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Articles by Mohammed Amin Mohammed
Total Records ( 10 ) for Mohammed Amin Mohammed
  Amany H. Mansour , Tawfik R. Elkhodary , Rokia Anwar , Maha Ragab Habeeb and Mohammed Amin Mohammed
  Hepatocellular Carcinoma (HCC) is the major form of primary liver cancer and the third leading cause of cancer mortality worldwide. HCC is frequently diagnosed at an advanced stage, resulting in rather poor survival rates. CD133, a trans-membrane glycoprotein, is an important cell surface marker for both stem cells and Cancer Stem Cells (CSCs) in various tissues including the liver. CD133 has been used as the Tumor Initiating Cells (TICs) marker in HCC. Further identification and characterization of CSCs or TICs in HCC are necessary to better understand hepatic carcinogenesis. So, the objective of this study was to estimate the level of the specific markers as CD133 and transforming growth factor β (TGF-β) in HCC. Fifty peripheral blood samples were collected from HCC patients. The CD133 protein expression was analyzed by flow cytometry and TGF-β levels were assayed by ELISA. This study revealed a higher expression of CD133+cells in all HCC patients. The expression of CD133+cells was positively correlated with clinical, pathological and laboratory parameters. A mild to moderate positive correlation was found with tumor size, male gender, pathological grade, Hepatitis C virus and Alpha-fetoprotein and showed a strong positive correlation between age, TGF-β, Lymph node metastasis, portal vein tumor thrombosis and CD133+cells in peripheral blood. CD133+cells might represent true CSCs in HCC which could allow a better understanding of HCC initiation and progression, as well as possibly bear great therapeutic implications. Moreover, the multifunctional cytokine TGF-β plays a crucial role in the regulation of CD133 expression in HCC.
  A.H. Mansour , Mohammed Amin Mohammed , Rokia Anwar , M.E. Elzafrany and Nesreen Moustafa Omar
  There is growing evidence that the ABO blood group system may play a role in disease etiology. Studies conducted several decades ago, have demonstrated a relationship between inherited human ABO and Rhesus blood groups and risk of various malignancies. However, these findings are inconsistent and contradictory. The objective was to perform analysis of ABO and Rhesus blood antigens distribution among patients with various cancers including breast, hepatocellular, pancreatic, gastric, skin, lung cancers, leukemia and lymphoma in Dakahlia, Egypt and to assess their potential role in carcinogenesis. A total of 1131 cancer patients (age 63.8±9.3, female/male, 215/916) and another 1200 healthy controls (age 48.9±11.3, female/male, 348/852) were enrolled in this study. ABO blood groups were determined using Tube method and Gel method. The anti-TF IgG level and Von Willebrand factor were determined by enzyme-linked immunosorbent assay. The distribution of blood type A was significantly higher among cancer patients than among healthy controls (39.35 vs. 33.75%, p = 0.014), whereas, the distributions of other blood types were similar between cases and controls (p>0.05). Most cancers, especially gastrointestinal tumors were dominated by male gender independent of age. Blood group A was associated with significantly higher risk for malignancy including hepatocellular carcinoma, pancreatic and breast cancers while biliary and esophageal cancer risk was significantly associated with blood type B. We suggest that blood group A may elevate risk of cancer and may play a role in its development.
  Mohammed Amin Mohammed , Hany Shabana , Tarek Sheta , Nesreen Moustafa Omar and Soad Amin Mohammed
  Background and Objective: Vitamin D, considered as a systemic hormone and important immune modulator, has emerging roles in fibrogenesis, cell cycle arrest and cancer development. The objective of this study was to investigate the association between the Vitamin D Receptor (VDR) gene polymorphisms and hepatocellular carcinoma (HCC) development and severity in Egyptians with Chronic Hepatitis B (CHB). Methods: Two hundred and eighty-five adult consecutive outpatients were enrolled, of which 108 patients with CHB and HCC, 92 patients with CHB without HCC and 85 patients with HCC without CHB. Evaluation of clinicopathological characteristics of HCC was done. Genotyping of VDR gene at TaqI, BsmI, ApaI and FokI was also performed. Results: FokI TT genotype prevalence and T allele frequency were higher in HCC patients than those without HCC. Patients carrying FokI TT genotype had significantly higher risk for HCC (p <0.05) after using FokI CC genotype as a reference and adjusting other covariates including age, gender, CHB infection time, family history of cancer and serum ∞-fetoprotein levels. Also, patients carrying FokI TT genotype had advanced disease stage of cancer, liver cirrhosis, lymph node metastasis. Conclusion: Only the SNPs of VDR gene at FokI locus (C>T) could be used as a molecular marker predicting the risk and evaluating the severity of HCC in Egyptian patients infected with CHB. Polymorphism at FokI locus was associated with increased HCC susceptibility and has a significant role in the determination of its clinicopathological characteristics.
