes might be useful for determining the two genetic potential pathogenic marker for MG infection that can differentiate between the highly and the less pathogenic MG isolates.
  Mukminah Sakinah Wahab , Mohd Hair-Bejo , Abdul Rahman Omar and Aini Ideris
  Infectious Bursal Disease (IBD) is a highly contagious viral disease of chickens. Application of liposomes in IBD vaccine may enhance vaccine delivery and induce high immune response. It was objective of the study to determine the effect of cationic liposomes in induction of IBD antibody. The 85 days old broiler chicks were divided into 4 groups namely the control, IBD, Covac and Sevac groups. The chicks in the control group were not vaccinated. The IBD group was vaccinated with commercial IBD vaccine, MyHatch UPM93 (103 EID50/0.1 mL) whilst the Covac group was vaccinated with combination 1:2 ratio of MyHatch UPM93 and cationic liposomes. The Sevac group was vaccinated with combination 1:2 ratio of the IBDV seed virus (103 EID50/ 0.1 mL) and cationic liposomes. The commercial and IBD seed virus were used in this study to determine the most effective and practical approach in application of liposomes during vaccine preparation. The chicks were vaccinated via. subcutaneous route (0.1 mL per chick) at day old in hatchery or hatchery vaccination. All groups of chicks were sacrificed at days 7, 14, 21 and 28 post vaccination (pv) for samples collection, except 5 chicks were also sacrificed prior to vaccination at day-old age. The study showed that all chicks did not exhibit any abnormal clinical signs and gross lesions throughout the trial, except reduced in bursal weight were recorded at day 28 pv in the IBD, Covac and Sevac groups. Histologically, lesion scoring of the bursa of Fabricius was slightly increased at days 21 and 28 pv in the Covac and Sevac groups and day 28 pv in the IBD group. The IBD antibody titre for the Covac and Sevac groups were also significantly (p<0.05) increased at days 21 and 28 pv and day 28 pv in the IBD group. It was concluded that the application of cationic liposomes can enhance the delivery of IBD vaccine to the bursa of Fabricius and induce high level of IBD antibody with mild bursal lesion.
 
 
 
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