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Articles by Mohammad Ali Faramarzi
Total Records ( 4 ) for Mohammad Ali Faramarzi
  Eshrat Gharaei-Fathabad , Mojtaba Tabatabaei Yazdi , Seyed-Naser Ostad , Shadman Shokravi , Zargham Sepehrizadeh , Mohammad Ali Faramarzi and Mohsen Amini
  A total of 100 supernatants and methanol extract samples obtained from three weeks’ incubation of 50 different isolate strains of cyanobacteria with paddy-fields origin were investigated for their cytotoxicity properties. Fifteen supernatants and 10 methanol extract samples exhibited cytotoxic activity against Balb-C cells while one methanol extract sample possessed a cytotoxic effect against five different cell lines including HeLa, Vero, Caco-2, HepG-2 and CHO more than vincristine, 5-fluorouracil and methotrexate when used at their IC50 concentrations. The later potent extract was achieved from the cyanobacterium strain GT-319. It was characterized as Nostoc piscinale Kützing ex Bornet and Flahault 1888 according to the morphological studies. Identification of the species was performed following molecular taxonomy by 16S rRNA sequencing. This is the first report on the cytotoxic activity of N. piscinale GT-319.
  Mohammad Ali Faramarzi , Mojtaba Tabatabaei Yazdi , Mohsen Amini and Abbas Shafiee
  The whole cells of Acremonium strictum transformed prednisolone at its side chain to produce two steroid compounds. 21,21-Dimethoxy-11β-hydroxypregn-1,4-dien-3,20-dione was the main metabolite which its production has not been previously reported using microbiological means. This metabolite together with a hydroxylated derivative, 11β-hydroxyandrost-1,4-dien-3,17-dione, were purified with preparative TLC followed by their identification through 1H, 13C NMR and other spectroscopic data. Best fermentation condition was found to be 5 day incubation at 25 °C and pH value of 6 according to TLC profiles. Optimum concentration of the substrate, which gave maximum bioconversion efficiency, was 1 mg mL-1 in one batch. Biotransformation was completely inhibited in a concentration above 5 mg mL-1.
  Hamid Reza Monsef-Esfahani , Mohammad Ali Faramarzi , Venus Mortezaee , Mohsen Amini and Mohammad Reza Rouini
  A High-Performance Liquid Chromatography (HPLC) method was developed for determination of harmine, harmaline, harmol and harmalol in the extract of Peganum harmala seeds. The sample preparation was performed using liquid-liquid extraction. Chromatographic separation was achieved with a Tracer Excel 120 ODSA (150x4.6 mm) column, using a mixture of potassium phosphate buffer (10 mM, pH 7.0): acetonitrile (100:30; v/v) as mobile phase, in an isocratic mode at 1.5 mL min-1. UV detection (λ = 330 nm) was used. The calibration curves were linear (r2>0.998) in the concentration range of 0.5-20 μg mL-1 for all analytes. The lower limit of quantification for all analytes was 0.5 μg mL-1. The within and between day precisions in the measurement of QC samples at three tested concentrations were in the range of 0.6-10.2% for all analytes. The HPLC method is able to measure the harmala alkaloids in the plant extract. The method has suitable reproducibility, sensitivity and resolution for routine and accurate use with UV detection.
  Zahra Moradpour , Maryam Torshabi , Mohammad Ali Faramarzi , Mojtaba Tabatabaei Yazdi , Younes Ghasemi , Hoda Jahandar , Nadia Zolfaghary and Gholamreza Zarrini
  The ability of Nostoc ellipsosporum PTCC 1659, a cyanobacterium strain, for biotransformation of androst-4-en-3,17-dione (AD) was studied. Fermentation was performed in BG-11 medium supplemented with 0.05% AD at 25°C for seven days incubation. The single metabolite obtained was purified using chromatographically methods and characterized as testosterone on the basis of its spectroscopic features. Bioreaction characteristic observed was 17-carbonyl reduction. Time course study showed the accumulation of the product from the third day of the fermentation and reached to the maximum in the seventh day. Production of testosterone was not affected by aeration. Continuously illumination or 16 h light/8 h dark has no effect on the transformation yield. Optimum concentration of the substrate, which gave maximum bioconversion efficiency, was 0.5 mg mL-1 in one batch. Biotransformation was completely inhibited in a concentration above 2.0 mg mL-1.
 
 
 
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