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Articles by Mirzah
Total Records ( 8 ) for Mirzah
  R. F. Ramadhan , Wizna , Yetti Marlida and Mirzah
  Objective: The aim of this study was to find out the best combination of bovine blood and agri-industrial waste which fermented by Bacillus amyloliquefaciens with different fermentation time to increase the quality of blood mixtures such as protein content, fiber content, nitrogen retention, amino acid composition and enzyme activity. Methodology: Bovine blood and agri-industrial waste obtained from a slaughterhouse and traditional markets. The design used in this study was a Completely Randomized Design (CRD) factorial using three factors. Results: The results showed that there was a highly significant interaction (p<0.01) between blood concentrations, types of agricultural waste and fermentation time on crude protein, nitrogen retention and energy metabolism which the best results was mixtures blood (300/250 mL) and coconut pulp with fermentation time 120 h whereas, the crude protein content, nitrogen retention and enzyme activity (protease and mannanase) were 50.70 and 55.60%, protease 37.34 U g–1 and mannanase 0.992 U g–1, respectively. Meanwhile, the mixtures of blood (300/250 mL) and PKC had the best amino acid composition. Conclusion: The study can be concluded that the best results were mixture of blood meal (300 mL) with coconut pulp and fermentation time 120 h.
  Nuraini , Mirzah and Ade Djulardi
  Background: Marigold (Tagetes erecta) Flower Extract (MFE) is a natural source of carotenoid as a feed additive in the poultry diet. Objective: This study was conducted to determine the effect of MFE as feed additive on production performances and egg quality of quail. Materials and Methods: Two hundred and seven weeks old Coturnix coturnix japonica laying quail were used in a Completely Randomized Design (CRD) with four dietary treatments: 0, 5, 10 and 15 ppm MFE in the diets with five replicates each. Variable measured were feed intake, hen-day egg production, egg weight, egg mass production, feed conversion, egg cholesterol, egg fat and yolk color. Results: It was observed that feed intake, egg production, feed conversion, egg cholesterol and yolk color were significantly affected (p<0.05) by increasing MFE content in the diet. Feed intake, hen-day egg production and yolk color achieved the best results when 15 ppm MFE was included in the diet and resulted in the lowest levels of egg cholesterol and feed conversion. Conclusion: This study shows that 15 ppm MFE in the poultry diet improved production performance and increased egg quality (reduced egg cholesterol and increased egg yolk color) of Coturnix coturnix japonica laying quail.
  R.F. Ramadhan , C. Yang , Wizna , Y. Marlida and Mirzah
  Background and Background: Bovine blood is a livestock by-product that can be used as a protein source for livestock, particularly when incorporated in broiler rations. This study was conducted to investigate the effects of amino acids in Bacillus amyloliquefaciens-fermented mixtures of bovine blood and coconut pulp (blood meal) on growth performance, blood cholesterol and erythrocyte content in broilers. Methodology: A total of 100 six day-old CP 707 (Strain Cobb) broilers were divided into 5 experimental groups with 4 replicates of 5 broilers. The experimental groups received rations supplemented with 0, 5, 10, 15 and 20% blood meal that substituted for fish meal or soybean meal. The experimental period was 5 weeks. Treatment effects on broiler performance parameters such as final body weight, final weight gain, average daily gain, feed intake, average abdominal fat and Feed Conversion Ratio (FCR), total serum cholesterol and erythrocyte count, as well as the return on investment were determined. Results: The group fed rations supplemented with 10% blood meal showed final body weight, final weight gain and FCR of 1,172, 1,035 and 1.75 g, respectively, which was significantly higher (p<0.05) than that for the other treatments. Rations with 10% blood meal also had the best return on investment ($0.92) relative to the other groups. Conclusion: A mixture of 10% bovine blood and coconut pulp fermented with Bacillus amyloliquefaciens can be used in broiler feed without adversely affecting broiler performance and can replace 47% of total fishmeal and 53% of soybean meal in broiler rations.
