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Articles by Mohammadzaman Basir
Total Records ( 2 ) for Mohammadzaman Basir
  Hadi Mahmoudi Moghaddam , Farnaz Etminan , Mohammadzaman Basir , Ehsan Mohajeri and Gholamreza Dehghannoudeh
  In this study, the possibility interactions of insulin with four non-ionic surfactants of polysorbates group using circular dichroism and fluorescence spectroscopy was investigated. The results showed that insulin structure is changed at pH 3 and 10 partially in comparison with pH 7. It was also was shown that polysorbates may cause a red shift of maximum emission fluorescence at all of the above pH values. Thermodynamic stability of insulin was determined in the presence of polysorbate group surfactants. The α-helix content of insulin exhibits a slight rising and thereby it causes an increasing in its free energy change (ΔG) and stability. The ΔG (H2O) of insulin was about 65.6 kj mol-1 in the presence of polysorbate 80 at pH 7. It was shown that polysorbate 80 increased the free energy and the α-helix content of insulin structure more than the others, so they could be considered as stabilizer in the formulations of protein drugs.
  Mandana Ohadi , Bagher Amir-Heidari , Mohammad Hassan Moshafi , Ali Mirparizi , Mohammadzaman Basir and Gholamreza Dehghan-Noudeh
  In the present study, biosurfactant production by immobilized Bacillus licheniformis PTCC 1320 in alginate beads has been studied. Alginate solution and bacterial suspension were mixed and stirred to get a uniform mixture. The slurry was taken into a sterile syringe and added drop wise into a stirring calcium chloride solution from a 5 cm height to form spherical beads. The beads were kept under agitation for about 2 h to harden and were then washed. Equal numbers of beads were charged to 250 mL erlenmeyer flasks each containing 50 mL of nutrient broth medium. Beads containing B. licheniformis placed in the nutrient broth medium were incubated in a shaker incubator at 30°C. Samples were collected at 24 h intervals and the parameters of biosurfactant production, such as surface tension, emulsification activity and foam production were studied. Results showed that B. licheniformis entrapped in calcium alginate beads is able to preserve its viability and produce biosurfactant as a secondary metabolite. Maximum biosurfactant production was achieved during the first 24 h.
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