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Articles by Mohamed E. Ahmed
Total Records ( 6 ) for Mohamed E. Ahmed
  Mohamed E. Ahmed and Imadeldin E. Aradaib
  A 14 year old female was admitted to Elshab Medical Teaching Hospital, Khartoum, Sudan. As informed by the father, there was a history of cough for 2 months, chest pain for 2 months and breathlessness for 3 weeks. The X-ray and MRI revealed the presence of bilateral pulmonary hydatid cyst. The patient was referred to thoracic surgery unit for thoracotomy of the left side of the chest. Because of the deteriorating condition of the patient, thoracotomy was initially performed in the right side of the chest to remove the giant hydatid cyst. A week later, thoracotomy was also performed to remove a ruptured pulmonary cyst from the upper lobe of the left lung, which was found infected with secondary bacterial organism. Molecular characterization rebelled that the cyst is of genotype 6 (G6) strain as detected by polymerase chain reaction (PCR)-based assay.
  Haitham A. Albir , Suliman M. ElSanousi , Tarig G. Eldawi , Mohamed E. Ahmed and Imadeldin E. Aradaib
  In the present study, conventional bacterial isolation, bacteriophage-based assay (FAST Plaque TB) and Polymerase Chain Reaction (PCR) were evaluated for detection of Mycobacterium tuberculosis complex. A total of 47 mycobacterial isolates consisting of 38 isolates of Mycobacterium tuberculosis complex and 9 isolates of mycobacteria other than M. tuberculosis complex were used in this study. In addition, nine reference strains of Mycobacterium consisting of 7 Mycobacterium tuberculosis, one strain of Mycobacterium flavescens, one srtrain of mycobacterium duvalii were also used in this study. Conventional isolation is laborious, cumbersome and time consuming where it takes as long as 2 months for definitive diagnosis. The phage assay is sensitive and it takes only two working days. PCR is a rapid assay and definitive diagnosis of tuberculosis infection could be made possible within the same working day. The described bacteriophage-based assay could be used as rapid, sensitiveand specific method to support the currently available conventional methods used for detection of Mycobacterium tuberculosis in developing countries.
  Safa A. Sherfi , Hamid A. Dirar , Badr E. Hago , Mohamed E. Ahmed , Hassan A. Musa , Hassan Abu Aisha and Imadeldin E. Aradaib
  The potential of the Polymerase Chain Reaction (PCR), as a means of detecting Escherichia coli (E. coli) DNA in suspected environmental samples, was studied. Using a pair of outer primers P1 and P2, selected from uidA gene, which encodes E. coli glucuronidase, the PCR-based assay resulted in amplification of a 486 base pair (bp) PCR product. E. coli strains from different environmental sources including recycled and drinking water as well as stagnant water were detected by this nested PCR-based assay. Amplification products were visualized on ethidium bromide-stained agarose gel. The sensitivity of the PCR assay was 100 fg of bacterial DNA with ethidium bromide-stained agarose gels. Using a pair of internal (nested) primers P3 and P4, the nested PCR produced a 186 bp PCR product. The nested PCR increased the sensitivity of the PCR assay by 1,000 times and specific PCR products were detected from 0.1 fg of bacterial DNA. Amplification product was not detected when the nested PCR-based assay was applied to DNA from other related bacteria including, Salmonella, Pseudomonas and Proteus or nucleic acid-free water. Application of this nested PCR-based assay to environmental samples resulted in direct detection of E. coli DNA from sewage water, tap water, drinking water at Shambat Campus, University of Khartoum, Sudan. This nested PCR-based assay should provide a rapid, sensitive and specific assay for direct detection and quantification of E. coli in environmental samples suspected to contain the organism.
  Mohamed E. Ahmed and Imadeldin E. Aradaib
  A 14 year old female was admitted to Elshab Medical Teaching Hospital, Khartoum, Sudan. As informed by the father, there was a history of cough for 2 months, chest pain for 2 months and breathlessness for 3 weeks. An intercostals drainage tube was administered to alleviate the condition and to relief pneumothorax. The patient was referred to thoracic surgery unit for thoracotomy of the left side of the chest. The second X-ray and MRI revealed the presence of a large hydatid cyst in the lower lobe of the right lung. The hydatid cyst was situated at the junction of the ventrolateral aspect of the upper lobe and dorsolateral aspect of the lower lobes of the right lung. Because of the deteriorating condition of the patient, thoracotomy was initially performed in the right side of the chest to remove the giant hydatid cyst. A week later, thoracotomy was also performed to remove a ruptured pulmonary cyst from the upper lobe of the left lung, which was found infected with secondary bacterial organism. An incidental cyst was also observed in the liver. Thus, this patient represents a case of multiple hydatid cysts. Conventional bacteriological examination revealed isolation and identification of Pseudomonus stutzeri from the infected hydatid cyst. Molecular characterization revealed that the cyst is of genotype 6 (G6) strain of Echinococcus granulosus as detected by polymerase chain reaction (PCR)-based assay.
  Mohamed E. Ahmed and Imadeldin E. Aradaib
  A child was admitted to Elshab Medical Teaching Hospital, Khartoum, Sudan. The patient had a history of cough, chest pain for 2 months and breathlessness for 3 weeks. X-ray, Computed Tomography (CT) scan and Magnetic Resonance Image (MRI) revealed the presence of a large rounded hemogenous well circumscribed nodular opacity in the right lung and hydrothorax in the left side of the chest. On thoracotomy, a ruptured hydatid cyst was found invaded by secondary bacterial infection leading to empyema of the left side of the chest. The infected ruptured hydatid cyst was removed and empyema was taken care of by enucleation of the cyst and decortication of the pleura and removal of the puss. The surgically removed cyst was submitted to the diagnostic laboratory for parasitological and microbiological examinations. Parasitological examination revealed the presence of a few number of protoscolices associated with the inner wall of the hydatid cyst. Conventional bacterial isolation and characterization revealed the presence of gram negative bacteria, which was further identified as Pseudomonus stutzeri. The antibiotic sensitivity test showed inhibitory zones of bacterial growth around a number of antibiotic discs with highest sensitivity being against tetracycline. To our knowledge, this is the first report on thoracic empyema caused by this organism in childhood in the Sudan.
  Mohamed E. Ahmed , Imadeldin E. Aradaib , AbdelRahim E. Karrar , Badr E. Hago and Safa A. Sherfi
  Two patients (adult females) were admitted to Ahmed Gasim Medical Hospital, Khartoum North, Sudan, with history of severe combined mitral regurgitation and stenosis. The patients were referred to the thoracic surgery unit for mitral valvectomy and valve replacement. Both patients received early empirical prophylactic antibiotic therapy half an hour before as well as post operatively. However, they developed fever shortly after the operation. Blood samples were collected and submitted to the diagnostic laboratory for conventional and molecular microbiological examinations. Conventional bacteriological examination revealed that blood cultures were negative for any bacterial growth. However, the Polymerase Chain Reaction (PCR) detected a 486 bp PCR product specific for E. coli. The identity of the nucleic acid sequence was confirmed by nested amplification of a 186 bp PCR product from the primary PCR product. The scientific data presented in this study indicated that PCR provides a rapid method for detection of E. coli during bacteraemia, irrespective of their viability. However, conventional bacterial isolation methods failed to diagnose E. coli infection in patients receiving high doses of antibiotics.
 
 
 
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