  Yusuf Mahlil , Husmaini , Warnita , Mirzah and Maria Endo Mahata
  Background and Objective: The processing of dragon fruit (Hylocereus polyrhizus) into a food product leaves fruit peel waste, which is a potential feed for laying hens. Dragon fruit peel contains phytochemical compounds such as anthocyanins, beta-carotene and lycopene, which are reported to have antioxidant functions and to lower cholesterol in blood serum. Previous study showed that the problem with using dragon fruit peel as feed for laying hens was due to its high content of crude fiber, which inhibited the amount of its utilization in the poultry diet and lowered its phytochemical absorption in the digestive tract. Physical and chemical methods, such as steaming and soaking in acid solution, could reportedly degrade and lower the crude fiber content in feed containing high levels of crude fiber. The goal of this study was to evaluate the nutrient content of dragon fruit peel, particularly its crude fiber content, after processing with physical and chemical methods, to determine its use in feed for laying hens. Materials and Methods: The dragon fruit peels in this experiment were collected from local restaurants in Indonesia. The experiment consisted of 2 parts. In part one, dragon fruit peels were steamed in boiling water at a temperature of 98°C. It was performed in a completely randomized design, with different steaming times (0, 5, 10, 15 and 20 min) and each treatment was replicated 4 times. Then the dry matter, crude fiber and crude protein were measured. In the second part, the dragon fruit peels were treated by soaking in 7.5% acetic acid (pH 4). The experiment was performed in a completely randomized design with different soaking times (0, 2, 4, 6 and 8 h) and each treatment was replicated 4 times. The dry matter, crude fiber and crude protein were measured. Results: The experiment showed that both the physical and chemical methods highly significantly reduced the crude fiber content of dragon fruit peel (p<0.01) but did not significantly affect the dry matter or protein content of dragon fruit peel (p>0.05). Conclusion: Processing dragon fruit peels by using a physical method (steaming for 20 min) and a chemical method (soaking in acetic acid solution for 4 h) lowered the crude fiber content in dragon fruit peel from 24.01-19.81% and from 24.01-20.39%, respectively, without altering the protein and dry matter content of dragon fruit peel.
  Nuraini , Mirzah and Ade Djulardi
  Background and Objective: Turmeric (Curcuma domestica, Val) extract (TE) is a natural source of yellow-orange pigment and a natural source of antioxidant, antimicrobial, anti-viral, anti-inflammatory and antitumor compounds. The present study was conducted to evaluate the effect of TE as a feed additive on egg production performances and egg quality of laying quail. Materials and Methods: This study used 208 week old Laying quails. The experiment was performed in a completely randomized design (CRD) with five treatments (0, 5, 10, 15 and 20 ppm TE in drinking water) with four replicates each. The basal diet contained 20% crude protein and 2800 kcal kg–1. The parameters measured were feed intake, hen day-egg production, egg weight, egg mass production, feed conversion, egg yolk color and egg cholesterol content. Results: Increasing the TE level significantly increased (p<0.05) feed intake and hen-day egg production, egg mass production and egg yolk color but significantly decreased (p<0.05) feed conversion and egg cholesterol content. Feed intake, hen-day egg production and yolk color were optimal with the inclusion of 20 ppm TE in the drinking water while egg cholesterol content and feed conversion were lowest at this concentration. Conclusion: This study shows that 20 ppm TE increased the egg production performance and egg quality of laying quail without notable negative effects and therefore should be considered.
