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Articles by Mohamed A. Abdalla
Total Records ( 3 ) for Mohamed A. Abdalla
  Mohamed A.M. Yousof , Imadeldin E. Aradaib , Tamadour M. Abdalla , AbdelRahim E. Karrar , Kamal E.E. Ibrahim , Mohamed A. Abdalla and Abdelrahim M. Hussein
  In this study, a nested Reverse Transcriptase (RT) polymerase chain reaction (RT-PCR)-based assay, for detection of Newcastle Disease Virus (NDV) Ribonucleic acid (RNA) in clinical samples from experimentally infected chicks, was evaluated. The clinical samples used included, blood, tracheal, cloacal, liver, spleen, heart, lung, kidney and brain. The nested RT-PCR was performed in two amplification steps. In the first step, a pair of primers (nd and nd ) was used to amplify a 356 bp specific region in the F gene of NDV. In the second step, a nested pair of primers (nd and nd ) was employed to produce 216 bp amplification products, internal to the annealing sites of primers nd and nd . The 356 bp PCR products were amplified only from lung homogenate, cloacal and tracheal tissues, kidney, heart and brain. However, the 216 bp nested amplification was detected in all tissue samples collected from experimentally infected chicks. The nested amplification confirmed the identity of the first amplified product and increased the sensitivity of RT-PCR assay. RNA samples extracted from Infectious Bursal Disease Virus (IBDV) and Infectious Bronchitis virus (IBV) or total nucleic acid extracted from blood of non infected birds failed to demonstrate the primary or the nested PCR products. The described nested RT-PCR assay provide reliable, rapid, sensitive and specific diagnostic assay for detection of an outbreak of NDV infection among susceptible Birds.
  Salah , M. M. Elamin , H. Salah Idris , Mohamed A. Abdalla , Badr E. Hago , Mohammed M. Salih and Rihab Omer
  A complementary DNA (cDNA) probe, derived from genome segment 6 (NS1) of epizootic hemorrhagic disease virus (EHDV) serotype 1 (EHDV-1), was synthesized by polymerase chain reaction (PCR) and evaluated for detection of Sudanese EHDV serogroup. A pair of primers (P1 and P2) was designed from NS1 genome segment of EHDV-1 and used for synthesis of a 387-bp cDNA probe. The cDNA probe hybridized with dsRNA from Sudanese EHDV serotypes including EHDV serotypes 4 and an untyped isolate designated (EHDV-318). However, dsRNA from blue tongue virus serotype 1, 2, 4 and 16 failed to hybridize with the cDNA probe. The result of this study indicated that, the developed cDNA probe could be used for rapid detection and differentiation of EHDV serogroup in cell culture.
  Abdel Rahim E. Karrar , Mohamed A. Abdalla , Ali M. Majid and Mohammed M. M. Salih
  A reverse transcriptase (RT) polymerase chain reaction (RT-PCR)-based assay, for detection of African isolates of palyam virus ribonucleic acid (RNA) in cell culture, was developed. A pair of oligoribonucleotide primers (pal1 and pal2), selected from genome segment 3 of Chuzan virus, an isolate of the palyam serogroup, was used as a target for PCR amplification. Using RT-PCR, the pair of primers (pal1 and pal2) resulted in amplification of a 660-bp product. RNA samples from African isolates of palyam virus serogroup, propagated in cell cultures, were detected by this RT-PCR-based assay. Amplification product was not detected when the palyam RT-PCR-based assay was applied to RNA from, closely related orbiviruses, bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV); total nucleic acid extracts from uninfected Vero cells. The described RT-PCR-based assay provides a rapid, sensitive and specific method for detection and differentiation of palyam serogroup of orbiviruses in cell culture.
 
 
 
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