  Mohammed Amin Mohammed , Nesreen Moustafa Omar , Soad Amin Mohammed and Ahmed Mohammed Amin
  Background and Objective: MicroRNAs had been implicated in several malignancies. Abnormal circulating microRNA-1246 expression had been detected in HCC patients in an expanding number of studies. However, the information in literature describing the pertinent ramifications of miR-1246 in early-stage HCCs are rare and heterogeneous. This study was designed to assess the diagnostic accuracy of serum miR-1246 level in early-stage HCV-related HCC patients contrasted with chronic hepatitis C (CHC), liver cirrhosis (LC) and healthy control (HC). Materials and Methods: Two hundred HCV outpatients were doled out into 3 groups, HCC group (n = 100), CHC group (n = 30) and LC group (n = 70). Another hundred (100) age- and sex-matched healthy controls (HC) were likewise enlisted. The serum expression level of miR-1246 (by quantitative Real-Time PCR), AFP and prothrombin induced by vitamin K absence-II (PIVKA-II) were tested. Results: In HCC patients, in contrast to AFP, the serum expression levels of PIVKA-II and miR-1246 were statistically significantly increased discriminating HCC patients and early-stage HCCs not only from non-HCC patients (CHC, LC) yet additionally from HC. PIVKA-II and miR-1246, either individually or combined, had excellent diagnostic accuracies and performances as demonstrated by their ROCs and high AUCs >0.7. This serum over-expression positively correlated with the clinicopathological characteristics of both HCC and non-HCC patients. Conclusion: Serum miR-1246 level was significantly higher in HCC patients compared with non-HCC and HC and reliably discriminate early-stage HCV-related HCCs particularly when combined with PIVKA-II.
  Mohammed Amin Mohammed , Alaa Mahamad A. Hakeem El-Gamal , Nesreen Moustafa Omar , Abdelhadi M. Shebl , Amany H. Mansour , Sherin Mohamed Abd El-Aziz , Gamal Othman and Soad Amin Mohammed
  The major challenge in inflammatory bowel disease is to achieve a sensitive and specific non invasive diagnostic marker. Recently, S100A12 (Calgranulin C) have been established to be elevated in the feces of patients with IBD. The objective was to investigate the utility of fecal S100A12, in comparison to fecal Calprotectin and standard inflammatory markers, as a screening and distinguishing marker for IBD and Irritable Bowel Syndrome (IBS) in patients with chronic diarrhea. Stool samples were obtained from 173 individuals presenting with gastrointestinal symptoms requiring endoscopy. Fecal S100A12, fecal Calprotectin and serum S100A12 levels were measured and correlated to final diagnosis and standard tests (ESR, CRP, platelet count, albumin, perinuclear anti-neutrophil and antineutrophil cytoplasmic antibodies. Full colonoscopy with histopathological examination was performed. Patients diagnosed with IBD had elevated fecal S100A12 (median 49.7 mg kg-1) and Calprotectin (median 385 mg kg-1) levels compared with the patients without IBD (n = 35, S100A12: Median 4.6 mg kg-1, p<0.0001, Calprotectin: Median 30.5 mg kg-1; p<0.0001). Both the sensitivity and specificity of fecal S100A12 (cutoff 8 mg kg-1) for the detection of IBD were 93.91 and 97%, respectively whereas fecal Calprotectin (cutoff 35 mg kg-1) gave a sensitivity of 93.96% and a specificity of 84.2%. Both fecal markers were superior to the sensitivities and specificities of any standard inflammatory test. Both fecal S100A12 and Calprotectin are sensitive markers of gastrointestinal inflammation but fecal S100A12 provided exceptional specificity in distinguishing patients with IBD from patients without IBD.
