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Articles by Mohammed El Damir
Total Records ( 3 ) for Mohammed El Damir
  Mohammed El Damir
  The objective of this study was to evaluate conidial production of two isolates of Beauveria bassiana (Balsamo) (5672 and CA 44) and one of Metarhizium anisopliae (Metschnikoff) (500B) using corn, wheat and millet and three water volumes (substrate: water) (1:0.5), (1:1) and (1:1.5). We found that M. anisopliae tended to produce more conidia overall than the B. bassiana isolates. For the B. bassiana isolates, conidial production on wheat was higher for the 1:1 and 1:1.5 ratio than the 1:0.5. No significant differences were found in conidial production for 5672 on corn and millet at the volume regimes tested; whereas, for CA-44 on corn and millet, conidial production tended to decrease with increasing water volume. In contrast, conidial production of M. anisopliae increased up to 8.6 and 2.9 times between the lowest and highest water volume ratio on corn and millet, respectively. Wheat tended to produce more conidia depending on the water volume used, although only slight increases were observed for 5672. We found that conidial production was positively influenced by increased water ratio, particularly for M. anisopliae (500B). Depending on the combination of isolate, growing media and water volume, differences up to 240 times were observed. Such difference can have dramatic effect on the economical production of entomopathogenic fungi for field application. Present results provide useful information for developing a simple mass production tactic for targeting Sunn Pest in their overwintering sites.
  Mohammed El Damir
  The objectives of this study were to test the use of a forewing of the house fly (Musca domestica L.), ¼ strength sabauroud dextrose agar (SDAY/4) and dry agar-agar as substrates to assess the effect of relative humidity (RH) (91, 95, 98 and 100 ± 1%) and temperature (15, 20 and 30 ± 1 °C) on spore germination of Beauveria bassiana 5672 and Metarhizium anisopliae 1080 isolates at 24, 48 and 72 h post exposure. In all treatments tested, similar influences of temperature and %RH were observed when conidial germination of 5672 and 1080 was tested on SDAY/4 and fly forewing, whereas little or no germination occurred with agar-agar as a substrate. Conidial germination occurred at temperatures of 15-30 °C and >=95% RH and temperatures of 20-30 °C and >95% RH for B. bassiana 5672 and M. anisoplia 1080, respectively. At 100% RH, 30 °C and 72 h exposure period, the highest germination rates of 1080 were 99 and 96% on SDAY/4 and fly forewing, respectively. We concluded that the SDAY/4 medium triggered the conidial germination of these fungi similar to the house fly forewing cuticle. This suggests that SDAY/4 medium is useful as a substitute substrate of cuticle when examining the influence of temperature and %RH on conidial germination. The SDAY/4 medium is also nutritive and supplies a substrate for growth and sporulation. This technique provides a tool for predicting the epidemiology of these fungi in the field for an IPM program of Sunn Pest.
  Mohammed El Damir
  The objectives of this study were to evaluate spore germination, virulence against a surrogate host [the western flower thrips, Frankliniella occidentalis (Pergande)] and spore production capabilities at different temperatures and RH combinations of 6 single spore isolates newly obtained from multispore isolates of Beauveria bassiana (Balsamo) Vuillemin (5672) and Metarhizium anisopliae (Metschnikoff) (1080). We found that temperature and RH influenced conidial germination, spore production and virulence of 5672 and 1080 and their single spore isolates tested. At =98% RH, 20 and 30 °C, single spore isolates from 5672 had higher %germination than their multispore isolate, while at 100% RH and all temperature tested, the single spore isolates (1080-1 and 1080-2) had higher germination than their multispore isolate 1080. At 95% RH, the conidial germination was poor and only occurred at 20 °C for two single spore isolates (5672-4 and 5672-5) as well as for the multispore isolates 5672 and 1080. Metarhizium anisopliae isolates were more virulent against western flower thrips than the B. bassiana isolates tested. At 100% RH, mortality of western flower thrips was more obvious than at other %RH levels tested. Single spore isolates 5672-1 and 5672-3 were less virulent than other isolates tested, whereas 1080-3 and 1080-1 were more virulent than all tested isolates. Conidial production at 100% RH and 20 °C was higher than other %RH and temperatures tested. At 95% RH and temperatures of 30 and 15 °C, conidial production was limited to few isolates. Only 5672-5 produced conidia at 30 °C, and 5672-1, 5672-4, 5672-5 and the multispore isolate 5672 produced conidia at 15 °C, but no production of conidia was found for the multispore 1080 and its single spore isolates at these temperature and RH combinations. No conidial production was found at <95% RH. Variability in conidial germination, spore production and virulence of 5672 and 1080 and their single spore isolates tested will help in selection and development of these fungi for Sunn Pest, Eurygaster integriceps Puton management under temperatures and RH levels occurring in the Sunn Pest overwintering sites.
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