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Articles by Mohammad Rafiq Wani
Total Records ( 1 ) for Mohammad Rafiq Wani
  Sonu Goyal , Mohammad Rafiq Wani and Samiullah Khan
  Background and Objective: The nature and extent of genetic variability available within the species forms the basis for effective selection. Present study was aimed at to explore the possibility of inducing genetic variability in M2 and M3 generations of urdbean following mutagenesis with individual and combination treatments of gamma rays and ethyl methanesulfonate (EMS) for selecting enviable early flowering and maturing lines for late spring season. Materials and Methods: Physically, seeds of two varieties of urdbean were irradiated with 200 and 300 Gy doses of gamma rays at NBRI, Lucknow. For chemical treatments, seeds were treated with 0.2 and 0.3% of EMS for 6 h and for combination treatments, dry seeds of each variety were firstly irradiated with 200 and 300 Gy doses of gamma rays followed by the treatment with 0.2% of EMS. The mutagen treated seeds were sown in complete randomized block design to raise M1 generation. The M1 seeds were sown in plant progeny rows to grow M2 generation. The 10 M2 progenies showing significant negative deviation in mean values from their respective controls particularly for flowering and maturity were selected to raise M3 generation. Analysis of variance was performed to assess the extent of induced variation for both the traits. Results: Data for days to flowering and maturity in M3 generation had resulted in reducing the flowering and maturity period by more than four days after mutagenic treatments in both the varieties. Combination treatments were found to be more effective in reducing the flowering and maturity period than the individual treatments of gamma rays and EMS. Genotypic coefficient of variation (GCV), heritability (h2) and genetic advance (GA) increased manifold in the treated population. Conclusion: The quantitative traits (flowering and maturity) exhibited higher genetic variability in M2 as compared to M3 generation indicating that potential gain could be achieved through selection in early (M2) generation.
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