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Articles by Mohammad Taghi Akhi
Total Records ( 4 ) for Mohammad Taghi Akhi
  Mohammad Asgharzadeh , Saber Yousefee , Hossein Samadi Kafil , Mohammad Reza Nahaei , Khalil Ansarin and Mohammad Taghi Akhi
  To investigate the genetic variation among Mycobacterium tuberculosis isolates from East and West Azarbaijan provinces of Iran and to evaluate the manner of recent transmission of tuberculosis (TB), we performed IS6110-based restriction fragment length polymorphism analysis of isolates. Restriction fragment length polymorphism (RFLP) typing performed on 165 culture-positive specimens from East and West Azarbaijan. Using IS6110 as a probe, Mycobacterium tuberculosis strains assigned to clusters based on identical DNA fingerprints. Rates of patients have clustered were 27.68% in East and 30.19% in West Azarbaijan. There was not statistically significant differences in clustering of patients in two provinces (p = 0.4533) but infection with Mycobacterium tuberculosis in males and females in two provinces were different (p = 0.0048). In East Azarbaijan there was not difference in transmission of tuberculosis between males and females, also in males and females belonged to clusters we couldn’t find statistical difference (p = 0.1833). The rate of active transmission of TB in West Azarbaijan was slightly more than East Azarbaijan. It can be due to different factors such as poor economic and less developed condition in West Azarbaijan.
  Mohammad Taghi Akhi , Mohammad Reza Nahaei , Mojtaba Nikbakht and Mohammad Asgharzadeh
  The aims of present investigation were to study the nasal carriage rate of MRSA in hospital staff and in-patients, determination of antibiotic resistant patterns of nasal and clinical MRSA isolates and typing of MRSA isolates by RAPD- PCR. Two hundred and six S. aureus isolates were recovered from clinical specimens and noses of 460 staff and in-patients admitted in Imam Khomeini and Pediatrics hospitals by standard methods during 6 months (2004-2005). Disk agar diffusion (using 13 antibiotics disks) and oxacillin agar screening methods for detection of MRSA isolates were performed according to CLSI. PCR was also used to amplify a 310 bp sequence from S. aureus genome (mecA gene) for detection of MRSA isolates. RAPD-PCR was carried out for fingerprinting of MRSA isolates genome. MRSA isolates were resistant up to 11 antibiotics. All of the MRSA isolates were resistant to penicillin, but sensitive to vancomycin. Of 206 S. aureus isolates, 77 MRSA isolates were detected using disk agar diffusion and oxacillin agar screening methods. In contrast, 80 isolates were detected as MRSA by amplification of mecA sequence. In RAPD-PCR experiments, 43 different RAPD patterns were obtained from our MRSA isolates. Nasal carrier rate of S. aureus was 34.7% and MRSA isolates were high (38.8%) in our hospitals. This study revealed high rate of MRSA, that infected patients and MRSA nasal carriers (staff and in-patients) were the main source of transmission and infection, therefore effective control measures are necessary to avoid nosocomial infection outbreaks.
  Mohammad Asgharzadeh , Saber Yousefee , Mohammad Reza Nahaei , Mohammad Taghi Akhi , Khalil Ansarian and Hossein Samadi Kafil
  IS6110-based DNA fingerprinting is currently the most widely used genetic marker for differentiating among Mycobacterium tuberculosis strains. To evaluate the DNA polymorphism among Mycobacterium tuberculosis strains and to determine if there is matching of IS6110 fingerprints representing recent transmission of tuberculosis. Totally one hundred and sixty five isolates of M. tuberculosis (53 from West Azarbaijan and 112 from East Azarbaijan) were analyzed by IS6110 restriction fragment length polymorphism fingerprinting. Isolates having identical RFLP patterns were considered a cluster. The average number of IS6110 copies per strain was 7.3 and ranged from 0 to 17 among the M. tuberculosis isolates. The IS6110-DNA patterns from these isolates were highly polymorphic. In conclusion 123 patterns were observed which 16 patterns were shared by 47 isolates (30.52%). Most strains (93.62%) had multicopy patterns and only 3 of clustered isolates had less than six IS6110 copies. In our study increased clustering was observed with isolates from male patients. RFLP analysis of 154 isolates of M. tuberculosis showed a considerable diversity, suggesting that most patients were infected with unique strains, probably resulted from reactivation of the latent infection.
  Mohammad Reza Nahaei , Yaeghob Sharifi , Mohammad Taghi Akhi , Mohammad Asgharzadeh , Mehrnaz Nahaei and Ebrahim Fatahi
  The aim of this study was to detect cytotoxin-associated gene A (cagA) and vacuolating cytotoxin gene A (vacA) genotypes of Helicobacter pylori and to study their relationships to the associated diseases. In the present analytical descriptive study H. pylori isolates were collected from 150 patients who underwent gastro duodenoscopy in Imam Khomeini Hospital of Tabriz, Iran. Of the patients 76 (50.7%) were males and 74 (49.3%) were females. The patients were divided into two groups. Group I consisted of 117 (78%) Non-Ulcerative Dyspepsia (NUD) patients and group II consisted of 33 (22%) Peptic Ulcer Disease (PUD) patients. Extracted DNA of H. pylori isolates were subjected to PCR tests to detect cagA, signal (s) and middle (m) regions of vacA genotypes. The designed primers revealed the presence of cagA gene in 125 (83.3%) of the isolates. Regarding vacA signal sequences 99 (66%) of our isolates revealed s1 type. The proportion of s1a, s1b and s1c subtypes were 76/150 (50.7%), 7/150 (4.7%) and 16/150 (10.6%), respectively while 40/150 (26.7%) presented as s2 type. In further analysis of the m region of vacA, m1 and m2 subtypes were detected in 49/150 (32.7%) and 81/150 (54%) of the isolates, respectively. The m1 subtype were further divided into m1a [41/49 (83.7%)] and m1b [(8/49 (16.3%)]. Thirty one isolates (20.7%) showed more than one vacA alleles in a single patient. Our results showed that isolates carrying the cagA gene were higher in PUD group than in NUD group, but did not substantiate statistically the role of cagA as a marker influencing increased virulence (p>0.05). Present findings also showed that s1 and s2 subtypes of vacA gene are markers which differentiate between PUD and NUD groups.
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