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Articles by Mohamad Ramlan Bin Mohamed Salleh
Total Records ( 3 ) for Mohamad Ramlan Bin Mohamed Salleh
  Mohamad Ramlan Bin Mohamed Salleh , Yumi Zuhanis Has-Yun Binti Hashim , Rohani Binti Salleh and Haslinda Binti Hasim
  The purpose of this study was to determine the effect of operating parameters such as the yeast concentration, processing temperature and isolation time on the isolation of GSH using Autolysis method. To study the effect of yeast concentration, yeast solution with the concentration of 15, 30, 45 and 60 wt.% was disrupted at their original temperature for 1 h. The result obtained shows that the optimum value of the concentration for the Autolysis was 15 wt.% with the yield of 0.3942 μmol mL-1 GSH. To study the effect of processing temperature, Autolysis process of the 15 wt.% yeast solution was done at a various values of temperature such as 15, 25, 28, 31, 33 and 40°C. The results indicated that, the optimum GSH isolation temperature (suitable processing temperature) is 28°C with the yield of 1.367 μmol mL-1 GSH. Then, to study the effect of isolation time, the Autolysis process of sample with the concentration of 15 wt.% was done at 28°C for 1, 2 and 3 h. The results indicated that the optimum isolation time for the Autolysis process is 1 h.
  Mohamad Ramlan Bin Mohamed Salleh , Abd. Elaziem Farouk , Rohani Binti Salleh and Haslinda Binti Hasim
  The purpose of this study was to determine the effect of operating parameters such as the yeast concentration, processing temperature and isolation time on the isolation of GSH using Homogenizer Method. The disrupted cells solution was centrifuged at 12,000 rpm for 20 min and the GSH content in the supernatant was analyzed by taking the optical density (OD) value of the solution using Spectrophotometer at 412 nm. Yeast solution with the concentration of 3, 6, 9 and 12 wt.% was disrupted at their original temperature for 30 sec to study the effect of yeast concentration. The result obtained shows that the optimum value of yeast concentration is 9 wt.% where 10.98 μmol mL-1 GSH was isolated. To study the effect of processing temperature, isolation process of the 9 wt.% yeast solution was done at various values of temperature such as 19, 22, 25, 28, 30 and 40°C for 30 sec. The results indicated that, the optimum GSH isolation temperature is 22°C with the isolation of 17.93 μmol mL-1 GSH. Then, to study the effect of isolation time, the isolation process of sample with the concentration of 9 wt.% was done at 22°C for 15, 30, 45 and 60 sec. The result indicated that the optimum isolation time for the Ultrasonic process is 30 sec.
  Mohamad Ramlan Bin Mohamed Salleh , Parveen Jamal , Rohani Binti Salleh and Haslinda Binti Hasim
  The purpose of this study was to determine the effect of operating parameters such as the yeast concentration, processing temperature and isolation time on the isolation of GSH using Ultrasonic method. The disrupted cells solution was centrifuged at 12,000 rpm for 20 min and the GSH content in the supernatant was analyzed by taking the OD value of the solution using Spectrophotometer at 412 nm. To study the effect of yeast concentration, yeast solution with the concentration of 3, 6, 9 and 12 wt.% was disrupted at their original temperature for 15 sec. The result shows that the optimum value of yeast concentration is 6 wt.% where 12.93 μmol mL-1 GSH was isolated. To study the effect of processing temperature, Ultrasonic process of the 6 wt.% yeast solution was done at various values of temperature such as 19, 22, 25, 28, 30 and 40°C for 15 sec. The results indicated that, the optimum GSH isolation temperature is 22°C with the isolation of 13.53 μmol mL-1 GSH. Then, to study the effect of isolation time, the Ultrasonic process of sample with the concentration of 6 wt.% was done at 22°C for 15, 30, 45 and 60 sec. The result indicated that the optimum isolation time for the Ultrasonic process is 15 sec.
 
 
 
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