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Articles by Mohammad Asgharzadeh
Total Records ( 3 ) for Mohammad Asgharzadeh
  Behnam Mohammadi Ghalehbin , Mohsen Arzanlou , Esmaeel Fallah , Abdol Hassan Kazemi and Mohammad Asgharzadeh
  Cryptosporidium parvum is a protozoan parasite that is now recognized as one of the leading causes of diarrhea in cattle. Surface waters contaminated with human and animal feces serve as main source for epidemic spread of Cryptosporidium parasites. In this study, we used a small-subunit rRNA-based Pcr-restriction Fragment Length Polymorphism (RFLP) technique to determine the prevalence and to characterize species of Cryptosporidium parasites in cattle`s in Ardabil city in Iran. Among 107 samples examined, 19 samples showed positive results. Restriction pattern analysis showed C. andersoni as the most common species with 14 cases; followed by C. parvum, bovine genotype, with 5 cases. Our results confirm that zoonotic transmission can be occur in the study region.
  Mohammad Asgharzadeh , Mansour Khakpour , Taghi Zahraei Salehi and Hossein Samadi Kafil
  The aim of present study was to determine the genotypes of isolates from East Azarbaijan province by this method. We performed (MIRU-VNTR) analysis of strains, isolated from 127 patients during a period of September 2002 to March 2003 in tuberculosis centers of the province. Among 127 isolates, we found 93 distinct MIRU-VNTR patterns, including in 21 clustered patterns and 72 unique patterns from isolated strains. The discriminatory power of MIRU-VNTR typing in present study was high (HGDI = 0.9932) for isolates. In clusters similar patterns of Nakhichevanees patients and Iranian patients was revealed in three clusters which showed Nakhichevanees patients referred to tuberculosis centers of province could be a source for transmission of tuberculosis. Tuberculosis in this province is relatively in good condition. The allelic diversity of our samples was lower than previous studies. These results indicate that MIRU-VNTR can be a useful and first line tool for studying genetic diversity of M. tuberculosis isolates in regional setting such as East Azarbaijan province of Iran.
  Habib Onsori , Mohammad Ali Hosseinpour , Sheideh Montaser-Kouhsari , Mohammad Asgharzadeh and Abbas Ali Hosseinpour
  Hemophilia A is an X-linked congenital bleeding disorder caused by factor VIII deficiency. The factor VIII gene is on the long arm of the X chromosome at Xq28 spans 186 kb and consists of 26 exons. In this study to identify defects in the factor VIII gene, Single-Stranded Conformation Polymorphism (SSCP) analysis was used. A novel missense mutation due to T → C transition at codon 153 (TGC) of the factor VIII gene which replace a cysteine with an arginine residue, was found in a patient of North-Western of Iran with sever hemophilia A. Direct sequencing of the amplified fragment was performed to confirm the mutation. This study shows that we can use of Polymerase Chain Reaction (PCR) and silver staining of SSCP methods for detecting most of the point mutations causative hemophilia A.
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