  Ucop Haroen , Yetti Marlida , Mirzah and Agus Budianyah
  Antibacterial activity limonoid compounds of three different solvent extracts (methanol, ethyl acetate and n-heksane) prepared by soxhlet extractor from orange waste juice were screened against two pathogenic bacteria Escherichia coli and Salmonella enteridis. The highest antibacterial potentiality was exhibited by the limonoid-ethyl acetate followed by the (limonoid-methanol) and (limonoid-n-hexane).The orange waste juice extract can be considered to be as equally potent as the antibiotics. The zone of inhibition, Minimum Inhibitory Concentration (MIC) of the orange waste juice extracts showed that limonoids extracted with ethyl acetate (limonoids-ethyl acetate) resulted in better inhibition of limonoids extracted with (limonoids-methanol) and (limonoids-n-hexane). The concentration 250 ppm of MIC were test showed inhibition of (limonoids-ethyl acetate) higher with a percentage minimum (44.15 for the bacterium Escherichia coli and 14.69 for the salmonella enteridis) than (limonoids-methanol) (10.67 and 3.84) and (limonoids-n-hexane) (35.58 and 14.10). In vitro antibacterial screening of crude (limonoid-ethyl acetate) (limonoid-methanol) and (limonoid-n-hexane) of methanol extract of orange juice waste with concentration 250 ppm were test showed the clear zone in millimeters (11.75 for the bacterium Escherichia coli and 10.25 for the salmonella enteridis) than limonoid-methanol (8.00 and 9.00) and limonoid-n-hexane (8.00 and 9.25). However, when compared to synthetic antibiotics (neomiditril and coleridin), the clear zone of limonoids lower (24%) but still showed his ability as an anti-microbial. (Limonoids-ethyl acetate) dose of 250 ppm showed better activity than doses of (limonoid-methanol) and (limonoid-n-heksana) for the bacterium Escherichia coli to salmonella enteridis bacterial at 11.918 and 9.158. From these results we can conclude (limonoids-ethyl acetate) extract better than (limonoids-methanol) and (limonoids-n-hexane) and can be used as a substitute for anti biotic synthetic antimicrobial. The phytochemical analysis of orange waste juice extracts showed presence of triter penes, alkaloid, flavonoids, steroid, phenolic, saponin.
  Ucop Haroen , Yetti Marlida , Mirzah and Agus Budianyah
  This study was conducted to evaluate the effect of orange (Citrus sinensis) waste juice extracts (OWJE) on performance in broilers chickens. A total of two hundred and forty unsexed broiler chicks (Arbor Acres CP-707) were randomly allocated to six treatments groups given varying concentrations of OWJE in the drinking water for 35 d. 0, 250, 500, 750 and 1000 ppm OWJE: water supplemented with an antibiotic only was used as a control. The growth responses achieved by broilers from all groups complied with standards. However, supplementation with 1000 ppm OWJE in drinking water increased feed intake and body weight gain, thereby increasing the feed conversion ratio of both starter (days 1-21) and finisher (days 22-35) broilers, while 750 ppm OWJE promoted feed intake and weight gain in starters. These results indicate OWJE is a useful additive for promoting broilers chicken growth. Additional research is needed to improve the suitability of OWJE as a feed additive which promotes growth in poultry.
  Mairizal , Yetti Marlida , Mirzah and Fahmida Manin
  Background and Objective: Mannanase is a mannan-degrading enzyme that can be produced by microorganisms such as bacteria. Mannanase is widely used in the animal feed industry with the aim of improving the quality of feed ingredients containing high levels of mannan, such as palm kernel meal. This study aimed to isolate, characterize and produce mannanase from bacteria found in the hindgut of termites. Materials and Methods: Bacteria were isolated by culturing on solid media containing 0.5% locust bean gum for 24 h at 30°C. The mannanolytic index was then measured by the addition of 2% congo red, which was then washed with 1 M NaCl. Mannanase activity was measured by the dinitrosalicylic acid method, while the protein content was measured by the Bradford method. Results: A total of 8 isolates were obtained. The 3 isolates with the highest mannanolytic indices were selected (isolates V9, AZ and AD). Isolate V9 exhibited the highest mannanase activity of 29.50 U mL–1 after 88 h of incubation with a specific activity of 110.32 U mg–1. The activity of the mannanase was optimal at pH 8 and at an incubation temperature of 50°C. Based on 16S-DNA analysis, isolate V9 was identified as Bacillus cereus. Conclusion: Bacillus cereus, which is isolated from the hindgut of termites, has fairly high mannanase activity and has the potential to hydrolyse mannan in feed ingredients such as palm kernel meal.
 
 
 
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