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Articles by Mohammed Arifullah
Total Records ( 2 ) for Mohammed Arifullah
  Fawzia Jassim Shalsh , Noor Azlina Ibrahim , Mohammed Arifullah and Anis Shobirin Meor Hussin
  Bioethanol production using lignocellulosic biomass has gained increased attention because of the abundant supply of this biomass. Saccharomyces cerevisiae is a commonly used microorganism for ethanol production. Nevertheless, S. cerevisiae can not ferment xylose, the second most abundant sugar in plant tissues. In this study, protoplast fusion with the xylose-fermenting yeast Pichia stipitis was performed to improve bioethanol production from biomass. The protoplast formation of S. cerevisiae and P. stipitis (ATCC 58785) cells was achieved using zymolase 20T. The effects of zymolase concentration, enzymatic treatment time and osmotic stabilizers were further investigated. The optimal parameter for the protoplast release of S. cerevisiae and P. stipitis included 500 μg μL–1 zymolase for 60 min and 750 μg μL–1 zymolase for 120 min, respectively. The maximum protoplast formation ratios were 98.48 and 84.42% for S. cerevisiae and P. stipitis, respectively, with 1 mol L–1 sorbitol as the osmotic stabilizer. About 4×106 mL–1 protoplasts from S. cerevisiae and P. stipitis were isolated. Protoplast fusion frequency was determined using polyethylene glycol (PEG) as fusogen. The optimized fusion conditions of S. cerevisiae protoplasts with P. stipitis required 35% (w/v) PEG 6000, 10 mM CaCl2 level and 30 min of fusion time. The protoplast fusion rate was 52.21% under the optimized fusion condition.
  Ahmed Mahmood Ibrahim , Fatimah Binti Kayat , Dwi Susanto , Pedram Kashiani and Mohammed Arifullah
  Regeneration into haploid plantlets had been obtained in spring onion using flower and ovary culture. Flowers and ovaries were cultured into media using two protocols (A and B) and the ability to produce callus or somatic embryogenesis were invistigated. Flowers around 3.0-5.0 mm long were collected, whole flower or ovary which were excised from flowers using dissecting microscope were cultured into BDS medium supplemented 2.0 mg L–1 2, 4-D and 2.0 mg L–1 BAP fortified with 100 g L–1 sucrose, 200 mg L–1 proline, 500 mg L–1 myo-inositol (protocol A) or into BDS media supplemented 2.0 mg L–1 2,4-0 and 2.0 mg L–1 BAP for 14 days, then sub-cultured by regeneration medium (BDS) supplemented with 1.0 mg L–1 NAA and 2.0 mg L–1 2iP (protocol B). Embryos were emerged from ovary wall after around 4-5 months, high frequency of embryogenesis induction was produced from ovaries that were using protocol A. While the less percentage observed from flower culture using protocol B.
 
 
 
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