  Mohammed Amin Mohammed , Nesreen Moustafa Omar , Amany H. Mansour , Sherin Mohamed Abd El-Aziz and Gamal Othman
  Vitamin D is an important immune modulator that has an emerging role in inflammatory and metabolic liver diseases. An association has been established between low levels of vitamin D and several adverse health outcomes including upper respiratory and enteric infections, viral hepatitis and HIV infections. It exerts protective effects during infections by up-regulating the expression of cathelicidin and β-defensin 2 in phagocytes and epithelial cells. Thus, vitamin D appears to have systemic antimicrobial effects that may be crucial in a variety of both acute and chronic illnesses. In the current study, 25-hydroxyvitamin D3 (25-OHD3) levels were compared among 75 patients with chronic hepatitis B virus infection (Group I), sixty naturally immunized individuals (Group II) and another sixty age and sex-matched healthy controls. Routine biochemical parameters like hepatitis markers, hepatitis B virus serology, hepatitis B virus DNA, 25-OHD3 and Parathormone levels were measured. Patients in group I had a significantly lower 25-OHD level compared with group II and controls (13.9±4.93 vs. 22.1±6.14 and 23.15±8.28 ng mL-1, respectively p<0.001). In contrast, patients in group I had a higher parathyroid hormone level compared with group II and control group (103.14±24.5 vs. 75.14±23.4 and 74.1±20.15 pg mL-1, respectively p<0.001). Also, 25-OHD levels were inversely correlated with hepatitis B virus DNA levels. The observed diminished 25-OHD levels in patients infected with hepatitis B virus may be an indicator of the viral replication status and portends a poor prognosis.
  Mohammed Amin Mohammed , Nesreen Moustafa Omar , Soad Amin Mohammed , Ahmed Mohammed Amin and Doaa Farouk Gad
  Background and Objective: As liver biopsy had multiple procedure-related complications, the introduction of reliable noninvasive tests for accurate discrimination of NASH and liver fibrosis in patients with NAFLD are mandatory. The aim was to elucidate the diagnostic value of fibrosis-4 index (FIB-4), Homeostasis Model Assessment for Insulin Resistance (HOMA-IR) and Cytokeratin-18 fragments (CK18-Fs) in the prediction of NASH and liver fibrosis. Materials and Methods: One hundred Egyptian patients with NAFLD selected from outpatient's clinics of Mansoura University Hospitals underwent histological examination through liver biopsy after approval and consent. The FIB-4, HOMA-IR, CK18-Fs (measured using a human ELISA Kit) and their combination in FICK-3 score were investigated for predicting NASH and liver fibrosis. Receiver operating characteristic (ROC) curves and multivariate logistic regression were analyzed. Results: In patients with NAFLD, the areas under the ROC were significantly high (AUC: 0.765, 0.700, 0.803, 0.835 for FIB-4, HOMA-IR, CK18-Fs, FICK-3 score, respectively, p = 0.05) displaying a highly statistically significant predictive ability for NASH. Significantly higher AUCs for these parameters were demonstrated predicting early-or advanced-stage liver fibrosis (AUC >0.7, p<0.01). Also, the combined FICK-3 score (the sum of FIB-4 >1.46, HOMA-IR >2.11 and CK18-Fs >307U L1) had highly significant predictive values for NASH and liver fibrosis and had the best diagnostic accuracy at a cutoff value of 1(AUC >0.8, p<0.001). Contrasted with other diagnostic scores, FICK-3 had the best diagnostic accuracy for detection of fibrotic NASH (AUC = 0.954, p<0.001) and positively correlated with the histological features of NAFLD. Conclusion: The new combination FICK-3 score was a reliable and significant predictor for NASH and liver fibrosis in NAFLD Egyptian patients.
