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Articles by Mohammed M. Salih
Total Records ( 3 ) for Mohammed M. Salih
  Salah , M. M. Elamin , H. Salah Idris , Mohamed A. Abdalla , Badr E. Hago , Mohammed M. Salih and Rihab Omer
  A complementary DNA (cDNA) probe, derived from genome segment 6 (NS1) of epizootic hemorrhagic disease virus (EHDV) serotype 1 (EHDV-1), was synthesized by polymerase chain reaction (PCR) and evaluated for detection of Sudanese EHDV serogroup. A pair of primers (P1 and P2) was designed from NS1 genome segment of EHDV-1 and used for synthesis of a 387-bp cDNA probe. The cDNA probe hybridized with dsRNA from Sudanese EHDV serotypes including EHDV serotypes 4 and an untyped isolate designated (EHDV-318). However, dsRNA from blue tongue virus serotype 1, 2, 4 and 16 failed to hybridize with the cDNA probe. The result of this study indicated that, the developed cDNA probe could be used for rapid detection and differentiation of EHDV serogroup in cell culture.
  Salah M. M. Elamin , Salah H. Idris , Mohammed M. salih and Rihab A. Omer
  A reverse transcriptase polymerase chain reaction (RT-PCR) protocol was evaluated for detection of bluetongue virus ribonucleic acid in cell culture. BTV serotypes 1, 2, 4, 5, 10, 11, 13, 16 and 17 were studied. RNAs from these BTV serogroup, propagated in cell cultures, were detected by the described RT-PCR-based assay. The specific 519 bp PCR products were visualized on ethidium bromide-stained agarose gel using a pair of primers derived from segment 6 of BTV 11. Amplification product was not detected when the RT-PCR-based assay was applied to RNA from epizootic hemorrhagic disease virus (EHDV) or palyam virus serogroups; or total nucleic acid extracts from uninfected Vero cells. The results of this study indicated that the described RT-PCR assay could be applied for detection of BTV serogroup. In addition, the described BTV RT-PCR assay could be used as a supportive diagnostic assay to the current conventional virus isolation procedures used for detection of BTV in cell cultures.
  Salah M.M. Elamin , Tawfig E. Mohammed , Mohammed M. Salih , Mohammed A. Abdalla , Mohamed E.H. Mohamed , Rihab A. Omer , Abdelrahim Karrar and Imadeldin E. Aradaib
  The double stranded RNA (dsRNA) genome segments of the Sudanese isolates of epizootic hemorrhagic disease of deer virus (EHDV) were analysed by agarose gel and sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (SDS-PAGE). EHDV serotype 1 and 2 are enzootic in the United States whereas EHDV-4 and an untyped isolate designated EHDV-318 are enzootic in the Sudan. The dsRNA genome segments profiles of the Sudanese EHDV serotypes 4 and EHDV-318 were compared with those of North American EHDV serotypes 1 and 2. Both systems (agarose gel and SDS-PAGE) showed 10 segments for each virus isolate, which is characteristic pattern of EHDV serogroup. The agarose gel showed identical genome profiles for all Sudanese and North American serotypes of EHDV serogroup. However, SDS-PAGE system was able to detect genetic variation between Sudanese and North American EHDV serogroups and among the Sudanese serotypes of EHDV. The results of this study suggested that, the agarose gel electrophoresis could be used as a supportive or complementary method to facilitate tentative diagnosis of EHDV infection in susceptible animal populations. In addition, the SDS-PAGE could also be used to detect genetic diversity among topotypes of EHDV serogroup from the African or North American continents.
 
 
 
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