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Articles by Mohammed S. Alhussaini
Total Records ( 4 ) for Mohammed S. Alhussaini
  Mohammed S. Alhussaini , A.M. Saadabi , Mohammed I. Alghonaim and Khalid Elfakki Ibrahim
  The present work was aimed to evaluate oleo-gum resins aqueous, methanol and chloroform extracts of Commiphora myrrha for antimicrobial activity against four types of bacteria and twelve species and strains of fungi. All of the oleo-gum resin extracts irrespective of their types inhibited the growth of all microbes to varying degrees. Aqueous extract showed the least antibacterial and antifungal activity against all of the pathogens used especially with regard to gram positive bacteria Staphylococcus aureus (11 mm) and Escherichia coli (11 mm) in concentration of 100 mg mL–1 as compared to methanol or chloroform. Less or no activity was observed against Trichophyton concentricum (7.01 mm) and 4.01 mm inhibition zone for Candida rugosa in the same concentration used. The oleo-gum resins methanol extracts in different concentrations were significantly inhibitory to the growth of the different tested fungal dermatophytes. Reduction in mycelial weight of fungi was directly correlated with concentration of extract. The concentration of 200 mg mL–1 of myrrha was the most inhibitory against Epidermophyton floccosum with 2 g mycelial fresh weight while the less inhibition in the growth was obtained in Candida albicans and it was 6.61 g in the same extract concentration. The other used fungal or bacterial groups showed varying degrees of activity pending on the type of extract used. The Minimum Inhibitory Concentration (MIC) values of methanol and aqueous extracts of oleo-gum resins of myrrh showed that the highest values were obtained in methanol extract for Trichophyton concentricum and lowest MIC values for the same extract for the bacterium Staphylococcus aureus. In the aqueous extract of myrrha the highest and lowest MIC values were found for Bacillus subtilis and Pseudomonas aeruginosa. Phytochemical analyses showed the presence of Sesquiterpenes (Isoprenoids i.e., Terpenoids) and Furanosesquiterpenes as major constituents of the oleo-gum resins of the plant. These results confirm the antibacterial and antifungal activity of gum resins and support the traditional use of the myrrh in therapy of bacterial infections.
  Mohammed S. Alhussaini , Mohamed Adbo Moslem , Mohammed I. Alghonaim , Abdullah A. Al-Ghanayem , Abdulrahman A.I. AL-Yahya , Hamido M. Hefny and Adbul Moneam Saadabi
  Background: This investigation compared genetic similarities and diversities within and among Cladosporium species populations using the two PCR-based markers; Internal Transcribed Spacer (ITS)-PCR and microsatellite-PCR. Methodology: Nuclear ribosomal DNA internal transcribed spacers have been used to analyze intraspecific and interspecific relationships in various fungi. In the present study, the internal transcribed spacer (ITS)-PCR and microsatellite-PCR were used to identify the genetic diversities in Cladosporium species. Results: The Internal Transcribed Spacer (ITS) was amplified using polymerase chain reaction combining primers ITS4 and ITS5. The PCR products were digested with three restriction enzymes and separated by agarose gel electrophoresis. Restriction patterns generated by CfoI and Msp I and RsaI were unique for most species assayed. The ITS-PCR fingerprinting methods led to a clear differentiation of the isolates at the species level. Fingerprinting profiles generated readily discriminated between each of the 6 species. Cluster analysis further supported this observation and clusters corresponding to each species were readily identified in the dendrograms. Seven microsatellite primers out of eight primers were unable to generate visible DNA fingerprints. Conclusion: Amplification experiments demonstrated that microsatellite primer, T3B and (GTG)5 are technically simple tools for assaying genetic variability in Cladosporium spp.
  Mohammed S. Alhussaini
  Background and Objective: Methicillin-resistant Staphylococcus aureus (MRSA) have been causing increasing problems in hospitals and nursing homes worldwide. Limited number of studies in Saudi Arabia has attempted to investigate infection and risk factors associated with nosocomial acquired MRSA. The present study was undertaken to determine the occurrence, prevalence, antibiotic susceptibility pattern and genetic characteristics of MRSA among admitted cases at Shaqra General Hospital (Saudi Arabia). Methodology: This study was conducted from October, 2014 to March, 2015. Nasal swabs were taken from 220 patients (105 males and 115 females) admitted at Shaqra General Hospital. The isolates were identified as S. aureus based on morphology, Gram stain, catalase test, coagulase test and mannitol salt agar fermentation. Antibiotic susceptibility testing of MRSA was performed with standard disk diffusion method. All methicillin-resistant isolates were examined for the existence of the mecA gene by PCR technique. Results: Of the 220 patients, 90 (40.91%) were found to be nasal carriers of S. aureus. Among these 90 S. aureus isolates, 48 (21.82%) were MRSA. A statistically significant difference was only found for antibiotics usage between those with and without MRSA colonization. Antibiotic susceptibility pattern of isolated MRSA showed high susceptibility to vancomycin, linezolid, rifampicin, teicoplanin, complete resistance to penicillin, ampicillin, oxacillin and cefoxitin and intermediate resistance to amikacin, ciprofloxacin, teicoplanin, tetracycline and vancomycin. Conclusion: A high prevalence of multidrug-resistant MRSA nasal carriage was found. The identification of MRSA carriers is a step towards establishing a control policy for MRSA and helps to identify measures needed to reduce colonization pressure.
  Mohammed S. Alhussaini , A.M. Saadabi , Kamal Hashim and Abdullah A. Al- Ghanayem
  The aim of the present study was to apply natural drops of truffle (Terfezia claveryi) to patients diagnosed with trachoma for curing the disease and inhibiting the chlamydial bacteria and explore the potential for the development of new treatment modalities compared to currently used antibiotics. Results showed that the truffle juice was very effective against trachoma in the third phase of the disease. There was clear reduction of symptoms in patients with cortisone compounds in the form of drops plus truffle juice drops. There were also complete healing of the conjunctiva and healing of the keratitis. Partial curing was observed when cortisone plus cream of chloramphenicol was added included healing of trachoma without any change of fibrosis in the conjunctiva. In the treatment of trachoma with cortisone drops and cream plus truffle juice, complete of healing was observed but continued swelling in the capillaries with no fibrosis occurred. It was clearly noticed that in all of the treatments, there was a noticeable reduction in fibrosis of the affected site in the eye. It was also concluded that truffle juice seems to prevent fibrosis of trachoma by a large extent. On the other hand, the use of chemical antibiotic or cortisone-based compounds produced several side effects. Though these chemicals reduced the toxins caused by trachoma thereby reducing the enlargement of the cells. We can therefore clearly recommend adding truffle juice to the traditional treatment for trachoma at all stages.
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