  Mohammed Amin Mohammed and Nesreen Moustafa Omar
  Helicobacter pylori infection and its eradication may influence plasma levels and gastric production of some peptides, which can affect appetite. Ghrelin, an orexigenic hormone, is primarily produced in and secreted from oxyntic mucosa of the stomach. Ghrelin has been demonstrated to play a central role in appetite, food intake and energy homeostasis. As ghrelin production in human is exclusively gastric origin, it is conceivable and not surprising that any injury to the gastric mucosa will affect plasma ghrelin concentrations. To verify this hypothesis, a total of 135 adult consecutive individuals with normal body mass index including 84 H. pylori-infected and 51 H. pylori-negative subjects were included in a randomized controlled trial. Gastric ghrelin mRNA expression levels were measured in endoscopic biopsy specimens in both groups before and after H. pylori eradication. Also, plasma active n-octanoyl ghrelin and obestatin levels and ghrelin/obestatin ratio were measured in both groups before and after H. pylori eradication. The treatment group (44/84) received triple H. pylori eradication therapy for 7 days and followed up for 6 months. In contrast to obestatin, plasma and gastric ghrelin mRNA expression levels were significantly lower in H. pylori-infected subjects. H. pylori eradication significantly reversed these changes. The decrease in plasma ghrelin concentration in H. pylori-positive subjects was accompanied by depletion of ghrelin mRNA expression. These findings suggest that H. pylori induced chronic gastritis impair gastric ghrelin production and consequently the decrease in plasma ghrelin concentration.
  Mohammed Amin Mohammed , Rokia Anwar , Amany H. Mansour , Emad Elmasry and Gamal Othman
  Obesity is a chronic multi-factorial disease associated with serious increases in adiposity, morbidity and mortality rates. Ghrelin, leptin and adiponectin complexly interact to modulate appetite and mediate metabolic processes. The objective was to evaluate the effects of Bioenteric Intragastric Balloon (BIB) versus conservative therapy (diet and physical exercise) on plasma ghrelin, leptin, insulin and adiponectin in obese subjects. A total of 128 adult consecutive morbidly obese individuals were randomized into: BIB group comprised 84 patients treated with Intragastric Balloon and control group comprised 44 obese patients treated with conservative treatment only. In BIB group, insertion of balloon caused a significant reduction in body mass over a 6-months period compared with the control group. After one month, the levels of ghrelin increased significantly then gradually decreased, reaching the starting level three months after the removal of the balloon. Also, leptin level decreased significantly. In the control group, the corresponding of ghrelin and leptin levels remained relatively stable. In BIB group, adiponectin significantly increased as opposed to a transient increase in the control group. Conclusion: intragastric balloons in association with low-calorie diet are effective, safe, reversible, but short-lasting therapeutic endoscopic intervention in obese patients. The treatment with BIB causes significant changes in the levels of appetite-regulatory hormones beside a body mass reduction, changes in carbohydrate and lipid metabolism and improved co-morbidities.
  Mohammed Amin Mohammed , Nesreen Moustafa Omar , Abdelhadi M. Shebl , Amany H. Mansour , Emad Elmasry and Gamal Othman
  The correlation between celiac disease (CD) and type 1 diabetes (T1D) mellitus has been known for decades. However, the data assessing celiac disease prevalence in type 1 diabetic patients among Arab population especially in Egypt were scarce. This study assessed celiac disease prevalence and its genotypic profile in Egyptian type 1 diabetic patients and their non diabetic relatives and determined effects of gluten-free diet on diabetes control and anthropometry. A total of 500 outpatients {15-49 years; with type 1 DM (300; F/M: 168/132) and their non diabetic relatives (200; F/M: 91/109)} and another 300 age and sex matched healthy control were randomly enrolled and screened for CD by tissue transglutaminase (TTG) antibodies, HLA genotyping and distal duodenal biopsy. The demographic, clinical, anthropometric data and effects of gluten free diet were assessed. CD prevalence was 10.3, 2 and 0.3% in T1D, relative and control groups, respectively. The majority of CD patients (77.42%) carried the HLA-DQ2 in linkage with HLA-DRB1*03 alone (41.9%) or with other alleles (DRB1*01,*04,*07,*08,*09,*13). Eleven patients (35.48%) carried HLA-DQ8 either alone in three patients or with other alleles (DRB1*03,*07 in five and three patients respectively. Only one patient with positive TTG-IgG and normal histopathology had negative DQ2 and DQ8 but positive DRB1*7 and *11 haplotypes. CD is a frequent but commonly under-diagnosed among Egyptian T1D patients. HLA-DQ2 and DQ8 genotypes (in linkage with HLA-DRB1*3 and HLA-DRB1*4 alleles) responsible for CD development are highly prevalent in Egyptian CD patients with T1D.
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