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Articles by Mohamed El Mahjoub
Total Records ( 6 ) for Mohamed El Mahjoub
  Naima Boughalleb and Mohamed El Mahjoub
  Isolation from infected watermelon samples has demonstrated the presence of some fungal complex including Fusarium oxysporum, F. solani, Rhizoctonia bataticola, R. solani, Monosporascus cannonballus, Pythium ultimum and P. echinulatum. These fungal species are different in their distribution among the infected plants and areas surveyed. However, F. oxysporum and F. solani are always predominant. These results showed the almost-presence of the two Fusarium disease of watermelon: Fusarium wilt to F. oxysporum f. sp. niveum and the collars and/or root rot caused by F. solani f. sp. cucurbitae which are the primary fungi of sudden wilt. Analysis in main component of isolation frequency of different fungi showed that their association in homogeneous groups seemed not affected by geographic origin and surveyed year.
  Fakher Ayed , Mejda Daami-Remadi , Hayfa Jabnoun-Khiareddine , Khaled Hibar and Mohamed El Mahjoub
  Four fungicides, which active ingredients are hymexazol, azoxystrobin, fludioxonil and quinoline, are tested in vitro and in vivo against five isolates of Fusarium oxysporum f. sp. tuberosi, causal agent of potato Fusarium wilt. Pathogen radial growth noted on PDA amended with fungicides after incubation for four days at 25°C varied upon pathogen isolates. Mycelial growth of all pathogen isolates was significantly inhibited by fungicide PDA amendment; inhibition obtained by hymexazol treatment reached 77% comparatively to untreated control. All chemical fungicides reduced disease incidence compared to the untreated control. Hymexazol and azoxystrobin are the most active during all the bioassay period. Whereas, fludioxonil and quinoline showed a limited effect in controlling Fusarium wilt development.
  Khaled Hibar , Mejda Daami-Remadi , Hayfa Jabnoun-Khiareddine and Mohamed El Mahjoub
  In this study, the efficacy of grafting two tomato cultivars onto two rootstocks was examined in growth chamber and in greenhouse conditions. The rootstock cultivars Beaufort F1 and He-Man F1, already known and confirmed as resistant to Fusarium oxysporum f. sp. radicis-lycopersici, were evaluated during two crop seasons under greenhouse heated with geothermal water in South Tunisia. The cv. Durintha F1 showed the best plant growth, fruit yield and fruit quality when grafted onto Beaufort F1; while cv. Bochra F1 gave the best results when grafted onto He-Man F1. This study demonstrated that grafting tomato cultivars onto Fusarium resistant rootstocks is one of the best alternatives for controlling Fusarium crown and root rot of tomato.
  Khaled Hibar , Mejda Daami-Remadi , Hayfa Jabnoun-Khiareddine and Mohamed El Mahjoub
  Fusarium crown and root rot of tomato (Lycopersicon esculentum) caused by Fusarium oxysporum f.sp. radicis-lycopersici is a new damaging disease of greenhouse crops in Tunisia. This pathogen was detected in Southern Tunisia, where temperature is usually high. Thus, temperature effect on its development was studied in vitro, on mycelial growth and in vivo, on disease incidence. Temperature effect on mycelial growth of Fusarium oxysporum. sp. radicis-lycopersici, evaluated on Potato Dextrose Agar (PDA) media, revealed that this pathogen grows well at temperatures ranged from 20 to 30°C. However, the optimum of mycelial growth was recorded at 25°C (growth speed exceeded 13 mm D-1). On disease incidence, temperature effect was evaluated by breeding inoculated plants under two thermal conditions: 19 and 29°C. Damages observed, after an incubation period ranged between 30 and 50 days, were more important at 19°C than that at 29°C. Indeed, at 19°C, disease incidence exceeded 96% and it was always more than 70%. At 29°C, however, this value didn`t exceed 60% and this by using two inoculation methods.
  Mejda Daami-Remadi , Fakher Ayed , Hayfa Jabnoun-Khiareddine , Khaled Hibar and Mohamed El Mahjoub
  Four Bacillus sp. isolates were individually essayed against Fusarium solani, F. oxysporum f.sp. tuberosi, F. graminearum and F. sambucinum following an in vitro dual culture plate technique and in vivo pre-inoculation tuber treatment. All tested bacterial isolates significantly reduced radial mycelial growth of Fusarium spp., on PDA after 3 days of incubation at 25°C, comparatively to the untreated controls. Light microscopic studies of antagonist x Fusarium spp. in vitro interaction showed several hyphal abnomalies traduced particularly by lesser mycelium density, severe hyphal lysis, lesser sporulation, mycelial cords formation and early chlamydospores induction observed only at the confrontation zone. Potato tubers, cv. Spunta, treated by Bacillus sp. 24 h before individually inoculation by Fusarium species, showed reduced dry rot development after 21 days of incubation at 25-27°C comparatively to untreated controls.
  Hayfa Jabnoun-Khiareddine , Mejda Daami-Remadi , Khaled Hibar , Fakher Ayed and Mohamed El Mahjoub
  In many surveys in the Tunisian Sahel, several isolates of Verticillium have been collected from vegetable crops showing symptoms of Verticillium wilt. These isolates were identified as V. dahliae, V. albo-atrum and V. tricorpus on the basis of their morphological and cultural characteristics. Four isolates of V. dahliae, two isolates of V. albo-atrum and two isolates of V. tricorpus were examined for their mycelial growth at different incubation temperatures and their virulence against tomato and eggplant cultivars. Seedlings at the three leaf stage were root-dipped in a conidial suspension (107 conidia mL-1) and maintained in the glasshouse at 21-25°C. Disease severity was assessed through the index of leaf damage and plant height, recorded eight weeks after inoculation. Growth test showed that the majority of Verticillium isolates were able to grow from 10 to 30°C. All tomato and eggplant seedlings exhibited typical Verticillium symptoms compared to the non-inoculated control. The index of leaf damage, as well as plant height, differs significantly depending on cultivars and isolates. V. albo-atrum and V. tricorpus were moderately to highly pathogenic against eggplant and tomato plants. V. dahliae was the most aggressive against these hosts, showing a high level of pathogenicity to both eggplant and tomato plants.
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