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Articles by Mohammad Javad Raee
Total Records ( 2 ) for Mohammad Javad Raee
  Younes Ghasemi , Alireza Ebrahiminezhad , Sara Rasoul-Amini , Gholamreza Zarrini , Mohammad Bagher Ghoshoon , Mohammad Javad Raee , Mohammad Hossein Morowvat , Farshid Kafilzadeh and Aboozar Kazemi
  Purified L-asparaginase II from Escherichia coli has been supplied and employed in the acute leukemia and other malignant neoplasms chemotherapy. L-asparaginase II gene (ansB) in E. coli is under regulation and certain conditions is needed for expression of this gene. In this investigation ,the various concentrations of modified M9 medium ingredients and various carbon source were tested to optimize the medium for expression and identification of L-asparaginase in E. coli. Finally a semi-quantitative plate assay for L-asparaginase producing Escherichia coli is reported.
  Younes Ghasemi , Sara Rasoul-Amini , Mohammad Hossein Morowvat , Seyed Bagher Mosavi Azam , Shadman Shokravi , Abdolali Mohagheghzadeh , Mohammad Bagher Ghoshoon and Mohammad Javad Raee
  A unicellular microalga, Oocystis pusilla, was isolated from paddy-field and applied in the biotransformation experiment of hydrocortisone (1). This strain has not been previously tested for steroid bioconversion. Fermentation was carried out in BG-11 medium supplemented with 0.05% substrate at 25 °C for 14 days incubation. The products obtained were chromatographically purified followed by their characterization using spectroscopic methods. 11β, 17α, 20β, 21-tetrahydroxypregn-4-en-3-one (2), 11β, 17β-dihydroxyandrost-4-en-3-one (3) and 11β-hydroxyandrost-4-en-3, 17-dione (4) were the main byproducts in the hydrocortisone bioconversion. Bioreaction characteristics observed were 20-ketone reduction for accumulation of compound 2 and side chain degradation of the substrate to prepare compounds 3 and 4. Time course study showed the accumulation of the product 2 from the second day of the fermentation and 3 as well as 4 from the third day. All the metabolites reached their maximum concentration in seven days. Optimum concentration of the substrate, which gave maximum bioconversion efficiency, was 0.5 mg mL-1 in one batch. Growth was not influenced by the addition of steroid substrate. Biotransformation was completely inhibited in a concentration above 2.0 mg mL-1.
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