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Fatty acid analysis in cotton seedlings following waterlogging showed changes in both their levels and relative composition. This treatment caused a marked increase in the amount of esterified fatty acids. Waterlogging also enhanced the synthesis of a set of low molecular weight compounds and suppressed the synthesis of all the “normal” fatty acids. A significant increase in free fatty acids was also observed.
Heat-hardening treatments
indicates that B.rhizobium japonicum can grow better at high
temperatures. During first 12h of incubation (after hardening at 40°C)
the rhizobia sometimes grew less well than the controls, but growth
then increased more quickly up to 36h of incubation compared to controls.
A small decrease in cell population was measured after 48h, however,
similar to that in the control. At 45°C, the heat-hardened rhizobia
grew similar to the controls during the first 12h, then a sharp increase
was found after 36h. Growth was then much better in the hardened cultures
than controls. Same time at 40°C, the pH declined sharply after
12h and remained stable on further incubation. However, the pH of the
culture which was incubated at 45°C did not decline during the
first 24h of incubation and only a very slow decline was found after
that. Similar results were found in the cultures which had been hardened
at 45°C. In cultures which were incubated at 40°C, the pH
decreased slightly after 12h of incubation and became stable on further
incubation. At 45°C, decrease in the pH was not found up to 24h
and then a small decline take place with increasing incubation periods.
